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895 results • Page 1 of 18
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795
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167
replies
141k
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109 follow
News: The Biostar Handbook. A bioinformatics e-book for beginners.
training handbook
updated 12 weeks ago by 2.6k • written 7.4 years ago by 100k
122
votes
44
replies
11k
views
24 follow
News: Help Make Biostar Better! Add Your Feedback, Opinions And Suggestions.
meta biostars
updated 5 months ago by 43k • written 11.4 years ago by 100k
24
votes
21
replies
7.5k
views
9 follow
News: Misunderstood parameter of NCBI BLAST
blast alignment
updated 10 months ago by 43k • written 5.6 years ago by ★ 3.4k
46
votes
16
replies
5.9k
views
12 follow
News: Google announces DeepVariant
genome deep-learning google
updated 11 months ago by 43k • written 6.4 years ago by ▴ 990
6
votes
13
replies
2.1k
views
News: Ensembl reduced functionality: 10th March-16th April 2020
BLAT VEP Ensembl service
updated 10 months ago by 43k • written 4.2 years ago by ★ 2.4k
11
votes
11
replies
1.8k
views
8 follow
News: Python Data Visualization Course. Last 3 spots left
next-gen
updated 10 months ago by 43k • written 5.4 years ago by ★ 2.5k
14
votes
11
replies
2.9k
views
6 follow
News: Announcing bioSyntax: Syntax highlighting for biological files
computational-biology
updated 11 months ago by 43k • written 6.3 years ago by ▴ 170
7
votes
11
replies
2.0k
views
News: Bringing the power of Big Data to biomedical researchers
NGS biomedical-research
updated 11 months ago by 43k • written 6.7 years ago by ▴ 100
13
votes
9
replies
2.6k
views
7 follow
News: Inviting NEW Biostars moderators to join Biostars slack channel
slack moderator
6 months ago by GenoMax 141k
11
votes
9
replies
7.7k
views
News: NovaSeq quality analysis
sequencing
updated 11 months ago by 43k • written 6.8 years ago by 20k
7
votes
8
replies
1.9k
views
News: A new Stack Exchange site for Research Computing
unix linux
updated 11 months ago by 43k • written 6.4 years ago by ▴ 690
8
votes
8
replies
2.9k
views
News: Coronavirus Genome Analysis: a new volume in the Biostar Handbook
biostar-handbook education
updated 10 months ago by 43k • written 4.1 years ago by 100k
38
votes
8
replies
1.6k
views
6 follow
News: What happens when a bioinformatician has his own genome sequenced for $300?
DNAseq DNA WGS
updated 10 months ago by 35k • written 10 months ago by 11k
2
votes
7
replies
1.8k
views
News: Beyond pest control: What can we learn from parasites?
docking heme parasitism in-silico
updated 10 months ago by 43k • written 5.1 years ago by 54k
6
votes
7
replies
2.2k
views
News: NCBI wants to remove quality scores from SRA
quality-scores trimming Assembly genome SRA
updated 10 months ago by 43k • written 3.8 years ago by ▴ 40
2
votes
7
replies
1.7k
views
News: Bioinformatics workshop or training courses
RNA-Seq next-gen ChIP-Seq
updated 11 months ago by 43k • written 5.7 years ago by ▴ 140
38
votes
7
replies
2.2k
views
News: 2 last seats available - 7th Berlin Summer School in NGS Data Analysis (September 4-8 in Berlin)
Transcriptomics DNA-Seq Variant-Calling RNA-Seq
8 months ago by David Langenberger 11k
8
votes
6
replies
17k
views
News: Bioinformatics PhD Programs in the US
education PhD
updated 11 months ago by 43k • written 6.4 years ago by ▴ 70
2
votes
6
replies
2.9k
views
News: Master in integrated systems biology / Luxembourg
systems-biology Masters-course
updated 11 months ago by 43k • written 6.7 years ago by ▴ 130
8
votes
6
replies
1.5k
views
News: Illumina to Acquire Pacific Biosciences
pacbio illumina
updated 10 months ago by 43k • written 5.5 years ago by 100k
7
votes
5
replies
2.6k
views
News: Physalia-Course: Introduction to Linux and workflows for biologists (Berlin)
next-gen Unix Assembly alignment
updated 11 months ago by 43k • written 7.1 years ago by ★ 2.5k
9
votes
5
replies
3.6k
views
News: SAMTOOLS 1.4 released
samtools
updated 11 months ago by 43k • written 7.1 years ago by 161k
3
votes
5
replies
1.3k
views
News: Ensembl Release 104 and newer GTF files no longer have genes sorted by position
Ensembl GTF
updated 7 months ago by ▴ 120 • written 17 months ago by ▴ 30
3
votes
5
replies
2.3k
views
News: (18 August - "Enzyme Stability Prediction") A free webinar on on-going CAFA5 (Critical Assessment of Functional Annotation of proteins) challenge
gene-ontology CAFA protein
8 months ago by Alexander ▴ 220
0
votes
5
replies
1.6k
views
News: LAST CALL - 'Introduction to NGS Data Analysis' course in Berlin, October 8-10 2018
NGS training RNA-Seq DNA-Seq
updated 11 months ago by 43k • written 5.9 years ago by 11k
6
votes
4
replies
1.3k
views
News: Course online - Comparative Genomics
assembly genome next-gen alignment
updated 10 months ago by 43k • written 4.0 years ago by ★ 2.5k
9
votes
4
replies
1.6k
views
News: Human Cell Atlas Data Coordination Platform Data Release Questionaire
RNA-Seq
updated 11 months ago by 43k • written 6.2 years ago by ★ 1.8k
1
vote
4
replies
2.0k
views
News: webserver of TCGA data www.tcgaportal.org
genome RNA-Seq TCGA gene
updated 11 months ago by 43k • written 5.9 years ago by ▴ 40
4
votes
4
replies
1.8k
views
News: 12th International Conference on Advancements in Bioinformatics and Drug Discovery
genome Computational-Biology
updated 11 months ago by 43k • written 6.0 years ago by ▴ 20
12
votes
4
replies
2.0k
views
News: #NGSchool2018 summer school in bioinformatics
NGSchool Personalised-Medicine Nanopore
updated 11 months ago by 43k • written 5.9 years ago by ▴ 290
9
votes
4
replies
2.5k
views
7 follow
News: fastp 0.22 released, with new FASTQ deduplication feature.
fastp
updated 8 months ago by 43k • written 2.7 years ago by ★ 2.5k
10
votes
4
replies
1.7k
views
News: 3rd Berlin Summer School in NGS Data Analysis (2 seats just became available)
NGS RNA-Seq DNA-Seq
updated 10 months ago by 43k • written 5.3 years ago by 11k
2
votes
4
replies
1.1k
views
News: Course ONLINE - RNAseq data analysis with R and Bioconductor (15-19 June)
R RNA-seq Bioconductor
updated 10 months ago by 43k • written 3.9 years ago by ★ 2.5k
8
votes
4
replies
1.9k
views
News: supporting vg users on biostars
variation-graph genome vg vgteam
updated 10 months ago by 43k • written 4.6 years ago by ★ 2.4k
0
votes
4
replies
1.0k
views
News: Pan-Structural Variation hackathon in the Cloud!
SV sequencing
updated 10 months ago by 43k • written 4.2 years ago by 9.7k
0
votes
3
replies
1.4k
views
News: :: FINAL CALL :: A Practical Introduction to NGS Data Analysis (Nov 29 - Dec 1, 2023 in Munich, Germany) - only 2 seats left
DNA-Seq NGS RNA-Seq Workshop
5 months ago by David Langenberger 11k
2
votes
3
replies
1.7k
views
News: GCCBOSC 2018: A Bioinformatics Community Conference Call for Abstracts
Galaxy-Community-Conference BOSC GCC
updated 11 months ago by 43k • written 6.2 years ago by ★ 2.2k
0
votes
3
replies
1.0k
views
News: EMBO | EMBL Symposium: Seeing is Believing - Imaging the Molecular Processes of Life
image-analysis
updated 10 months ago by 43k • written 5.5 years ago by ▴ 10
3
votes
3
replies
1.2k
views
News: NCBI's IgBLAST version 1.21.0 is now available
IgBLAST BLAST T-Cell NCBI receptors
11 months ago by PeterC_NCBI ▴ 410
2
votes
3
replies
2.1k
views
News: PDX Models: Tumor-stroma interaction
transcriptome pdx R ChIP-Seq
updated 11 months ago by 43k • written 6.7 years ago by ▴ 70
4
votes
3
replies
3.2k
views
News: -max_target_seqs 1 in NCBI standalone BLAST does not do what you think it does
blast
updated 10 months ago by 43k • written 5.5 years ago by 11k
1
vote
3
replies
1.2k
views
News: 4th Berlin Summer School in NGS Data Analysis - October 5-9, 2020
NGS RNA-Seq DNA-Seq
updated 10 months ago by 43k • written 4.2 years ago by 11k
7
votes
3
replies
3.9k
views
News: Visualizing genomic data: techniques and challenges. Our interview with Dr. Malachi Griffith, Dr. Obi Griffith and Mr. Zachary Skidmore (The Griffith…
R RNA-Seq genome NGSdata
updated 11 months ago by 43k • written 6.8 years ago by ★ 2.5k
4
votes
3
replies
1.0k
views
News: You may need to cleanup duplicate NCBI BLAST nt database volumes on your system
databases NCBI BLAST
7 months ago by PeterC_NCBI ▴ 410
5
votes
3
replies
1.4k
views
News: Top GitHub repos for biomedicine
open-source software GitHub
updated 10 months ago by 43k • written 5.3 years ago by ▴ 50
14
votes
3
replies
3.7k
views
News: Updated: Conference/Symposium/Seminar/Workshop alert 2018-2019
seminar symposium conference
updated 11 months ago by 43k • written 5.9 years ago by 5.8k
4
votes
3
replies
3.3k
views
News: CAFE v4.0 available
phylogenetics CAFE
updated 11 months ago by 43k • written 7.1 years ago by ▴ 150
2
votes
3
replies
1.4k
views
News: Bio-On-Magnetic-Beads (BOMB): Open platform for high-throughput nucleic acid extraction and manipulation
rna-extraction dna-extraction rna-seq
updated 11 months ago by 43k • written 5.6 years ago by ▴ 20
9
votes
3
replies
4.8k
views
News: Bioinformatics Contest 2020. Call for problems
contest
updated 10 months ago by 43k • written 4.6 years ago by ▴ 920
0
votes
2
replies
1.9k
views
News: Webinar: Multi-omics: Challenges, opportunities and application to biomarker discovery
biomarker next-gen-sequencing multiomics cancer
updated 11 months ago by 43k • written 6.8 years ago by ▴ 750
895 results • Page 1 of 18
Recent Votes
Answer: Missing protein (VEGF-A) in String db
Answer: Missing protein (VEGF-A) in String db
Answer: Ciriquant not configuring hisat2 indexed files
Answer: Ciriquant not configuring hisat2 indexed files
DNA Methylation: Sequencing Techniques
Comment: How to install Guppy from Nanopore Technologies?
Comment: SLURM submission problem: Indexing the Reference Genome is not done in STAR
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Recent Awards • All
Popular Question to biwdpang • 0
Popular Question to Michael 54k
Scholar to Gordon Smyth ★ 7.0k
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Recent Replies
Comment: Ciriquant not configuring hisat2 indexed files by Atul K. • 0
it worked. Thank you. Now my terminal is crashing, seems 16gb of RAM and 4 cores isn't enough. Can you suggest something?
Comment: Adding CB tag to bam file by Maria • 0
I checked Picard ```AddOrReplaceReadsGroups``` and ```AddOATag```. Is that what you're referring to?
Comment: Free/open source 23andme-like analysis by Michael 54k
Noted that 'accurate' wasn't among your criteria ;) Anyway, most variant callers are free open-source software. However, for ancestry ana…
Answer: Mapping cDNA sequence of a single gene to a genome not hosted at UCSC server (wi by Pierre Lindenbaum 161k
> server so I cannot use Blat for this purpose. blat is avilable as a standane tool: http://hgdownload.soe.ucsc.edu/admin/exe/linux.x86…
Answer: Mapping cDNA sequence of a single gene to a genome not hosted at UCSC server (wi by b.contreras.moreira ▴ 160
My favourite for this task would be [GMAP]( http://research-pub.gene.com/gmap ), which can produce GFF format with option `-f 2`. It is…
Answer: Prediction of Ribosomal RNA Genes Using RNAmmer Software by colindaven 6.3k
Another alternative is the truly free implementation barrnap https://github.com/tseemann/barrnap. This is also installable via conda so qui…
Comment: Filtration Of Reads With Length Lower Than 30 From Bam by Hagen ▴ 10
thanks! This saved me quite some time, I was about to write code to calculate the aligned length from the CIGAR. But this can be done with …
Comment: Per base sequence content failed miserably by Kai Xin • 0
will look into that. Thank you very much
Comment: Korean human genome reference file by SeoGyun • 0
Thanks..... But I want to do imputation, so I have to make it as a vcf file, but the site only has a fatsa file....
Comment: miRNAs quantification using mirdeep2 tool by Ashok • 0
thank you for your reply https://github.com/rajewsky-lab/mirdeep2/blob/master/TUTORIAL.md i saw this github tutorial for miRNA quantifica…
Comment: Load a full GFF3 into annotation track using arrow (Apollo) by cmdcolin ★ 3.8k
Hi there, I let a developer know of this thread and they said they can check it out next week. Feel free to post to the https://github.com/…
Comment: How to get the reference panel for UKBB by 航太郎 • 0
Thanks for your guide!
Comment: Removing duplicates by joe ▴ 500
I'd try those OP @samuel recommends My guess is you're new to ONT, fast5 is the 'raw data' which is current over time...other than generat…
Comment: RNA seq differential expression analysis by swbarnes2 14k
No. Find a tutorial, try it, then if you have specific questions, ask of the galaxy help site.
Answer: Adding CB tag to bam file by swbarnes2 14k
Shouldn't you use PicardTools for this?
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