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90,413 results • Page
1 of 1809
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0
votes
0
replies
7
views
Error in FUN(left, right) : non-numeric argument to binary operator in RStudio
rstudio
46 minutes ago by
Dimaris
• 0
0
votes
1
reply
31
views
Deeptools, ComputeMatrix and plotProfile
plotprofile
ComputeMatrix
deeptools
Average
updated 1 hour ago by
Devon Ryan
98k • written 3 hours ago by
Ar Es
• 0
1
vote
1
reply
14
views
How to merge SRRs from 1 sample
analysis
NGS
updated 1 hour ago by
GenoMax
99k • written 1 hour ago by
mhenricks
• 0
1
vote
2
replies
31
views
Where to install latest Affymetrix Power Tools?
affymetrix
updated 1 hour ago by
GenoMax
99k • written 3 hours ago by
rebeliscu
• 0
2
votes
7
replies
13k
views
6 follow
HISAT error: Encountered internal HISAT2 exception (#1)
RNA-Seq
updated 2 hours ago by
Federico Giorgi
▴ 640 • written 3.8 years ago by
pixie@bioinfo
★ 1.4k
0
votes
0
replies
20
views
STAR - stringtie procedure
rnaseq
AS
stringtie
splicing
STAR
3 hours ago by
Hojn
• 0
1
vote
2
replies
67
views
Sources of gene expression levels in different tissue types
expression
updated 3 hours ago by
GenoMax
99k • written 4 hours ago by
jacobcvt12
• 0
2
votes
2
replies
86
views
Visualize DNA sequence as a dot plot?
plot
visualization
DNA
dot
updated 3 hours ago by
Alex Reynolds
32k • written 6 hours ago by
jaydu
• 0
0
votes
3
replies
1.8k
views
Do I need to download any specific adapters for Illumina small RNA sequencing kit?
Adapter trimming
small RNA
trimmomatic
cutadapt
updated 4 hours ago by
GenoMax
99k • written 4.1 years ago by
bioinforesearchquestions
▴ 280
580
votes
155
replies
64k
views
89 follow
News:
The Biostar Handbook. A bioinformatics e-book for beginners.
handbook
tutorial
training
News
updated 3 days ago by
Biostar
10 • written 4.4 years ago by
Istvan Albert
87k
2
votes
2
replies
60
views
Deseq2 time interaction term
deseq2
rna-seq
DE
R
updated 4 hours ago by
swbarnes2
9.7k • written 4 hours ago by
townsendae
• 0
0
votes
1
reply
68
views
calculate area under curve for profile plot (deepTools)
plotProlfile
deepTools
updated 4 hours ago by
Devon Ryan
98k • written 5 hours ago by
htchd7ji
• 0
0
votes
4
replies
188
views
Prinseq lite data preprocessing
Data
rna-seq
preprocessing
Forum
Prinseq
lite
updated 4 hours ago by
Ram
32k • written 3 months ago by
Adarsh Kuamr
▴ 40
0
votes
1
reply
45
views
big loss after markduplication
MarkDuplicates
Re-sequencing
Picard
updated 5 hours ago by
GenoMax
99k • written 5 hours ago by
reza
▴ 250
0
votes
2
replies
58
views
remove variable sites from vcf that only differ from reference.
vcf
updated 4 hours ago by
Ram
32k • written 5 hours ago by
goatsrunfaster
▴ 30
9
votes
11
replies
255
views
Forum:
Bioinformatics startup market analysis
startup
4 hours ago by
gtechbio
▴ 30
0
votes
2
replies
52
views
Trimmomatic-0.36 not finding adapter directory
Directory
Trimmomatic
ILLUMINACLIP
updated 7 hours ago by
Istvan Albert
87k • written 7 hours ago by
willbrown1999
• 0
318
votes
53
replies
48k
views
44 follow
What Tools/Libraries Do You Use To Visualize Genomic Feature Data?
visualization
genome
updated 7 hours ago by
cicindel
▴ 70 • written 11.1 years ago by
brentp
23k
1
vote
1
reply
63
views
How comes scRNAseq linear batch corrected data looks worst than before correction?
singlecell
batch
rescalebatches
regressbatches
updated 5 hours ago by
jared.andrews07
9.2k • written 7 hours ago by
Nadine
• 0
0
votes
1
reply
63
views
No annotation peaks when running homer
homer
peaks
tss
updated 8 hours ago by
geneticatt
▴ 120 • written 9 hours ago by
Lila M
▴ 910
0
votes
0
replies
27
views
Parallel haplotyping
gVCF
GATK
HaplotypeCaller
merge
parallel
8 hours ago by
Poshi
• 0
0
votes
0
replies
31
views
How to map human variant to correspoding knock-allele in mouse
HMDC
mouse
MGI
functional_assay
knock-in
3 hours ago by
Damianos P. Melidis
▴ 30
0
votes
1
reply
55
views
How to include the metadata when we build the txDb object?
txdb
r
updated 4 hours ago by
Carambakaracho
★ 2.4k • written 8 hours ago by
qwesxadzc9
• 0
1
vote
2
replies
65
views
question when using topgo
terms
go
incomplete
updated 8 hours ago by
Kevin Blighe
72k • written 13 hours ago by
cyli
• 0
0
votes
0
replies
35
views
Convert genotypes format '12' to ACTG format
r
dplyr
9 hours ago by
api
• 0
1
vote
3
replies
120
views
How to get GO terms to specific to perticular biological process
GO
Enrichment
RNA-seq
updated 9 hours ago by
geneticatt
▴ 120 • written 1 day ago by
BISEP
• 0
0
votes
10
replies
169
views
How to perform multiple alignment using MAFFT?
genome
alignment
gene
sequence
2 hours ago by
anikcropscience
▴ 40
1
vote
3
replies
82
views
How to obtain FDR across all regions investigated in DiffBind
DiffBind
updated 9 hours ago by
Rory Stark
★ 1.0k • written 11 hours ago by
g.persic1991
• 0
0
votes
0
replies
23
views
News:
Online Training - Conservation Genomics
ConservationGenomics
SNPs
Genome
Genomics
9 hours ago by
carlopecoraro2
★ 2.0k
0
votes
1
reply
58
views
Convert GFF3 to splice junction BED file, for filtering variants multi-sample (RNA-seq) VCF
rna
splice-junction
vcf
10 hours ago by
William
★ 4.8k
0
votes
1
reply
35
views
How to find single copy family from InParanoid output
InParanoid
single
copy
updated 9 hours ago by
Dunois
▴ 620 • written 11 hours ago by
gengyang12138
▴ 10
0
votes
0
replies
28
views
GATK 4 and Spark multithreading
multithreading
haplotypecaller
sparks
gatk
11 hours ago by
Victoria
• 0
0
votes
0
replies
26
views
No peaks found in Signac's FindMarker
seurat
atac-seq
Signac
atac
singlecell
11 hours ago by
ahmad mousavi
▴ 520
6
votes
4
replies
122
views
Concatenating fasta files based on prefix ?
aligment
RNA
DNA
fasta
updated 7 hours ago by
5heikki
9.7k • written 12 hours ago by
sunnykevin97
▴ 340
15
votes
10
replies
449
views
How to convert Transcripts ID of rhesus monkey to Transcripts ID of homo sapiens
transcript
rna-seq
updated 9 hours ago by
tamerg
▴ 90 • written 3 days ago by
Quanyou
• 0
0
votes
1
reply
59
views
Database/list of disease complications
complications
database
disease
updated 12 hours ago by
GenoMax
99k • written 14 hours ago by
Nur
• 0
0
votes
2
replies
500
views
Is it possible to annotate a file for the human genome using the command line without using an amino acid sequence
Annotation
human
DNA
PROVEAN
9 hours ago by
kamanovae
• 0
1
vote
1
reply
65
views
Forum:
What is your experience with the customer support of Oxford University Press?
oup
updated 10 hours ago by
dariober
12k • written 12 hours ago by
jiri.hon
▴ 20
1
vote
0
replies
39
views
braker2 output: gff file is missing and species folder is empty
prothint
braker2
genome
braker
annotation
updated 11 hours ago by
zx8754
10k • written 13 hours ago by
praasu
▴ 40
0
votes
2
replies
77
views
Does GISAID fasta files contain spike protein or whole genome sequence?
sequence
protein
spike
genome
updated 11 hours ago by
WouterDeCoster
45k • written 13 hours ago by
er.alruba2017
• 0
1
vote
0
replies
45
views
Job:
ELIXIR Training Coordinator (pan-European bioinformatics training)
lifescience
training
bioinformatics
14 hours ago by
Erin_Ensembl
▴ 420
0
votes
1
reply
50
views
Why GC content is important on NIPT results?
NGS
NIPT
Sequencing
updated 14 hours ago by
German.M.Demidov
★ 2.0k • written 15 hours ago by
khanhlpbao
• 0
1
vote
4
replies
144
views
Variant calling from comparison of assembled genomes where there is only one read depth.
variant
updated 2 hours ago by
samuel.a.odonnell
▴ 130 • written 1 day ago by
Hans
▴ 120
1
vote
1
reply
55
views
Finding all instances of a recurring motif in a repeat protein
repeat
markov
alignment
motifs
updated 15 hours ago by
Mensur Dlakic
★ 10k • written 17 hours ago by
NeuralCode
• 0
0
votes
0
replies
31
views
rpy hgu133a.db loading error
hgu133a.db
python
rpy
15 hours ago by
dxodnd
• 0
2
votes
4
replies
354
views
Help with CNV calling using ExomeDepth
exomedepth
R
CNV
gCNV
exome
updated 16 hours ago by
Joakim
▴ 20 • written 4 months ago by
luffy
• 0
2
votes
1
reply
102
views
How to grep scaffold from the vcf file
introns
VCF
scaffolds
updated 16 hours ago by
Pierre Lindenbaum
135k • written 20 hours ago by
Manoj
▴ 100
2
votes
1
reply
4.3k
views
Forum:
Biostar Forum Posting Guidelines
Forum
written 9.0 years ago by
Istvan Albert
87k
0
votes
1
reply
113
views
For loop - mkdir and command
for
loop
mkdir
updated 17 hours ago by
shenwei356
5.8k • written 19 hours ago by
jellila
• 0
0
votes
1
reply
37
views
News:
Noncoding RNA Conference: upcoming abstract deadline
ncRNA
conference
RNA
noncoding
written 1 day ago by
rory.johnson
▴ 20
90,413 results • Page
1 of 1809
Recent Votes
Answer: Deseq2 time interaction term
Answer: How to merge SRRs from 1 sample
A: How To Find Repeat Expansion Using Exome/Genome Sequencing Data?
Answer: Where to install latest Affymetrix Power Tools?
Comment: Can't count features with featurecount
Comment: Sources of gene expression levels in different tissue types
Comment: Variant calling from comparison of assembled genomes where there is only one r
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Answer: Deeptools, ComputeMatrix and plotProfile
by
Devon Ryan
98k
You would need to merge the samples together, since we otherwise always assume that users want to keep samples separate so they can assess …
Answer: How to merge SRRs from 1 sample
by
GenoMax
99k
You may want to keep them separate in initial steps of qc/trimming through alignment since separate files allow you to work on them in para…
Answer: Where to install latest Affymetrix Power Tools?
by
Mensur Dlakic
★ 10k
Did you ever try Googling [**affymetrix power tools download**][1]? The first hit has download links if you scroll down a bit. [1]: …
Answer: Where to install latest Affymetrix Power Tools?
by
GenoMax
99k
APT binaries are available [on this page][1] from Thermo fisher. [1]: https://www.thermofisher.com/us/en/home/life-science/microarray-a…
Comment: What Coverage allele-fraction threshold to use?
by
kristina.mahan
▴ 130
Can you point me to some statistical tests?
Comment: How to perform multiple alignment using MAFFT?
by
anikcropscience
▴ 40
The variable GENE gets assigned before running the R-script like this: GENE <- "gene_name" DENOVOFOLDER <- "ST16CH_Denovo" pad…
Comment: Variant calling from comparison of assembled genomes where there is only one r
by
samuel.a.odonnell
▴ 130
To call variants from whole genome alignment you may want to look at other tools like minimap2-paftools or MUMmer-dnadiff
Answer: HISAT error: Encountered internal HISAT2 exception (#1)
by
Federico Giorgi
▴ 640
In most cases, the index was made improperly, e.g. using hisat2-build with a gzipped input fasta
Comment: Can Meta-GWAS summary statistic results be used for Meta-GWAS (For example, META
by
Sam
★ 3.4k
If you have the Meta-analyzed results, you shouldn't need the original GWAS as its signal should be incorporated within the result. So fo…
Comment: How to perform multiple alignment using MAFFT?
by
Ram
32k
We're back to square one again. How does the value for the variable `GENE` get assigned? In [this comment][1], you show your R code but not…
Comment: Sources of gene expression levels in different tissue types
by
GenoMax
99k
Illumina human bodymap data v.2: https://www.ebi.ac.uk/gxa/experiments/E-MTAB-513/Results Atlas of RNA sequencing profiles of normal human…
Answer: Sources of gene expression levels in different tissue types
by
ATpoint
47k
https://www.gtexportal.org/home/
Comment: Do I need to download any specific adapters for Illumina small RNA sequencing ki
by
GenoMax
99k
Files currently include with `trimmomatic` should cover all necessary Illumina adapters. With small RNA kits there may be a kit specific …
Answer: Visualize DNA sequence as a dot plot?
by
Alex Reynolds
32k
You could use Python `PIL` (Pillow) to make one: #!/usr/bin/env python ''' pearl_plot.py ''' import sys …
Comment: How to perform multiple alignment using MAFFT?
by
anikcropscience
▴ 40
Ok, here is the R-code that runs MAFFT system(paste0("mafft --maxiterate 1000 Alignment_output/",GENE,".fa > ",GENE,".mafft.fa"))
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