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Recent Replies
-
C: RNA-Seq, HTSeq-Counts, counts are ~1M less compared to the reference research pa by
GenoMax ♦ 94k
Most NGS aligners are non-deterministic i.e. you may not get exactly identical alignments if you ...
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C: Why did I get very poor scRNAseq files through STARSolo, Galaxy. by
GenoMax ♦ 94k
That may have been one of the problems. Are you simply following the tutorial i.e. not using your...
-
C: trimming of fasta file by
Pierre Lindenbaum ♦ 133k
> God kills a kitten every time you use a gui.
-
C: trimming of fasta file by
Pierre Lindenbaum ♦ 133k
> . in this question I want to isolate the sequence from the middle part which overhangs from ...
-
C: Why did I get very poor scRNAseq files through STARSolo, Galaxy. by
Youyy • 0
Thank you. I am not sure how to extract the code from Galaxy. Last time I didn't pair the R1 and ...
-
C: Why did I get very poor scRNAseq files through STARSolo, Galaxy. by
Youyy • 0
I didn't pair my R1 and R2 files, I uploaded them separately, I am not sure if this is a case. I ...
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C: Merging bowtie output by
ATpoint ♦ 44k
Hello, I am not quite sure I understand. So you want to remove reads of mt origin from this paire...
-
C: How to avoid building millions of BWA indices? by
GenoMax ♦ 94k
If the episome references are non-overlapping then I don't think you can `merge` the BAM files si...
-
C: Pairwise2 Alignment Gap Penalties by
trinityduke100 • 0
Yes but is this for substitutions(mismatch) errors? I have a particular sequence that is passed...
-
C: RNA-Seq, HTSeq-Counts, counts are ~1M less compared to the reference research pa by
xytwwwww • 0
Thank you for your reply! Also, ~1M loss in ~20M sounds very minor to me, would you agree on this?
-
C: trimming of fasta file by
harry • 10
In previous questions, I asked about how to cut a sequence and rejoin. means cut in 2 equal parts...
-
C: Merging bowtie output by
Mark • 850
Use the `samtools merge` to merge the bam files. Do not sure `cat` command.
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