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Recent Replies
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C: geneCounts from sorted bam all 0? by
kathryn.eckartt • 10
Yuhp, that's correct- the reference doesn't have a gene-id. There isn't a Chromosome heading in t...
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C: geneCounts from sorted bam all 0? by
swbarnes2 ♦ 5.1k
So your reference does not have gene_IDs? The entry under chromosome in your gff should be "Asse...
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A: How to trim primers with cutadapt from .fastq.gz file? by
Asaf ♦ 5.4k
The adapters you define for the second read are 5' adapters so everything before them will be rem...
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C: geneCounts from sorted bam all 0? by
kathryn.eckartt • 10
If I understand what you're asking, it's the gene_id that's common. The gff lists Chromosome...
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C: Introducing Clumpify: Create 30% Smaller, Faster Gzipped Fastq Files. And remov by
genomax ♦ 64k
Based on your command line this seems to be single-end sequencing data? Even if the start is iden...
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C: How to trim primers with cutadapt from .fastq.gz file? by
lieven.sterck ♦ 4.2k
hi https://www.biostars.org/u/53586/ , I changed the category of your post to 'question' in stead...
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C: geneCounts from sorted bam all 0? by
swbarnes2 ♦ 5.1k
The gene id is in common between your gff and your reference?? Not the chromosome name?
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C: What is the right illumina universal adapter sequence for trimming paired-end re by
maria2019 • 10
Thank you for your answer. I have tried both ways and then I see a good fastQC result with either...
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C: geneCounts from sorted bam all 0? by
kathryn.eckartt • 10
Yuhp, they are both of the same organism from the same assembly. Since the organism is a bacteria...
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A: Number of species for positive selection analysis by
pltbiotech_tkarthi • 140
Maximum Likelihood is a best a method for selection and phylogenetic analysis. Increasing number ...
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C: Should I specify exome region to do the QC with qualimap2 for WES analysis? by
maria2019 • 10
I see. I will check it out. Thanks for your response.
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C: Should I specify exome region to do the QC with qualimap2 for WES analysis? by
ATpoint ♦ 14k
The company that produced the capturing beads must provide this information. Either ask the wetla...
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