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1,000 results • Page
2 of 20
Sort: Votes
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Views
Votes
Replies
70
votes
19
replies
5.5k
views
13 follow
Forum:
Thrown Into A Bioinformatics Position
computational-analysis
updated 20 months ago by
Ram
43k • written 8.5 years ago by
System
▴ 170
69
votes
39
replies
4.5k
views
14 follow
Forum:
Preparation for Bioinformatics.SE closing
meta
biostars
updated 10 months ago by
Ram
43k • written 6.8 years ago by
John
13k
68
votes
48
replies
8.6k
views
16 follow
Forum:
Stranger Things: unexpected limitations of popular tools
samtools
hisat2
updated 10 months ago by
Ram
43k • written 6.7 years ago by
Istvan Albert
100k
68
votes
22
replies
7.7k
views
16 follow
Forum:
Learning Python: list of resources
python
updated 11 months ago by
Ram
43k • written 7.4 years ago by
dago
★ 2.8k
67
votes
21
replies
6.0k
views
8 follow
Forum:
Meta: Markdown in Biostars (now enabled)
meta
12 months ago by
Ram
43k
67
votes
40
replies
9.6k
views
12 follow
Forum:
I want to re-open the old debate: python or perl ?
perl
python
updated 2.1 years ago by
Ram
43k • written 9.4 years ago by
Gabriel R.
★ 2.9k
66
votes
21
replies
6.7k
views
18 follow
Forum:
Is there anyone who was a pure biologist?
next-gen
updated 12 months ago by
Ram
43k • written 9.9 years ago by
Chong Tang
▴ 100
66
votes
29
replies
16k
views
15 follow
Forum:
Fundamentals Of Designing Biological Databases?
database
updated 13 months ago by
Ram
43k • written 13.4 years ago by
anon1234z
▴ 130
65
votes
57
replies
8.0k
views
12 follow
Forum:
Bioinformatics Mobile App
android
genome
iphone
mutations
updated 2.1 years ago by
Ram
43k • written 9.4 years ago by
aeinsights
▴ 20
65
votes
27
replies
7.3k
views
14 follow
Forum:
What is the reason for most software errors in Bioinformatics according to you?
software-error
blast
sequencing
SNP
R
updated 11 months ago by
Ram
43k • written 8.0 years ago by
harne.priyanka
▴ 110
65
votes
26
replies
19k
views
13 follow
Forum:
Macosx For Bioinformatics?
linux
updated 13 months ago by
Ram
43k • written 10.3 years ago by
Eric Normandeau
11k
64
votes
24
replies
2.9k
views
12 follow
Forum:
How much I must feel useless?
machine-learning
Programming
statistics
updated 10 months ago by
Ram
43k • written 5.5 years ago by
Molecular_genetics
▴ 80
64
votes
8
replies
8.2k
views
7 follow
Forum:
Isaac Asimov Accurately Predicts Woes Of Bioinformatician
bioinformatician
updated 13 months ago by
Ram
43k • written 10.9 years ago by
Obi Griffith
20k
63
votes
9
replies
3.9k
views
Forum:
Should The "Post Closing" Option Exist? Vote Now!
meta
updated 13 months ago by
Ram
43k • written 10.8 years ago by
Istvan Albert
100k
63
votes
11
replies
15k
views
9 follow
Forum:
Web Based Interactive Resources To Learn Programming And/Or Bioinformatics
programming
updated 13 months ago by
Ram
43k • written 10.7 years ago by
Malachi Griffith
19k
63
votes
28
replies
3.9k
views
11 follow
Forum:
Bioinformatics analyses & errors: need for better post-publication tools?
next-gen
RNA-Seq
updated 11 months ago by
Ram
43k • written 7.9 years ago by
ablanchetcohen
★ 1.2k
61
votes
16
replies
7.9k
views
14 follow
Forum:
Rust or C++, what to learn after Go for high-performance bioinformatics tools?
library
updated 14 months ago by
Samuel Lampa
★ 1.3k • written 3.4 years ago by
shenwei356
8.4k
61
votes
70
replies
13k
views
21 follow
Forum:
Biostar 2.0 released
meta
biostars
updated 13 months ago by
Ram
43k • written 10.0 years ago by
Istvan Albert
100k
60
votes
28
replies
8.2k
views
27 follow
Forum:
Jim Robinson of the Integrative Genomics Viewer (IGV) uses this
uses-this
updated 12 months ago by
Ram
43k • written 9.5 years ago by
Istvan Albert
100k
60
votes
14
replies
12k
views
16 follow
Forum:
Interesting bioinformatics blogs (2017 edition)
blogs
updated 13 months ago by
Ram
43k • written 7.0 years ago by
WouterDeCoster
47k
59
votes
25
replies
5.2k
views
8 follow
Forum:
Am I crazy, or are most published RNA-seq studies vastly underpowered?
sample-size
statistical-power
RNA-Seq
updated 9 months ago by
Ram
43k • written 3.6 years ago by
telroyjatter
▴ 220
57
votes
15
replies
6.1k
views
9 follow
Forum:
Good practices while dealing with very large datasets
Big-Data
Programming
updated 10 months ago by
Ram
43k • written 6.9 years ago by
VHahaut
★ 1.2k
57
votes
9
replies
5.6k
views
14 follow
Forum:
Handbooks for Bioinformatics Ninjas
gallery
updated 11 months ago by
Ram
43k • written 7.9 years ago by
Shicheng Guo
★ 9.4k
57
votes
15
replies
4.7k
views
9 follow
Forum:
Entice Biologists To Learn R
r
updated 13 months ago by
Ram
43k • written 12.5 years ago by
Madelaine Gogol
5.3k
57
votes
42
replies
6.5k
views
12 follow
Forum:
Why is academic software hard to install?
software-installation
updated 2.2 years ago by
Ram
43k • written 9.5 years ago by
lh3
33k
57
votes
43
replies
16k
views
8 follow
Forum:
Best RNA-Seq aligner: A comparison of mapping tools
NGS
alignment
genome
RNA-Seq
sequence
updated 12 months ago by
Ram
43k • written 5.2 years ago by
David Langenberger
11k
57
votes
19
replies
28k
views
6 follow
Forum:
Attention: Bowtie2 And Multiple Hits
next-gen
bowtie
mapping
updated 13 months ago by
Ram
43k • written 11.4 years ago by
David Langenberger
11k
56
votes
19
replies
4.0k
views
9 follow
Forum:
How to apply for a job
resume
cover-letter
application
CV
updated 10 months ago by
Ram
43k • written 6.7 years ago by
Emily
23k
55
votes
16
replies
5.9k
views
8 follow
Forum:
Mission Impossible: you have 1 minute to analyze the Ebola Genome
ebola
mission-impossible
updated 18 months ago by
Ram
43k • written 9.4 years ago by
Istvan Albert
100k
54
votes
16
replies
5.6k
views
13 follow
Forum:
The new era of bioinformatics: simple and fast tools, fewer and more informative data
kallisto
computational-cost
updated 14 months ago by
Ram
43k • written 8.9 years ago by
Istvan Albert
100k
54
votes
34
replies
4.8k
views
13 follow
Forum:
Should We Release Database Dumps Of All Questions On Biostar?
meta
biostars
updated 13 months ago by
Ram
43k • written 13.3 years ago by
Istvan Albert
100k
52
votes
18
replies
6.3k
views
14 follow
Forum:
Do You Feel Good Or Bad About Putting Your Photo At Biostar?
meta
biostars
updated 13 months ago by
Ram
43k • written 10.5 years ago by
ugly.betty77
★ 1.1k
52
votes
8
replies
4.7k
views
8 follow
Forum:
What Is The Proper Way To Think About Reinventing The Wheel As A Bioinformatician?
software-development
updated 13 months ago by
Ram
43k • written 11.1 years ago by
KCC
★ 4.1k
51
votes
24
replies
9.1k
views
7 follow
Forum:
Bio-informatics salary distribution versus Java salary distribution in the UK
career
updated 2.3 years ago by
Ram
43k • written 9.6 years ago by
William
★ 5.2k
51
votes
16
replies
5.8k
views
11 follow
Forum:
What Are The Most Influential Bioinformatics/Computational Biology Papers Of 2011?
papers
updated 13 months ago by
Ram
43k • written 12.3 years ago by
Farhat
★ 2.9k
50
votes
4
replies
9.2k
views
Forum:
How to add images to a Biostars post
how-to
13 months ago by
Ram
43k
50
votes
8
replies
9.5k
views
6 follow
Forum:
Encode Commentary From Dan Graur
encode
updated 13 months ago by
Ram
43k • written 11.1 years ago by
Damian Kao
16k
50
votes
19
replies
4.9k
views
7 follow
Forum:
Proposal: Biostar Wants To Run Your Ads. Feedback Requested.
biostars
updated 13 months ago by
Ram
43k • written 11.1 years ago by
Istvan Albert
100k
50
votes
20
replies
5.9k
views
18 follow
Forum:
Explain bioinformatics to a 10 year old
bioinformatics
updated 12 months ago by
Ram
43k • written 8.1 years ago by
novice
★ 1.1k
49
votes
27
replies
7.2k
views
10 follow
Forum:
Possible augmented reality tools for bioinformatics and current problems
visualization
3D
augmented-reality
updated 12 months ago by
Ram
43k • written 8.4 years ago by
Peter Leontev
▴ 90
49
votes
19
replies
7.2k
views
13 follow
Forum:
Why Are There No Proteomics Questions In Biostar?
proteomics
biostars
updated 13 months ago by
Ram
43k • written 13.4 years ago by
Jdnavarro
▴ 410
49
votes
20
replies
1.9k
views
10 follow
Forum:
First experiences matter
first-time
tolerance
updated 11 weeks ago by
Michael Love
★ 2.6k • written 12 weeks ago by
Mensur Dlakic
★ 26k
49
votes
36
replies
8.5k
views
11 follow
Forum:
New features and bug fixes for Biostar to work on during Google Summer of Code
meta
biostars
gsoc
updated 13 months ago by
Ram
43k • written 9.9 years ago by
Paolo
▴ 110
48
votes
38
replies
17k
views
8 follow
Forum:
Eukaryotic Genome Annotation in 2016
software
genome
annotation
updated 11 months ago by
Ram
43k • written 8.0 years ago by
Eric Normandeau
11k
47
votes
11
replies
4.1k
views
Forum:
What Operating System Are You Using For Bioinformatic Work?
linux
windows
updated 13 months ago by
Ram
43k • written 10.3 years ago by
Eric Normandeau
11k
47
votes
24
replies
6.4k
views
15 follow
Forum:
What's fascinating about Bioinformatics for potential students?
career
msc
updated 15 months ago by
Ram
43k • written 6.4 years ago by
Michael
54k
47
votes
14
replies
5.0k
views
Forum:
Was FASTA ever popular?
FASTA
alignment
updated 11 months ago by
Ram
43k • written 7.5 years ago by
John
13k
47
votes
30
replies
4.2k
views
10 follow
Forum:
Bioinformatics software distribution
software
updated 10 months ago by
Ram
43k • written 7.1 years ago by
Petr Ponomarenko
★ 2.7k
47
votes
37
replies
8.6k
views
12 follow
Forum:
The Organisation Of Really Contrived Acronyms (Orca)
acronyms
updated 13 months ago by
Ram
43k • written 10.6 years ago by
Niallhaslam
2.3k
47
votes
19
replies
14k
views
10 follow
Forum:
Is Software Development a key part of Bioinformatics?
Software-Development
updated 19 months ago by
Ram
43k • written 9.2 years ago by
xinuke
▴ 70
1,000 results • Page
2 of 20
Recent Votes
Comment: struggle to get fasta files from ucsc goldenPath
Answer: diffbind : killed dba.count(DBsample, bParallel=FALSE)
Quantile normalizing prior to or after TPM scaling?
Structural variant filtering
Trouble with bedtools getfasta
Gnu Parallel - Parallelize Serial Command Line Programs Without Changing Them
Answer: Hybrid assembly Pacbio- Nanopore
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Recent Replies
Comment: struggle to get fasta files from ucsc goldenPath
by
GenoMax
140k
Can you select the output format as sequence instead of BED?
Comment: struggle to get fasta files from ucsc goldenPath
by
Lila M
★ 1.2k
Could you please elaborate how to retrieve the sequences directly from the table browser? Thanks!
Comment: struggle to get fasta files from ucsc goldenPath
by
GenoMax
140k
If coordinates in your BED file refer to chromosomal locations then you need to use the whole genome file and get those sections by the met…
Comment: How to Use Biostars, Part-I: Questions, Answers, Comments and Replies
by
Istvan Albert
100k
maybe it would work better to ask the OP to post their question as a new one and delete this answer after, if you delete the post they ca…
Comment: Issue with making local BLAST database
by
GenoMax
140k
By default only 20 entries are returned if you don't set the `retmax` parameter. You will not want to do this via R since you will be limit…
Comment: struggle to get fasta files from ucsc goldenPath
by
Lila M
★ 1.2k
So this is my approach: I want to interrogate gene PVT1, I know the coordinates for it is chr8:127794532-128101252 In UCSC table I selected…
Comment: Flow Cytometry Data Analysis by Seurat
by
ATpoint
81k
> I think Seurat is suitable for our purpose (e.g. conducting dimensional reduction of the data such as tSNE), but does it make sense? No…
Comment: struggle to get fasta files from ucsc goldenPath
by
GenoMax
140k
mRNA file is not going to have the same co-ordinates as the main chromosomes. Can you clarify what coordinates are in your BED file?
Comment: Downloading a list of all refseq assembly ids, including supressed assembliey
by
GenoMax
140k
Can you provide examples of suppressed ID's?
Comment: struggle to get fasta files from ucsc goldenPath
by
Lila M
★ 1.2k
It is because I want to pull the mRNA fasta files and not DNA (which I guess is the case if I pull it from the entire genome)
Comment: struggle to get fasta files from ucsc goldenPath
by
GenoMax
140k
If your intervals are for main chromosomes why are you using the mRNA file instead of the entire genome? If you have the gene ID's you coul…
Answer: diffbind : killed dba.count(DBsample, bParallel=FALSE)
by
Pierre Lindenbaum
160k
I downsampled the bams and it works now...
Answer: ONT direct RNA sequencing
by
dthorbur
★ 1.7k
You can check the validity of gzipped files using `gzip -v -t file.gz`. Should look something like this: ``` $ gzip -v -t test_file.…
Comment: Nanopore data filtering using fastp
by
emilydolivo97
• 0
I tested seqtk sample but I generate a wird result. Can I share my code and results with you please ?
Comment: Nextflow ERROR : Timeout waiting for connection from pool
by
GenoMax
140k
As best as I can tell it is a combination of things. Did you try setting `export NXF_ENABLE_VIRTUAL_THREADS="true"` in your shell? You also…
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