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Comment: Sorting a STAR-output bam file with samtools leads to a significant reduction in
split fasta file to train deep learning model
Comment: split fasta file to train deep learning model
Answer: FastQ file not recognized by hisat2 allignment
Sra Format Questions: How To Get A Sam File That Can Be Converted To Bam?
Sra Format Questions: How To Get A Sam File That Can Be Converted To Bam?
Comment: split fasta file to train deep learning model
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Answer: Where do find virulence gene information in a gff/gtf file?
by
oschwengers
▴ 80
You could try Bakta to annotate your bacterial genome. Internally, it uses AMRFinderPlus and VFDB and potential hits are marked in GFF3 col…
Comment: GIAB Truth Set
by
sheryl
▴ 180
Thanks Jeremy
Answer: GIAB Truth Set
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Jeremy Leipzig
21k
The truthset is an amalgam or consensus of at least 3 different sequencing technologies (Complete Genomics, 10X, Illumina). I'm not sure co…
Comment: split fasta file to train deep learning model
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Mensur Dlakic
★ 22k
This isn't what you are asking, but I will give your my opinion just in case. If you have a single genome, doing a random 80:20 split as y…
Comment: split fasta file to train deep learning model
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GenoMax
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`reformat.sh` from BBTools as well. Sampling parameters: reads=-1 Set to a positive number to only process thi…
Comment: Calculate RPKM
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Chris
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I got gene_length and counts matrix with the same number of row with your suggestion. y <-DGEList(counts=your_data_frame,genes=data.f…
Comment: Handling single-sample VCF after haplotypecaller
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Y.H.P
• 0
Does it mean that before going through combining and final genotyping, all procedures regarding handling VCF files (ex: hard-filtering, BQS…
Answer: FastQ file not recognized by hisat2 allignment
by
Mensur Dlakic
★ 22k
> No such variable: fastq1 Error messages don't come any clearer than this. Your script is expecting a variable named fastq1 to be used …
Comment: Bowtie2 parameters for best alignment
by
kalavattam
▴ 90
It's not necessarily a question of "better" or not. When you perform adapter/quality trimming of reads, you're sidestepping a large part of…
Comment: msigdbr returns the same genesets for mouse as in for human,
by
e.r.zakiev
▴ 30
hello Igor, thanks for the great package. How do I query for mouse sets? `msigdbr::msigdbr(species = "Mus musculus", category = "M8")` r…
Comment: FeatureCounts >edgeR > GO
by
Pegasus
▴ 80
Thank you ATpoint, I modified the main post for more clarification; 1. "When using edgeR and Deseq2 to generate a CSV file for differenti…
Comment: just one plot appears in the shiny application not all
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bkleiboeker
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There's nowhere near enough information/code here for us to know why only one of your many plots aren't showing up. Can you provide a repro…
Comment: FeatureCounts >edgeR > GO
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ATpoint
70k
What is the problem, where do you get stuck?
Comment: Human Population Reference Genomes (+Neanderthal?)
by
emm008
• 0
Thank you for the help! I am unfortunately tied to fasta and blast because I am trying to replicate someone else's methods to sus out the m…
Answer: split fasta file to train deep learning model
by
Matthias Zepper
3.3k
[seqkit sample][1] should be what you a looking for. [1]: https://bioinf.shenwei.me/seqkit/usage/#sample
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