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Online Live TScourse: Introduction to Python for Biology (Transmitting Science) July 13th-17th, 2020
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Advanced Sequencing Technologies & Bioinformatics Analysis Course at CSHL: November 3 - 15, 2020
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Research Bazaar 2018
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Recent Votes
- C: Duplicate reads versus unmapped reads
- C: Duplicate reads versus unmapped reads
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Recent Replies
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C: Duplicate reads versus unmapped reads by
pkallurkar • 0
You just made my day. Thanks a ton.
-
C: Duplicate reads versus unmapped reads by
GenoMax ♦ 95k
If you have ~22 M mapped reads in that worst sample then all data should be OK. That is good news...
-
C: Get list of gene signatures published by
igor ♦ 12k
It's unfortunate that the main output of the paper is not more clearly described. Curiously, not...
-
C: Duplicate reads versus unmapped reads by
pkallurkar • 0
The genome is the mouse genome (mm10). The sample with ~70% unmapped reads has ~21.5M uniquely al...
-
C: Error in Saving Files by
anasjamshed1994 • 60
It's python 3, not Python 2. I am used to with Jupiter notebook and thonny
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C: Duplicate reads versus unmapped reads by
GenoMax ♦ 95k
Some samples have very high (70% unmapped) reads so not sure how that is going to affect your dow...
-
C: Duplicate reads versus unmapped reads by
pkallurkar • 0
That's a bummer. Thanks for the reply. Is the data still usable though?
-
C: Duplicate reads versus unmapped reads by
GenoMax ♦ 95k
Those reads are not useful. Perhaps they are a side effect of SMRTseq. So your `STAR` result may ...
-
C: STAR-fusion on galaxy by
avelarbio46 • 10
I tried, but there is no tutorial about it, and no information also. I got many errors, including...
-
C: Strategy for sequencing genomes of nascent polyploid species by
samuel.a.odonnell • 70
If you are after long reads, epigenetic modifications and haplotype phasing, i would say ONT woul...
-
A: Augustus Parsing Loci names and gene names from a Gene bank file format by
h.mon ♦ 32k
Did you copy / paste the perl code directly from the pdf? The problem is you have one (two, actua...
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C: Duplicate reads versus unmapped reads by
pkallurkar • 0
I'm still running mapping for rRNA fasta. But I noticed something in one of the unmapped reads th...
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