1
vote
0
replies
82
views
2
votes
0
replies
188
views
2
votes
0
replies
189
views
2
votes
0
replies
216
views
1
vote
0
replies
165
views
2
votes
0
replies
253
views
3
votes
0
replies
186
views
1
vote
0
replies
199
views
2
votes
1
reply
341
views
2
votes
0
replies
264
views
3
votes
0
replies
305
views
3
votes
1
reply
457
views
3
votes
0
replies
269
views
2
votes
0
replies
255
views
3
votes
0
replies
281
views
2
votes
1
reply
293
views
updated 4 months ago by
Istvan Albert
97k
•
written 4 months ago by
Biostar
1.6k
2
votes
0
replies
234
views
7
votes
1
reply
328
views
3
votes
0
replies
270
views
2
votes
0
replies
309
views
4
votes
1
reply
366
views
2
votes
0
replies
312
views
4
votes
0
replies
308
views
0
votes
0
replies
276
views
3
votes
0
replies
258
views
1
vote
0
replies
272
views
1
vote
0
replies
334
views
1
vote
0
replies
292
views
2
votes
0
replies
295
views
2
votes
0
replies
292
views
1
vote
0
replies
339
views
2
votes
0
replies
317
views
2
votes
0
replies
369
views
3
votes
0
replies
353
views
updated 8 months ago by
Istvan Albert
97k
•
written 8 months ago by
Biostar
1.6k
1
vote
0
replies
388
views
1
vote
0
replies
325
views
1
vote
0
replies
323
views
3
votes
0
replies
334
views
1
vote
0
replies
389
views
1
vote
0
replies
376
views
3
votes
0
replies
395
views
3
votes
0
replies
335
views
2
votes
0
replies
370
views
5
votes
0
replies
379
views
1
vote
0
replies
327
views
2
votes
2
replies
472
views
updated 10 months ago by
Istvan Albert
97k
•
written 11 months ago by
Biostar
1.6k
1
vote
0
replies
333
views
3
votes
0
replies
356
views
2
votes
0
replies
336
views
3
votes
0
replies
377
views
Recent Votes
Recent Locations •
All
Japan, just now
France/Nantes/Institut du Thorax - INSERM UMR1087, just now
China, 1 minute ago
Dallas, 1 minute ago
France, 2 minutes ago
Russia, 2 minutes ago
South Korea, 2 minutes ago
Recent Awards •
All
Popular Question to
zoukai3412085
•
0
Teacher to
Jorge Amigo
14k
Popular Question to
Amos Bairoch
▴
110
Popular Question to
CMPanda
▴
20
Autobiographer to
jkim
▴
130
Recent Replies
Answer: demultiplexing with guppy_basecaller and guppy_barcoder using --detect_mid_stran
by
Yuri
•
0
Hi @and did you ever figure out why guppy was resulting in 90% of reads in unclassified folder?
I am running into the same issue, and am…
Comment: Retrieve hgnc_symbol from XM_ refseqs using BiomaRt
by
ladina.hoesli
•
0
Thank you very much, this helps a lot!
Answer: Snakemake vs Nextflow Upcoming bioinformatics Project
by
cfos4698
▴
720
There are a lot of votes for Nextflow here, and it's generally pretty popular (and increasing in popularity). As a 'dissenting' voice, I'll…
Answer: Is it possible to use pseudoalignments.bam to run rMATs?
by
dsull
★
4.0k
No, you can't do that with kallisto's bam files. For one, kallisto (by default) doesn't map to introns which is required by rMATs.
You c…
Answer: genome annotation [sstart] [send] - how to get protein sequence from gene
by
shenwei356
8.0k
It means that the gene is on the negative/minus strand. So you need to compute the reverse complementary sequence before translating it to …
Answer: When to merge multiple fastq files into one for RNAseq analysis?
by
GenoMax
127k
As long as the sample name matches across the lanes they are pieces of the same sample.
> I was thinking of combining the two fastq files…
Comment: Kallisto bustools for scRNA-seq
by
dsull
★
4.0k
Yes, just put that fastq file (it'll be the same as any other kallisto bustools run, except you have just one fastq file and you have that …
Comment: Error building snpEff database "Transcript 'hypothetical_protein' already exist
by
Jane
•
0
Hi,
I have the same issue. After running the command, I run the "sh scripts/buildDbNcbi.sh U00096.2" to download again. Then the same issu…
Answer: bash command to process a line
by
GenoMax
127k
With just one line example not sure if this will work
awk -F '[|_]' '{print $2,$4}' your_file
CU459141.1 CAM87240.1
Comment: RNA Sequencing and Vg pan -transcriptome build
by
kcarery
•
0
Thank you! I found this as well and trying to understand it now.
Comment: RNA Sequencing and Vg pan -transcriptome build
by
kcarery
•
0
Thank you so much! This is where I am leading back to as well! I don't have much insights into Hisat2. I am going to give it a go this week…
Comment: How can I easily remove overlapping transcripts, keeping only longest transcript
by
Juke34
7.8k
Interesting, why would you merge genes that are on different strands? They should be different genes. It cannot be done without modifying t…
Comment: featureCounts problem in reading Gff
by
Beatrice
•
0
That's the output, so apparently I have the exon. Anyway, after running grep command the featureCounts worked, even though I didn't change …
Comment: bash command to process a line
by
saadleeshehreen
▴
110
Yes the word start with lcl| and always sperated by _prot_. I am very naive pattern matching and extraction. I was trying to cut the field …
Traffic: 2385 users visited in the last hour
Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by the
version 2.3.6
version 2.3.6