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Recent Replies
Answer: Best way to normalize transcript reads data for PCA & correlation and further bi
by
Jeremy
▴
860
Scaling the data will give you Z-scores. This is a standard way of preparing data for PCA. I would also suggest trying a center-log-ratio…
Comment: How to resolve the error of protein lacking a stop codon when using GenomeThread
by
peanut
•
0
Thank you for your answer,but after verifying the protein file, it seems there is no problem. Even if there are missing codons, the program…
Comment: Finding human .vcf files online to download
by
cmdcolin
★
3.4k
i can't really tell you why your code doesn't work if you dont provide it. and, if you describe as much as possible what your goals are, th…
Answer: Help with gatk CreateSequenceDictionary
by
MatthewP
★
1.3k
maybe try?:
-R /variant_calling/Desktop/demo/supporting_files/hg38/hg38.fa -O /variant_calling/Desktop/demo/supporting_files/hg38/hg3…
Comment: Java error while running HiCDC overview code
by
Brian Bushnell
20k
Looks like you have corrupt or improperly-formatted input. It's expecting to parse a floating-point number where the word "score" appears.…
Comment: what should I do if I get a compromised sample of scRNA-seq data?
by
dsull
★
5.1k
The knee plot is just one type of QC. I'd say just proceed with your analysis and see if you observe anything funky (analysis itself is a Q…
Comment: FastQC without yellow box
by
lobelino
•
0
My question is Can I trust the statistics of my data I didn't get any yellow boxes, but I got high media values.
Why I didn't get any yello…
Answer: FastQC without yellow box
by
ATpoint
78k
The box is the quartiles, the red line the median. Please google how to read a box plot. Your data is good in terms of base quality.
Comment: FastQC without yellow box
by
Ram
41k
What's your question?<a href="" title="Text added because biostars parser needs it"></a>
Comment: vcftools
by
Ram
41k
Deprecated tools don't just stop producing output/errors, that's now how deprecation works.
Comment: vcftools
by
Ram
41k
> couldn't get any output
What does that mean? What did you see on the screen when you ran this command? Did the command run successfully …
Comment: ChatGPT optimized for bioinformatics questions
by
Ram
41k
Wait, tinybio seems more self-aware than I thought:
> **You**: Why did you give me results pertaining to sarek when I never mentioned that…
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