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Sanger sequencing chromatogram aligned to reference gene

sanger sequencing alignment reference chromatogram written 7 days ago by vujex • 0 • updated 7 days ago by trausch • 1.5k

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2.7k

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Get trace data for all four bases from ab1 file

sanger trace ab1 chromatogram written 3.0 years ago by st.ph.n • 2.5k • updated 3.0 years ago by sacha • 2.0k

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938

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Is it possible to manipulate Chromatogram ?

sanger fastq chromatogram .abi written 3.4 years ago by mhmtgenc85 • 40

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1.5k

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Any suggestion for sanger sequencing result editing

sanger sequencing chromatogram written 3.4 years ago by liaoyunshi • 0 • updated 3.4 years ago by trausch • 1.5k

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2.0k

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Help: Phred quality for ab1 files

biojava sequence abif phred chromatogram written 4.9 years ago by rabidbirdman4 • 0 • updated 3.8 years ago by trausch • 1.5k

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1.1k

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use R to read CQS file (chromatogram format from Beckman Coulter)

R chromatogram written 3.9 years ago by Timothée Flutre • 20 • updated 3.9 years ago by Jean-Karim Heriche ♦ 24k

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3.0k

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What do the fields DATA.9 - DATA.12 represent in an ab1 file ?

sequencing ab1 chromatogram written 6.1 years ago by anuragm • 130 • updated 6.1 years ago by Dan D ♦ 7.1k

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Recent Replies

A: RNA-seq using DESeq2: quality control of replicates using PCA and heatmaps by swbarnes2 ♦ 9.4k
With only three replicates, you can't omit that sample based solely on your visualizations. Now,...
A: Is it possible to run haplotypecaller in gnu parallel ? by Pierre Lindenbaum ♦ 133k
nextflow (not tested) params.ref="/media/gatk/Homo_sapiens_assembly38.fasta" params.bams...
C: Does GSVA normalize sampleset? by Kevin Blighe ♦ 69k
Yes, I believe some methods result in further normalisation / transformation. This information ca...
C: Is it possible to run haplotypecaller in gnu parallel ? by Pierre Lindenbaum ♦ 133k
use a workflow manager.
C: OMA Standalone 2.4.2: cannot write to .oma directory by adrian.altenhoff • 770
Hi ed, thanks for pointing that out. It is unfortunately not the same problem than the one menti...
A: Is it possible to run haplotypecaller in gnu parallel ? by ole.tange • 3.9k
#!/bin/bash do_one() { gatk --java-options "-Xmx40g" HaplotypeCaller -R /media...
A: RNA-seq using DESeq2: quality control of replicates using PCA and heatmaps by seidel ♦ 7.1k
Your heat map coloring is set to highlight differences between the samples, but the actual correl...
C: RNA-seq using DESeq2: quality control of replicates using PCA and heatmaps by rpolicastro ♦ 3.2k
If you complete analysis, how many DEGs show up? Since PC1 is nicely separating the samples by co...
C: Extract the overlapping paired-end reads by Carlo Yague ♦ 5.5k
You can select overlapping mate pairs based on the template length (9th) field of SAM/BAM files. ...
C: Analyze a gene sequence across the 1000 genomes data by GenoMax ♦ 94k
You can't put in gene sequences. You will need to use region/co-ordinate information to pull out ...
C: add sample name to bam header based on bam file name by Pierre Lindenbaum ♦ 133k
http://www.htslib.org/doc/samtools-addreplacerg.html > -R STRING Allows you to specify the re...
C: add sample name to bam header based on bam file name by Ana • 180
I do not want to change the ID, its the same. How can I adjust it?

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