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Comment: Flow Cytometry Data Analysis by Seurat
C: Which human reference genome should I use?
Which human reference genome should I use?
Comment: Centromere and telomere positions for Chm13v2 assembly
C: Last column in UCSC cytoBands.txt files
Answer: F*up Night style events for Bioinformatics ? Comment if you're interested!
F*up Night style events for Bioinformatics ? Comment if you're interested!
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Recent Replies
Comment: Number of CPUs to use by DRAP for transcriptomic assmbly
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GenoMax
140k
> I have just noticed in the documentation that there is no argument to specify the number of CPUs to use for calculations. If the program…
Comment: Low number of both surviving reads after trimming
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GenoMax
140k
Do you have a plot of adapter contamination for this data from FastQC? If you have a lot of adapter dimers, short inserts i.e. bad quality…
Comment: struggle to get fasta files from ucsc goldenPath
by
GenoMax
140k
Looks like those appear to be "polycomb associated non-coding RNA". You probably know what they are. https://www.ncbi.nlm.nih.gov/nuccore/…
Comment: Parsing fasta file by coordinates
by
GenoMax
140k
> sequences that are from the same region from those that aren't. could refer to sequence composition and that is the reason we were sugge…
Comment: Low number of both surviving reads after trimming
by
Ram
43k
Please edit your post and add the code parts again, this time use the `101010` code button and NOT the double quote button. The latter is u…
Answer: F*up Night style events for Bioinformatics ? Comment if you're interested!
by
Pierre Lindenbaum
160k
... reminds me [https://twitter.com/yokofakun/status/1267877851795259397][1] ( my twitter account is now private) ![ops][2] [1]: https…
Comment: Flow Cytometry Data Analysis by Seurat
by
Ram
43k
Why did you delete this post, https://www.biostars.org/u/142036/ ?
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dariober
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Perhaps more concerning is why you have features (genes?) with small pvalue but fold-change very close to zero. It's also unusual that ther…
Comment: Gene reads all zero for each sample
by
DYLAN NICO
• 0
Thank you for the suggestions. Unfortunately I cannot add any details for lack of information. But I will try using a different alignment a…
Comment: struggle to get fasta files from ucsc goldenPath
by
Lila M
★ 1.2k
They are +ve and -ve strands, but identical two by two ... I can filter that out later on. This is probably a very naive question, but what…
Comment: Gene reads all zero for each sample
by
ATpoint
81k
Do I understand correctly that you have one gene you are interested in, but all counts are zero, right? If so, first thing could be to use …
Answer: ONT direct RNA sequencing
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joe
▴ 470
You should use `cat` on .gz files, part of the magic of .gz ...I guess if you use `zcat` they are unzipped and this is why you get the erro…
Comment: Nanopore data filtering using fastp
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emilydolivo97
• 0
thank you !!
Answer: Can I have multiple active GDC download tokens?
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Zhenyu Zhang
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You can only have one active token at a time, but you can copy the same token as many times as you want and use them in different computers…
Comment: Parsing fasta file by coordinates
by
sorrymouse
▴ 120
Yes, thats correct. A lot of the responses are addressing problems other than the one I have - I don't want to cluster the sequences based …
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