Recent Votes
Recent Locations •
All
Bologna, 1 minute ago
South Korea, 3 minutes ago
Hong Kong, 4 minutes ago
Cambridge, MA, 9 minutes ago
United States, 10 minutes ago
Adelaide, 12 minutes ago
United States, 17 minutes ago
Recent Awards •
All
Popular Question to
a.basitkhan1990
▴
30
Commentator to
Mensur Dlakic
★
25k
Commentator to
Sasha
▴
770
Popular Question to
bioinfo
▴
140
Scholar to
harold.smith.tarheel
★
4.9k
Popular Question to
carlopecoraro2
★
2.4k
Recent Replies
Comment: How to interpret the discrepancy of assignment rate in featurecounts using forwa
by
charles.feigin
•
0
I'm having this exact same problem. PolyA RNA-Seq library, running with --fr in hisat2 gives a high fraction of concordant single-mappers, …
Comment: How to create a mutation frequency comparison plot?
by
saipra003
▴
10
That's awesome thank you. Found out after a bit of researching that this is called a `Cleveland Dot Plot` if anyone else comes across this.
Comment: Cell ranger multi for demultiplexing FB files and GEX files
by
GenoMax
136k
Demultiplexing is typically done using Illumina software? Have you done this already?: https://www.10xgenomics.com/support/software/cell-ra…
Comment: bbmap split paired-end reads back into separated fastq files?
by
GenoMax
136k
If you simply want to merge "technical" sequencing replicates then you can do the following
cat file1_R1.fastq file1_R1.fastq > merged…
Comment: Best method for batch correction of three datasets
by
CTLong
▴
20
Thanks for the very descriptive reply. I will give it a try nonetheless because this is pretty much the only way to account for the biologi…
Comment: Installing and importing viennaRNA to python in windows
by
Yun
•
0
Just delete "subprocess.", use p = Popen('RNAfold.exe', stdin=PIPE, stdout=PIPE) instead.
Comment: How to resolve the error of protein lacking a stop codon when using GenomeThread
by
peanut
•
0
"Okay.Thank you very much."
Comment: Genome coverage of MAGs while submitting to NCBI
by
wn835166087
•
0
Hi, I have a follow up question about this. During my binning process, I obtained the coverage of each contig. Can I take the average cover…
Comment: Software for Spatial Transcriptomic data [NanoString; Visium]?
by
LauferVA
3.8k
Other relevant posts are gathered here. I'll start sorting through these and redact an answer in a day or two if no more specific answer em…
Comment: Downsampling ATAC-seq BAM files
by
Elise
•
0
Single-nucleus as a part of the 10x multiomics protocol, yes.
Comment: Filling gaps in BAM file
by
seidel
11k
> By gaps, I think I mean just sections of the genome where there was not sufficient coverage or data to produce reliable reads for the BAM…
Comment: ANNOVAR Download Failing
by
Ram
41k
Please verify your internet connectivity using, say, `ping www.google.com`.
Answer: Calculate GC content for entire chromosome
by
ATpoint
78k
https://www.biostars.org/p/358684/#358687
Comment: error when installing gistic2 using conda
by
luckyday1661
•
0
Sure, creating a new environment still does not work, guess the compatibility still needs to be solved, which is most likely not possible b…
Comment: Building reference dbSNP file using WGS samples
by
Brian Bushnell
20k
I've never seen that before so I'm not quite sure how to replicate it. At a minimum, could you give the exact command you used to generate…
Traffic: 1300 users visited in the last hour
Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by the
version 2.3.6
version 2.3.6