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1,000 results • Page
3 of 20
Sort: Votes
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Views
Votes
Replies
47
votes
19
replies
14k
views
10 follow
Forum:
Is Software Development a key part of Bioinformatics?
Software-Development
updated 20 months ago by
Ram
43k • written 9.3 years ago by
xinuke
▴ 70
46
votes
12
replies
36k
views
6 follow
Forum:
BWA vs Bowtie 2 (Poll)
BWA
Poll
Bowtie2
updated 23 months ago by
Ram
43k • written 9.1 years ago by
Irene@Sequencing.com
▴ 270
46
votes
9
replies
8.5k
views
10 follow
Forum:
Bioinformatics For Clinicians - What To Teach Them?
education
updated 13 months ago by
Ram
43k • written 10.3 years ago by
j
▴ 140
45
votes
23
replies
6.3k
views
6 follow
Forum:
biostar.slack.com: Chat for the biostars community -- [ feel free to join ]
chat
slack
updated 14 months ago by
Ram
43k • written 6.1 years ago by
Devon Ryan
104k
45
votes
19
replies
2.8k
views
9 follow
Forum:
Where and how NGS techniques are heading for the next 5 years?
next-gen
NGS
updated 11 months ago by
Ram
43k • written 6.9 years ago by
lakhujanivijay
5.8k
45
votes
35
replies
6.1k
views
12 follow
Forum:
looking for a bioinformatics project to do during military rotation
military
updated 22 months ago by
Ram
43k • written 6.6 years ago by
hasani.iut6
▴ 60
44
votes
11
replies
5.2k
views
7 follow
Forum:
Asking The Developers Before Asking Biostars
meta
updated 13 months ago by
Ram
43k • written 10.6 years ago by
Arnaud Ceol
▴ 860
44
votes
11
replies
2.8k
views
6 follow
Forum:
Why can't we downvote on this forum?
meta
biostars
updated 12 months ago by
Ram
43k • written 7.4 years ago by
devenvyas
▴ 740
43
votes
37
replies
2.5k
views
7 follow
Forum:
Checking the quality and reliability in bioinformatics
reproducibility
updated 11 months ago by
Ram
43k • written 6.5 years ago by
zizigolu
★ 4.3k
43
votes
16
replies
4.6k
views
10 follow
Forum:
Need Advice On How To Become A Senior Bioinformatician
advice
career
updated 13 months ago by
Ram
43k • written 10.9 years ago by
calicruz206
▴ 30
42
votes
17
replies
38k
views
15 follow
Forum:
List Of Genome Sequencing Centers/Institutes
genomics
institutes
updated 14 months ago by
Ram
43k • written 11.2 years ago by
Malachi Griffith
19k
42
votes
20
replies
3.4k
views
11 follow
Forum:
What are the most popular NGS tools?
ensembl
NGS
updated 12 months ago by
Ram
43k • written 8.0 years ago by
Emily
23k
42
votes
21
replies
15k
views
10 follow
Forum:
A Database Of Signatures Of Selection In The 1000 Genomes Dataset
1000genomes
human
selection
updated 13 months ago by
Ram
43k • written 10.1 years ago by
Giovanni M Dall'Olio
28k
42
votes
15
replies
7.7k
views
8 follow
Forum:
Directions In Bioinformatics And Web-Based Data Visualization: Revisiting Javascript?
data-visualization
python
r
updated 14 months ago by
Ram
43k • written 11.4 years ago by
Josh Herr
5.8k
42
votes
8
replies
4.7k
views
13 follow
Forum:
What Are The Most Influential Bioinformatics/Computational Biology Papers Of 2017?
sequencing
survey
SNP
updated 14 months ago by
Ram
43k • written 6.3 years ago by
dago
★ 2.8k
41
votes
27
replies
5.9k
views
8 follow
Forum:
Why bioinformaticians need to know programming languages?
Python
R
Programming
updated 11 months ago by
Ram
43k • written 6.9 years ago by
nilo
▴ 90
40
votes
11
replies
7.0k
views
9 follow
Forum:
"An Explosion Of Bioinformatics Careers" - from Science Careers
career
updated 13 months ago by
Ram
43k • written 9.8 years ago by
Pawel Szczesny
3.2k
40
votes
11
replies
4.8k
views
6 follow
Forum:
what is next going to happen in bioinformatics?
current-research
updated 23 months ago by
Ram
43k • written 9.1 years ago by
Mo
▴ 920
40
votes
24
replies
3.8k
views
13 follow
Forum:
What programming language do you use the most in your work? Which one do you like the best?
Programming
Python
R
Scripting
updated 13 months ago by
Ram
43k • written 3.2 years ago by
biotrekker
▴ 100
40
votes
23
replies
4.1k
views
12 follow
Forum:
Why learn programming in bioinformatics?
Programming
updated 11 months ago by
Ram
43k • written 5.6 years ago by
joselu
▴ 110
39
votes
25
replies
4.4k
views
9 follow
Forum:
Making a mac book Bioinfo ready (El Capitan)
mac
software-installation
updated 12 months ago by
Ram
43k • written 8.1 years ago by
Eric Normandeau
11k
39
votes
21
replies
13k
views
10 follow
Forum:
Orientation of PE reads a review of --fr --ff and --rf meanings
RNA-Seq
updated 10 months ago by
Ram
43k • written 5.5 years ago by
Juke34
8.5k
38
votes
19
replies
10k
views
10 follow
Forum:
Kallisto New RNA-seq quantification method discussion
RNA-Seq
updated 12 months ago by
Ram
43k • written 8.0 years ago by
morovatunc
▴ 550
38
votes
17
replies
3.2k
views
7 follow
Forum:
Why does bioinformatics use a special format like a BAM file instead of a database like SQLite?
sam
bam
samtools
updated 10 months ago by
Ram
43k • written 3.7 years ago by
kojix2
▴ 250
38
votes
18
replies
4.2k
views
11 follow
Forum:
Should We Have A Biostar Conference Or Meeting?
conference
biostars
updated 14 months ago by
Ram
43k • written 10.8 years ago by
Michael
54k
38
votes
31
replies
2.8k
views
10 follow
Forum:
To the Biologists out there: What bioinf tool are you missing?
education
career
updated 12 months ago by
Ram
43k • written 7.7 years ago by
mschmid
▴ 180
37
votes
13
replies
3.5k
views
6 follow
Forum:
Bringing Back Downvote
meta
updated 13 months ago by
Ram
43k • written 10.9 years ago by
lh3
33k
37
votes
30
replies
5.0k
views
7 follow
Forum:
Spam In Rss Feeds
meta
RSS
updated 14 months ago by
Ram
43k • written 11.2 years ago by
Madelaine Gogol
5.3k
37
votes
16
replies
4.0k
views
9 follow
Forum:
Sanger sequencing is no longer the gold standard?
Sanger
NGS
updated 11 months ago by
Ram
43k • written 6.5 years ago by
lamteva.vera
▴ 220
36
votes
39
replies
4.1k
views
11 follow
Forum:
Survey/Vote: If you could double the speed of any three commandline tools, which three would they be?
blast
RNA-Seq
alignment
next-gen-sequencing
updated 11 months ago by
Ram
43k • written 6.4 years ago by
dhbradshaw
▴ 130
36
votes
2
replies
6.1k
views
Forum:
Brian Haas of the Trinity Assembler uses this
uses-this
updated 13 months ago by
Ram
43k • written 9.5 years ago by
Istvan Albert
100k
36
votes
34
replies
2.8k
views
9 follow
Forum:
New to bioinformatics.....
gene
updated 11 months ago by
Ram
43k • written 6.1 years ago by
sms.00196
• 0
36
votes
21
replies
19k
views
9 follow
Forum:
What Is Your Favorite Conserved Protein?
protein
conservation
updated 13 months ago by
Ram
43k • written 10.7 years ago by
Mary
11k
36
votes
18
replies
8.1k
views
Forum:
How Does Genomebrowse Free Genome Browser From Golden Helix Compare?
visualization
genome-browser
updated 3.0 years ago by
natay
20 • written 11.1 years ago by
Victor Lyuboslavsky
▴ 140
36
votes
8
replies
3.6k
views
6 follow
Forum:
Time Management Strategies and Advice for Bioinformaticians
professional-skills
updated 12 months ago by
Ram
43k • written 7.9 years ago by
sviatoslav.kendall
▴ 880
36
votes
7
replies
2.9k
views
7 follow
Forum:
What are benefits of Pursuing Bioinformatics over Software Engineering?
career
updated 21 months ago by
Ram
43k • written 8.2 years ago by
graff1994
▴ 10
35
votes
19
replies
3.7k
views
6 follow
Forum:
For the general public seeking advice from this community
diagnosis
rare-disease
updated 19 months ago by
Ram
43k • written 8.6 years ago by
Mary
11k
35
votes
21
replies
3.1k
views
11 follow
Forum:
Should you restrict use of your software based on your political views?
software
updated 19 months ago by
Ram
43k • written 8.6 years ago by
Jean-Karim Heriche
27k
35
votes
19
replies
4.0k
views
Forum:
Crowd Sourcing The 'Analysis' Of The 'Big Foot' Genome
bigfoot
genome
updated 14 months ago by
Ram
43k • written 11.2 years ago by
Will
4.5k
34
votes
8
replies
2.7k
views
9 follow
Forum:
Bioinformatician, a jack of all trades, but in the eye of the CV-beholder a master of none? How can your CV reflect the diverse skills that you have …
cv
industry
career
updated 10 months ago by
Ram
43k • written 4.5 years ago by
'
▴ 300
34
votes
19
replies
7.9k
views
12 follow
Forum:
Programming Language In Bioinformatics
perl
python
updated 13 months ago by
Ram
43k • written 11.0 years ago by
AndreiR
▴ 260
34
votes
22
replies
2.9k
views
11 follow
Forum:
Have we failed as bioinformatician in this time of COVID-19
Bioinformatician
COVID-19
updated 10 months ago by
Ram
43k • written 3.9 years ago by
heididunst
▴ 10
34
votes
13
replies
6.1k
views
11 follow
Forum:
Best Way To Learn Bioinformatics For System Level Programmers.
NGS
HPC
updated 13 months ago by
Ram
43k • written 10.3 years ago by
amjadcsu
▴ 90
34
votes
11
replies
9.3k
views
13 follow
Forum:
Gallery: Computational tools for DNA methylation
computational-tools
methylation
updated 20 months ago by
Ram
43k • written 8.5 years ago by
Shicheng Guo
★ 9.4k
34
votes
23
replies
5.3k
views
8 follow
Forum:
Unbelievably rapid NGS analysis by Churchill
sequencing
NGS
churchill
2.0 years ago by
Ram
43k
34
votes
12
replies
11k
views
9 follow
Forum:
Is going back to the wet lab worth it?
careerwet-lab
updated 12 months ago by
Ram
43k • written 7.3 years ago by
ropolocan
▴ 810
34
votes
5
replies
2.3k
views
Forum:
what we should reply when we are asked do you have any question?
career
interview
updated 11 months ago by
Ram
43k • written 6.9 years ago by
zizigolu
★ 4.3k
34
votes
12
replies
3.7k
views
11 follow
Forum:
How To Change An Expertise In Bioinformatics
career
updated 14 months ago by
Ram
43k • written 13.0 years ago by
Patrick
▴ 220
33
votes
26
replies
3.6k
views
7 follow
Forum:
Poll: How many people have PyPy?
pypy
python
updated 12 months ago by
Ram
43k • written 8.1 years ago by
John
13k
33
votes
5
replies
16k
views
9 follow
Forum:
How do I explain to my non-bioinformatician labmates how to interpret principal components?
RNA-Seq
communication
analysis
pca
updated 20 months ago by
Ram
43k • written 8.3 years ago by
Kristin Muench
▴ 620
1,000 results • Page
3 of 20
Recent Votes
Gene Set Enrichment Analysis
Gene Set Enrichment Analysis
A: Counting Number Of Bases In A Fastq File
Free/open source 23andme-like analysis
Comment: Free/open source 23andme-like analysis
Answer: Missing protein (VEGF-A) in String db
Answer: Missing protein (VEGF-A) in String db
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54k
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Recent Replies
Comment: Ciriquant not configuring hisat2 indexed files
by
Atul K.
• 0
it worked. Thank you. Now my terminal is crashing, seems 16gb of RAM and 4 cores isn't enough. Can you suggest something?
Comment: Adding CB tag to bam file
by
Maria
• 0
I checked Picard ```AddOrReplaceReadsGroups``` and ```AddOATag```. Is that what you're referring to?
Comment: Free/open source 23andme-like analysis
by
Michael
54k
Noted that 'accurate' wasn't among your criteria ;) Anyway, most variant callers are free open-source software. However, for ancestry ana…
Answer: Mapping cDNA sequence of a single gene to a genome not hosted at UCSC server (wi
by
Pierre Lindenbaum
161k
> server so I cannot use Blat for this purpose. blat is avilable as a standane tool: http://hgdownload.soe.ucsc.edu/admin/exe/linux.x86…
Answer: Mapping cDNA sequence of a single gene to a genome not hosted at UCSC server (wi
by
b.contreras.moreira
▴ 160
My favourite for this task would be [GMAP]( http://research-pub.gene.com/gmap ), which can produce GFF format with option `-f 2`. It is…
Answer: Prediction of Ribosomal RNA Genes Using RNAmmer Software
by
colindaven
6.3k
Another alternative is the truly free implementation barrnap https://github.com/tseemann/barrnap. This is also installable via conda so qui…
Comment: Filtration Of Reads With Length Lower Than 30 From Bam
by
Hagen
▴ 10
thanks! This saved me quite some time, I was about to write code to calculate the aligned length from the CIGAR. But this can be done with …
Comment: Per base sequence content failed miserably
by
Kai Xin
• 0
will look into that. Thank you very much
Comment: Korean human genome reference file
by
SeoGyun
• 0
Thanks..... But I want to do imputation, so I have to make it as a vcf file, but the site only has a fatsa file....
Comment: miRNAs quantification using mirdeep2 tool
by
Ashok
• 0
thank you for your reply https://github.com/rajewsky-lab/mirdeep2/blob/master/TUTORIAL.md i saw this github tutorial for miRNA quantifica…
Comment: Load a full GFF3 into annotation track using arrow (Apollo)
by
cmdcolin
★ 3.8k
Hi there, I let a developer know of this thread and they said they can check it out next week. Feel free to post to the https://github.com/…
Comment: How to get the reference panel for UKBB
by
航太郎
• 0
Thanks for your guide!
Comment: Removing duplicates
by
joe
▴ 510
I'd try those OP @samuel recommends My guess is you're new to ONT, fast5 is the 'raw data' which is current over time...other than generat…
Comment: RNA seq differential expression analysis
by
swbarnes2
14k
No. Find a tutorial, try it, then if you have specific questions, ask of the galaxy help site.
Answer: Adding CB tag to bam file
by
swbarnes2
14k
Shouldn't you use PicardTools for this?
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