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Should I Remove All Positions Containing A Gap In A Multiple Alignment Prior To Creating A Phylogenetic Tree?
Answer: Is it Acceptable to Have Uniform Quality Scores in a FASTQ File?
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Comment: TCGA2STAT Error: Firehose connection
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Answer: Should I use unpaired reads from trimmomatic
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Istvan Albert
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If you have lots of data, both paired and unpaired, then the best strategy is probably to treat them separately and merge the counts at the…
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It is very easy to make nonsensical flags (unfortunately a flaw of the SAM spec itself) ... not to mention the awkward constructs: a primar…
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This forum is dedicated to asking their question on bioinformatics. As long as the topic is bioinformatics, all questions are welcome, and …
Comment: Is it Acceptable to Have Uniform Quality Scores in a FASTQ File?
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Istvan Albert
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I interpret this as an admission by SRA that phred scores are practically useless. The needless granularity (fake precision) was obvious f…
Comment: TCGA2STAT Error: Firehose connection
by
Ram
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If their github repo is alive, raise an issue there.
Comment: TCGA2STAT Error: Firehose connection
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Ngrin
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Yeah makes sense. I was following their github. Thanks for pointing this out.
Comment: The Biostar Herald for Tuesday, April 23, 2024
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bwa 0.7.18 released ! https://github.com/lh3/bwa/releases/tag/v0.7.18
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Ram
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I'm moving this to a comment for now.
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You can merge your seven Seurat objects and perform integration using **Seurat V5** to remove the batch effect- ```r library(Seurat) libra…
Comment: How do I use the STARSolo aligner with MGI DNBelab C series HT scRNAseq librarie
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atowns21
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Yeah, I've been digging around the web for a while now. Can't find any information about the barcodes. The total number of combinations is …
Comment: How do I use the STARSolo aligner with MGI DNBelab C series HT scRNAseq librarie
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GenoMax
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Ah I see that in now. I am not sure why MGI has split the whitelist into two sections. You may need to see if you can dig up some info abou…
Comment: How do I use the STARSolo aligner with MGI DNBelab C series HT scRNAseq librarie
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atowns21
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read 1 is 30bp... which I think you meant to type. If you scroll approximately halfway down the json file I linked to, it has the following…
Comment: TCGA2STAT Error: Firehose connection
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Ram
43k
The package has been removed from CRAN and BioConductor. Should you even be using it?
Comment: How do I use the STARSolo aligner with MGI DNBelab C series HT scRNAseq librarie
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GenoMax
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The barcodes are in location 1-10 and you have the actual list there. I don't know for certain what is in position 11-20 (perhaps spacer, c…
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> I want to use both STAR and BOWTIE2 for assembly Those programs are aligners not assemblers. But since you are working with bacteria yo…
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