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Answer: Is it Acceptable to Have Uniform Quality Scores in a FASTQ File?
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Comment: Marking duplicates using UMIs
Comment: TCGA2STAT Error: Firehose connection
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Comment: Heatmap and rna-seq
by
Istvan Albert
100k
This forum is dedicated to asking their question on bioinformatics. As long as the topic is bioinformatics, all questions are welcome, and …
Comment: Is it Acceptable to Have Uniform Quality Scores in a FASTQ File?
by
Istvan Albert
100k
I interpret this as an admission by SRA that phred scores are practically useless. The needless granularity (fake precision) was obvious f…
Comment: TCGA2STAT Error: Firehose connection
by
Ram
43k
If their github repo is alive, raise an issue there.
Comment: TCGA2STAT Error: Firehose connection
by
Ngrin
• 0
Yeah makes sense. I was following their github. Thanks for pointing this out.
Comment: The Biostar Herald for Tuesday, April 23, 2024
by
Pierre Lindenbaum
161k
bwa 0.7.18 released ! https://github.com/lh3/bwa/releases/tag/v0.7.18
Comment: Downsampling fastq file
by
Ram
43k
I'm moving this to a comment for now.
Answer: Seurat merge and batch correction
by
bk11
★ 2.3k
You can merge your seven Seurat objects and perform integration using **Seurat V5** to remove the batch effect- ```r library(Seurat) libra…
Comment: How do I use the STARSolo aligner with MGI DNBelab C series HT scRNAseq librarie
by
atowns21
• 0
Yeah, I've been digging around the web for a while now. Can't find any information about the barcodes. The total number of combinations is …
Comment: How do I use the STARSolo aligner with MGI DNBelab C series HT scRNAseq librarie
by
GenoMax
141k
Ah I see that in now. I am not sure why MGI has split the whitelist into two sections. You may need to see if you can dig up some info abou…
Comment: How do I use the STARSolo aligner with MGI DNBelab C series HT scRNAseq librarie
by
atowns21
• 0
read 1 is 30bp... which I think you meant to type. If you scroll approximately halfway down the json file I linked to, it has the following…
Comment: TCGA2STAT Error: Firehose connection
by
Ram
43k
The package has been removed from CRAN and BioConductor. Should you even be using it?
Comment: How do I use the STARSolo aligner with MGI DNBelab C series HT scRNAseq librarie
by
GenoMax
141k
The barcodes are in location 1-10 and you have the actual list there. I don't know for certain what is in position 11-20 (perhaps spacer, c…
Answer: Including plasmid in transcriptome assemblies
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GenoMax
141k
> I want to use both STAR and BOWTIE2 for assembly Those programs are aligners not assemblers. But since you are working with bacteria yo…
Comment: How to slice a gvcf file with tabix?
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Sd
• 0
I already have gvcfs called by chromosome and there are 24 gvcfs per sample which I merged them into a single gvcf per sample. Now I want …
Comment: Find subcluster under a cluster, find differential genes in one cluster between
by
bk11
★ 2.3k
First, could you please explain any reason for not using Seurat V5 approach for your data integration mentioned in [here][1]? > Now moving…
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