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103
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Forum: List of cloud genomics companies
cloud-genomics
updated 11 days ago by 22k • written 10.4 years ago by ★ 3.6k
16
votes
10
replies
1.0k
views
6 follow
Forum: F*up Night style events for Bioinformatics ? Comment if you're interested!
mistakes
updated 22 days ago by 5.3k • written 23 days ago by ★ 2.4k
12
votes
14
replies
2.4k
views
8 follow
Forum: Recovering bam files after unknown deletion in the storage
photorec bam sequencing
written 4.9 years ago by ▴ 70
12
votes
7
replies
6.9k
views
Forum: Differences between SMART-seq2, SMART-seq3, and 10x
cell smartseq UMIs single 10x
updated 26 days ago by ★ 5.8k • written 2.6 years ago by ▴ 70
7
votes
7
replies
879
views
Forum: Bioinformatician who was retaliated against after reporting discriminatory harassment - Please help me navigate getting out of the situation I'm in
Career advice
updated 5 days ago by ★ 2.5k • written 5 days ago by • 0
5
votes
2
replies
371
views
Forum: Can questions and answers on biostars be asked only in English? Can't I use Russian for example?
language
updated 26 days ago by 54k • written 26 days ago by • 0
4
votes
2
replies
244
views
Forum: Bioinformatics: Where do I start
Sequencing
updated 4 days ago by 43k • written 4 days ago by ▴ 10
1
vote
2
replies
323
views
Forum: VCF bulk handling
bcftools SnpEff WGS VEP VCF
3 days ago by Javier • 0
1
vote
1
reply
207
views
Forum: Can I use this community for software development?
software-development
updated 26 days ago by 43k • written 26 days ago by • 0
9 results • Page 1 of 1
Recent Votes
Answer: Mapping cDNA sequence of a single gene to a genome not hosted at UCSC server (wi
Answer: Mapping cDNA sequence of a single gene to a genome not hosted at UCSC server (wi
Answer: MEGA Analysis Issue on High-Performance Computer
Comment: Installing/switching between versions of R/Rstudio/Bioconductor on personal mach
Answer: Ubuntu storage space is decreasing automatically
Comment: How can I retrieve wheat lysine non-acetylated sequences from the UniProt databa
Answer: How can I retrieve wheat lysine non-acetylated sequences from the UniProt databa
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Recent Replies
Comment: Mapping cDNA sequence of a single gene to a genome not hosted at UCSC server (wi by rahu • 0
Thank you for bringing it to my attention! I wasn't aware of GMAP. It seems to be similar to Blat with some proposed advantages, looks very…
Comment: MEGA Analysis Issue on High-Performance Computer by 5heikki 11k
16GB of RAM is nothing. My phone has that. My server has 1TB of RAM, and even that isn't always enough
Comment: MEGA Analysis Issue on High-Performance Computer by GenoMax 141k
I don't think MEGA supports GPU's: https://www.megasoftware.net/web_help_11/Part_I_Getting_Started/Installing_MEGA/System_Requirements.htm
Comment: Installing/switching between versions of R/Rstudio/Bioconductor on personal mach by ATpoint 81k
You can, if you use RStudio via Docker, for example `bioconductor/bioconductor_docker`. I currently manage many different projects with thi…
Comment: bam merging for archaic samples by Matteo Ungaro ▴ 100
@Pierre Lindenbaum I see. So, I can actually manipulate just one single RG tag at the time with `samtools`? Which flag should I use, or sho…
Comment: bam merging for archaic samples by Pierre Lindenbaum 161k
RG tag "L9302" is missing the the BAM header. You should have something like this in the BAM header ``` @RG ID:L9302 SM:L9302 ``` may be…
Answer: Filtering sam or bam file with maximum matching region by Pierre Lindenbaum 161k
> . Is there any way to find through sam or bam file that which chromosomal position are maximum reads aligning to? samtools depth i…
Comment: Calculate Radius of Gyration [python 3] by ndm1096 • 0
Hello Franco. I am very much new with bioinformatics but I have been tasked with studying the radius of gyration of a timeseries of a prote…
Comment: Installing/switching between versions of R/Rstudio/Bioconductor on personal mach by BioinfGuru ★ 1.7k
That makes a lot more sense. Thank you.
Answer: Removing Illumina microarray probes with no expression HumanHT-12v4 by Gordon Smyth ★ 7.0k
Type ``` help("read.idat") ``` and read the example at the bottom of the page, which shows you how to set the `other$Detection` component o…
Comment: Free/open source 23andme-like analysis by swbarnes2 14k
Accurately assessing a few QC metrics is trivially easy. Assessing the significance of variants in a clinically responsible way is really…
Comment: Cellranger count error: Input FASTQ file ended prematurely by swbarnes2 14k
If _1 and _2 really are the same library, you should rename the fastqs, or make symlinks, such that they have the same sample name, but dif…
Comment: Cellranger count error: Input FASTQ file ended prematurely by ATpoint 81k
Just delete the index read files. They just take up space and will never be used.
Comment: Adding CB tag to bam file by swbarnes2 14k
Yes. Why wouldn't you use, say, AddOrReplaceReadsGroups?
Comment: Ciriquant not configuring hisat2 indexed files by Atul K. • 0
Thank you. Decreased the threads to 2 (changed the threads: 2 in the config file; but it was somehow using 4). It did run for sometime but …
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