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460 results • Page 1 of 10
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3
votes
3
replies
181
views
Forum: Ideal PC configurations and operating system for bioinformatics laboratory
PC
updated 1 day ago by 141k • written 1 day ago by • 0
0
votes
0
replies
154
views
Forum: Improvement on automatic annotation of single-cell RNA-seq data from the literature using LLM
annotation scRNA llm
1 day ago by yxwucq • 0
4
votes
2
replies
274
views
Forum: Bioinformatics: Where do I start
Sequencing
updated 8 days ago by 43k • written 8 days ago by ▴ 10
7
votes
7
replies
1.1k
views
Forum: Bioinformatician who was retaliated against after reporting discriminatory harassment - Please help me navigate getting out of the situation I'm in
Career advice
updated 9 days ago by ★ 2.5k • written 10 days ago by • 0
1
vote
2
replies
332
views
Forum: VCF bulk handling
bcftools SnpEff WGS VEP VCF
7 days ago by Javier • 0
16
votes
10
replies
1.0k
views
6 follow
Forum: F*up Night style events for Bioinformatics ? Comment if you're interested!
mistakes
updated 26 days ago by 5.3k • written 27 days ago by ★ 2.4k
1
vote
1
reply
216
views
Forum: Can I use this community for software development?
software-development
updated 4 weeks ago by 43k • written 4 weeks ago by • 0
5
votes
2
replies
382
views
Forum: Can questions and answers on biostars be asked only in English? Can't I use Russian for example?
language
updated 4 weeks ago by 54k • written 4 weeks ago by • 0
0
votes
0
replies
169
views
Forum: Field of Study in Computational Biology
Genomics Computational-Biology PhD
updated 5 weeks ago by 43k • written 5 weeks ago by • 0
1
vote
3
replies
472
views
Forum: Development plan for a bioinformatics database developer/coder
developer
5 weeks ago by pixie@bioinfo ★ 1.5k
7
votes
8
replies
656
views
Forum: Bioinformatics career transition
career forum
updated 5 weeks ago by ★ 16k • written 5 weeks ago by ▴ 20
4
votes
2
replies
469
views
Forum: Exploring Amino Acid Patterns in Proteins Through N-gram Analysis
protein CAFA5
6 weeks ago by Anatoly A. ▴ 10
6
votes
6
replies
753
views
Forum: You have 3 days of zero obligations and expansive resources. What are you building?
hypothetical thought-experiment
7 weeks ago by jared.andrews07 ★ 16k
0
votes
0
replies
160
views
Forum: What other data types and pipelines you would like to see from the Genomics Data Commons (GDC)
gdc tcga genomics-data-commons
updated 7 weeks ago by 43k • written 7 weeks ago by ★ 1.2k
0
votes
2
replies
364
views
Forum: What's the state of the art in bacterial whole genome alignment?
whole genome alignment mauve
7 weeks ago by Dave Carlson ★ 1.7k
31
votes
12
replies
939
views
7 follow
Forum: Nextflow and self-made pipelines opinion
nextflow pipeline data-analysis
7 weeks ago by karlaarz ▴ 90
5
votes
4
replies
546
views
Forum: Would reaching out to local labs and startups help with my career transition?
career volunteering
updated 8 weeks ago by 141k • written 8 weeks ago by ▴ 10
1
vote
2
replies
360
views
Forum: Exploring Career Reentry: Challenges and opportunities for Women After Maternity and Childcare Breaks
Maternity Career-Gap career-reentry
updated 9 weeks ago by 82k • written 9 weeks ago by ▴ 20
8
votes
7
replies
782
views
6 follow
Forum: Tower Server configuration for NGS data analysis
NGS
updated 9 weeks ago by ▴ 120 • written 12 weeks ago by • 0
1
vote
0
replies
272
views
Forum: list of bioinformatics tools in cancer genomics
mutations CNA cancer genomics LOH
updated 9 weeks ago by 161k • written 9 weeks ago by ★ 1.4k
4
votes
2
replies
368
views
Forum: bulk seq vs. sc-seq
scATAC-seq ATAC-seq scRNAseq RNAseq
updated 10 weeks ago by ★ 5.8k • written 11 weeks ago by ▴ 10
8
votes
6
replies
2.8k
views
Forum: Demonstrating Exponential Growth In Pre-School
education
written 10.2 years ago by 10k
0
votes
0
replies
198
views
Forum: tutorial for carrier screening analyses?
carrier-screening
updated 12 weeks ago by 43k • written 12 weeks ago by ▴ 20
2
votes
3
replies
427
views
Forum: My Project
Project CLI MD-Simulations Molecular-docking
12 weeks ago by Ajay A Shettigar • 0
17
votes
11
replies
976
views
Forum: How to overcome the imposter syndrome in bioinformatics?
advice
3 months ago by sil_bioinfo ▴ 40
10
votes
7
replies
2.2k
views
Forum: How to learn bioinformatics?
learning
updated 3 months ago by ★ 1.3k • written 23 months ago by ▴ 20
26
votes
27
replies
2.0k
views
10 follow
Forum: Determining computer time required
computer hpc time
updated 3 months ago by ★ 5.8k • written 3 months ago by ▴ 40
7
votes
6
replies
727
views
Forum: where to work as a bioinformatician (NGS/IA)
employment omics ia ngs
updated 3 months ago by 43k • written 3 months ago by ▴ 20
21
votes
12
replies
3.5k
views
Forum: How to Use Biostars Part-3: Formatting Text and Using GitHub Gists
meta how-to documentation
3 months ago by Ram 43k
2
votes
1
reply
410
views
Forum: Cataloguing available spatial transcriptomics tools, then linking them together into a workflow?
cytassist transcriptomics nanostring xenium visium
3 months ago by LauferVA 4.2k
49
votes
20
replies
2.0k
views
10 follow
Forum: First experiences matter
first-time tolerance
updated 3 months ago by ★ 2.6k • written 3 months ago by ★ 27k
4
votes
3
replies
752
views
Forum: How to get started in bioinformatics
students
updated 3 months ago by 43k • written 3 months ago by ▴ 10
2
votes
2
replies
575
views
Forum: Do batches contain biological variations in omics data (RNAseq or scRNAseq)?
combat single-cell omics batch rnaseq
updated 3 months ago by 43k • written 3 months ago by ▴ 40
0
votes
2
replies
3.2k
views
Forum: I’m considering a career transition from software engineering to bioinformatics. Is it a good fit for me?
career
updated 3 months ago by 141k • written 3 months ago by ▴ 10
5
votes
3
replies
596
views
Forum: Binning the rare biosphere
metagenomics soil assembly techniques binning
updated 4 months ago by ★ 27k • written 4 months ago by ▴ 20
6
votes
2
replies
655
views
Forum: how to start Bioinformatics?
basics
updated 4 months ago by ★ 27k • written 4 months ago by • 0
19
votes
5
replies
1.1k
views
6 follow
Forum: What Are The Most Influential Bioinformatics/Computational Biology Papers Of 2023?
genomics snps survey papers
4 months ago by Raony Guimarães ★ 1.4k
4
votes
6
replies
918
views
6 follow
Forum: Bioinformatics: what do you think of it in the next 20 years?
future
updated 4 months ago by 43k • written 4 months ago by • 0
1
vote
1
reply
486
views
Forum: want to work in open source project/project in bioinformatics
project
updated 4 months ago by 43k • written 5 months ago by • 0
3
votes
5
replies
1.0k
views
Forum: Current Trends in Bioinformatics
structural-bioinformatics
updated 4 months ago by 43k • written 5 months ago by • 0
21
votes
11
replies
2.5k
views
6 follow
Forum: Rust bioinformatics projects ideas
projects scRNA-seq RNA-seq Rust
updated 5 months ago by 22k • written 13 months ago by ▴ 150
176
votes
46
replies
19k
views
26 follow
Forum: Crac: Funny And/Or Weird Names For Bioinformatics Tools
humor
5 months ago by Istvan Albert 100k
3
votes
2
replies
618
views
Forum: RNA-Seq data analysis - question
Python RNA-Seq R
updated 5 months ago by ▴ 10 • written 5 months ago by ▴ 40
0
votes
0
replies
366
views
Forum: How to learn bioinformatics online?
self-education education learning
updated 5 months ago by 43k • written 5 months ago by • 0
6
votes
5
replies
2.3k
views
Forum: Maintaning internal or private installation of Biostar or a similar Q&A site
Biostars
6 months ago by tshtatland ▴ 190
7
votes
9
replies
1.4k
views
6 follow
Forum: Mentor for a beginner bioinformatics student
project mentor
6 months ago by UT_FAN ▴ 40
0
votes
0
replies
443
views
Forum: Steps after doublet detection in single cell data
scRNA doublet
6 months ago by ishwarvh • 0
0
votes
0
replies
350
views
Forum: Automated Checks And QA
retrospective
6 months ago by Sasha ▴ 840
7
votes
13
replies
1.4k
views
Forum: How do you validate and verify your pipeline's software updates?
Validation Verification Quality-Assurance
updated 6 months ago by 43k • written 6 months ago by ▴ 10
21
votes
7
replies
11k
views
Forum: Bioinformatics short paper journal resource [software/webapps/database]
journal software webapp
updated 6 months ago by ▴ 100 • written 8.0 years ago by 11k
460 results • Page 1 of 10
Recent Votes
Comment: clustalo - align milions of short seq, memory issue
Comment: downloading genomes in fasta format from accession ids
Comment: PCA plot (Suggestions Needed)
Answer: PCA plot (Suggestions Needed)
PCA plot (Suggestions Needed)
Comment: To get p-values for the TPM
A: Where To Download Pam50 Gene Set?
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Popular Question to Simone ▴ 10
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Recent Replies
Answer: clustalo - align milions of short seq, memory issue by emmanouil.a ▴ 120
Hi GenoMax and Mensur Dlakic, thank you for your feedback. I'm changing approach in the way to work with a smaller number of seq.
Comment: Annovar using R package by Pierre Lindenbaum 161k
duplicate of your previous question: https://www.biostars.org/p/9592646/ why using R when there are shell-based tools like VEP ? slivar , …
Comment: Trouble with Pilon installation by Lissa Cruz Saavedra • 0
Hi I am trying to install the software but I didn't find the way to clone from github. Could you help me with the pipeline of instruction…
Comment: Adding CB tag to bam file by swbarnes2 14k
I think both these programs are just adding the same tag to every line. I don't think either cares whether it's called RG or CB
Comment: PCA plot (Suggestions Needed) by swbarnes2 14k
Even if this was real data, the tiny numbers for the PC1 and PC2 indicate that the differences between the samples are all absolutely tiny.…
Comment: Creating Synthetic Sequences for a ML Model by biochugs • 0
Thanks for the input. I'll continue to think of more ideas that may be achievable.
Comment: PCA plot (Suggestions Needed) by Jeremy ▴ 890
You could also try kernel PCA, which is a non-linear form of PCA, although this will be more time-consuming.
Comment: Troubleshooting RNA-seq data with DNA contamination by DKA ▴ 40
I have unstranded RNA-seq data. Please share the tool with me, if you do not mind.
Comment: PCA plot (Suggestions Needed) by Mensur Dlakic ★ 27k
Everything that @f1f58aed said, plus that there may be no data manipulation that will give you the clusters you expected. Sometimes there a…
Answer: Creating Synthetic Sequences for a ML Model by Mensur Dlakic ★ 27k
It is a good idea, though I don't see how it would work in practice. I think you will encounter at least one additional problem on top of …
Answer: PCA plot (Suggestions Needed) by Jeremy ▴ 890
Right now, the WT samples are clustering together, but you have one mutant sample off by itself, which could be an outlier. How did you sc…
Comment: Adding CB tag to bam file by GenoMax 141k
@yokofakun had written this: https://www.biostars.org/p/336965/ https://github.com/ruqianl/appendCB may also be useful.
Comment: Troubleshooting RNA-seq data with DNA contamination by GenoMax 141k
Please post information about your tool as a `tools` category post. Don't wait/ask other users to ask you.
Comment: Adding CB tag to bam file by Maria • 0
Sorry for the delay in my answer, I got sidetracked. I am using ```samtools addreplacerg -r "@RG\tID:$cell" -o $cell.bam -h $LINE``` bu…
Answer: Troubleshooting RNA-seq data with DNA contamination by haibol2017 ▴ 10
We just developed a R package to tackle this problem. Do you have stranded or unstranded RNA-seq data contaminated with gDNA? If you wou…
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