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Comment: Free/open source 23andme-like analysis
by
Michael
54k
Noted that 'accurate' wasn't amongst your criteria ;)
Anyway, most variant callers are free open-source software. However, for ancestry a…
Answer: Mapping cDNA sequence of a single gene to a genome not hosted at UCSC server (wi
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Pierre Lindenbaum
161k
> server so I cannot use Blat for this purpose.
blat is avilable as a standane tool: http://hgdownload.soe.ucsc.edu/admin/exe/linux.x86…
Answer: Mapping cDNA sequence of a single gene to a genome not hosted at UCSC server (wi
by
b.contreras.moreira
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150
My favourite for this task would be [GMAP]( http://research-pub.gene.com/gmap ), which can produce GFF format with option `-f 2`.
It is…
Answer: Prediction of Ribosomal RNA Genes Using RNAmmer Software
by
colindaven
6.3k
Another alternative is the truly free implementation barrnap https://github.com/tseemann/barrnap. This is also installable via conda so qui…
Comment: Filtration Of Reads With Length Lower Than 30 From Bam
by
Hagen
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10
thanks! This saved me quite some time, I was about to write code to calculate the aligned length from the CIGAR. But this can be done with
…
Comment: Per base sequence content failed miserably
by
Kai Xin
•
0
will look into that. Thank you very much
Comment: Korean human genome reference file
by
SeoGyun
•
0
Thanks..... But I want to do imputation, so I have to make it as a vcf file, but the site only has a fatsa file....
Comment: miRNAs quantification using mirdeep2 tool
by
Ashok
•
0
thank you for your reply
https://github.com/rajewsky-lab/mirdeep2/blob/master/TUTORIAL.md
i saw this github tutorial for miRNA quantifica…
Comment: Load a full GFF3 into annotation track using arrow (Apollo)
by
cmdcolin
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3.8k
Hi there, I let a developer know of this thread and they said they can check it out next week. Feel free to post to the https://github.com/…
Comment: Removing duplicates
by
joe
▴
500
I'd try those OP @samuel recommends
My guess is you're new to ONT, fast5 is the 'raw data' which is current over time...other than generat…
Comment: RNA seq differential expression analysis
by
swbarnes2
14k
No. Find a tutorial, try it, then if you have specific questions, ask of the galaxy help site.
Comment: Downloaded pdb's on rcsb.org
by
Ram
43k
Sure. Or, one could do:
bash try.bash "Homo sapiens" AHR | xargs -I v_pdb curl -s -o v_pdb.pdb https://files.rcsb.org/view/v_pdb.pdb
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