updated 15 hours ago by
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written 15 hours ago by
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Recent Replies
Comment: scRNA-seq: Consistent low number of cells and low fraction reads across the samp
by
GenoMax
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> less than 100 for some samples
Hmm. That is surprising but then in light of that fact ~20% recovery of cells does not sound bad.
Comment: scRNA-seq: Consistent low number of cells and low fraction reads across the samp
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newbee
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Thanks for raising these valid points. I do not have the answer right now. Lately, I heard from the PI that they started with a small numbe…
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A list of InterPro domains is available: https://ftp.ebi.ac.uk/pub/databases/interpro/current_release/entry.list
Answer: DIAMOND tutorial
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`DIAMOND` has a wiki page: https://github.com/bbuchfink/diamond/wiki
Links for DIAMOND papers are here: https://github.com/bbuchfink/diamo…
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AK
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Hi [he1k](https://www.biostars.org/u/139817/),
Please use the following script which downloads and parses the keg data on the fly:
[keg_hi…
Comment: Is there a need to batch correct FPKM or TPM values for within sample comparison
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CTLong
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That's what I would assume, because we are not taking into account cross sample differences here. Thanks
Comment: Is there a need to batch correct FPKM or TPM values for within sample comparison
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CTLong
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That's a good point. So its probably safest to not batch correct for within sample comparisons I guess?
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What is the purpose of doing so? I tried to do that by using the bam file generated by CellRanger. One **important detail** if you are work…
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Thank you - appreciate the translation.
Answer: Guidance on Crucial Parameters for Bioinformatic Analysis of Metabolomic Data
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Em geral, uma taxa de descoberta falsa de 5% (p ajustado<0,05 ) deve ser usada para qualquer teste estatístico em que múltiplas hipóteses i…
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See link here from the developer: https://bioinformatics.stackexchange.com/a/21886/16676
Comment: WGCNA tutorial docs needed: Horvath Lab site down
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Katie
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I would also appreciate the PDFs if anyone has them please
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AMARU
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Hey,
did you solve it? I am having a very similar issue.
Thanks
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I'd start by getting tutorials for R libraries like DESeq, EdgeR, Seurat, etc, and learn by doing.
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