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Recent Replies
Comment: Indexing the Reference Genome is not done in STAR
by
n_navy
•
0
I add these parameters;
#SBATCH -N 4
#SBATCH --tasks=1
#SBATCH -c 4
#SBATCH --time=24:00:00
But it keeps giving error
Comment: RNA seq differential expression analysis
by
rrehimi
•
0
Thanks!
Could you please explain the DEseq2 method using Galaxy step by step?
Input files?
Answer: Korean reference file
by
colindaven
6.3k
Is is this one - KOREF ? There might be multiple versions. https://www.ncbi.nlm.nih.gov/bioproject/PRJNA294231/
If it is, then take that…
Comment: How can I retrieve wheat lysine non-acetylated sequences from the UniProt databa
by
me
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750
The question is not quite clear? are you asking for wheat proteins in UniProtKB which contain a lysine amino-acid, where those lysine are *…
Comment: RNA seq differential expression analysis
by
seta
★
1.9k
You should provide row count (without normalization) for doing DEG analysis by relevant tools such as, edgeR or DEseq.
Comment: GSEA analysis in R
by
Sudip
•
0
Thank you.
I have another question. Is there a way to get the gene list under "setSize" from the output that you gave as an example.
Comment: Per base sequence content failed miserably
by
Kai Xin
•
0
Hi, i have added the fastqc plots (untrimmed and trimmed). The sample was from anaerobes enriched from wastewater treatment plant, and this…
Comment: How can I retrieve wheat lysine non-acetylated sequences from the UniProt databa
by
Pierre Lindenbaum
161k
> lysine non-acetylated sequences
what does it look like in uniprot ? give us an example with a uniprot identifier.
Answer: Indexing the Reference Genome is not done in STAR
by
Pierre Lindenbaum
161k
> CPU time limit exceeded
your command is taking too long and can't be completed.
check your cluster documentation to allocate more …
Comment: Removing duplicates
by
quentinperriere
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thank you for responding.
I was looking for suitable variant calling tool. I'm working on fungus and I don't have fast5 files.
Could you re…
Comment: How to reduce the variation of CIBERSORTx results?
by
feather-W
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Hi LChart,
Thanks for your reply. I see S-mode in the CIBERSORTx official website tutorial(https://cibersortx.stanford.edu/), and the imag…
Comment: How to reduce the variation of CIBERSORTx results?
by
LChart
3.9k
First time I've heard of S-mode. I imagine you've seen this benchmark where it doesn't come out too well: https://academic.oup.com/bib/arti…
Comment: ISCN annotation for SV/CN VCF files
by
adedare
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0
https://res.saletool.cn/vcf/18084521725806
Comment: ISCN annotation for SV/CN VCF files
by
adedare
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https://res.saletool.cn/vcf/18084435127462
Comment: How can I solve this error?
by
22211020193
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I want to conduct GWAS meta-analysis using metal software.
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