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19 results • Page
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News:
NCBI BLAST+ 2.2.15 now available for download
NCBI
Taxonomy
BLAST
6 days ago by
PeterC_NCBI
▴ 370
3
votes
0
replies
237
views
News:
gnomAD v4 released
gnomad
26 days ago by
Pierre Lindenbaum
158k
3
votes
2
replies
2.1k
views
News:
New ebook: From cell line to Command line to learn computational biology
unix
snakemake
computational-biology
RNA-seq
7 days ago by
Ming Tommy Tang
★ 3.6k
1
vote
0
replies
173
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News:
course - Advanced Python Programming
Python-Programming
updated 19 days ago by
Ram
41k • written 19 days ago by
carlopecoraro2
★ 2.4k
1
vote
0
replies
199
views
News:
course - Comparative Genomics
Comparative-Genomics
updated 29 days ago by
Ram
41k • written 4 weeks ago by
carlopecoraro2
★ 2.4k
0
votes
0
replies
108
views
News:
Course - Introduction to R Shiny
R
Shiny
DataVisualisation
6 days ago by
carlopecoraro2
★ 2.4k
0
votes
0
replies
147
views
News:
Genemod - Lab Management Software
lab-management-software
research
updated 14 days ago by
Ram
41k • written 15 days ago by
Genemod
• 0
0
votes
0
replies
115
views
News:
Online expert taught RNA-Seq workshop
workshop
7 days ago by
fturner
▴ 10
0
votes
0
replies
149
views
News:
GWAS course
R
GWAS
updated 19 days ago by
Ram
41k • written 20 days ago by
carlopecoraro2
★ 2.4k
0
votes
0
replies
152
views
News:
Course. - Computational Tumor Evolution
TumorEvolution
CancerGenomics
Bioinformatics
11 days ago by
carlopecoraro2
★ 2.4k
0
votes
0
replies
176
views
News:
Course - Introduction to Machine Learning in R
R
Statistical-Learning
Omics
Machine-Learning
updated 26 days ago by
Ram
41k • written 26 days ago by
carlopecoraro2
★ 2.4k
0
votes
0
replies
140
views
News:
Course - Introduction to Bayesian Data Analysis
R
Statistics
RegressionModeling
Bayesian
22 days ago by
carlopecoraro2
★ 2.4k
0
votes
0
replies
165
views
News:
GWAS course
R
Bioinformatics
GWAS
4 days ago by
carlopecoraro2
★ 2.4k
0
votes
0
replies
113
views
News:
Course - RNAseq in non-model organisms
Pacbio
RNAseq
Nanopore
8 days ago by
carlopecoraro2
★ 2.4k
0
votes
0
replies
180
views
News:
Virtual de novo RNA-seq (transcriptomics) Workshop: November 13-16, 2023
workshop
RNA-seq
transcriptomics
updated 28 days ago by
Ram
41k • written 28 days ago by
mia.nahom
▴ 10
0
votes
3
replies
996
views
News:
:: FINAL CALL :: A Practical Introduction to NGS Data Analysis (Nov 29 - Dec 1, 2023 in Munich, Germany) - only 2 seats left
DNA-Seq
NGS
RNA-Seq
Workshop
14 days ago by
David Langenberger
10k
0
votes
0
replies
189
views
News:
Spatial Omics and Multiplexed Image Analysis course
Spatial-Omics
Multiplexed-Image-Analysis
updated 28 days ago by
Ram
41k • written 28 days ago by
carlopecoraro2
★ 2.4k
0
votes
0
replies
178
views
News:
Next-Generation Sequencing Data Analysis: A Practical Introduction (Nov 29 – Dec 1 in Munich, Germany)
RNAseq
DNAseq
NGS
Workshop
11 days ago by
Lars
★ 1.0k
0
votes
0
replies
144
views
News:
Course: Genome Assembly using Oxford Nanopore Sequencing
GenomeAssembly
Nanopore
Bioinformatics
15 days ago by
carlopecoraro2
★ 2.4k
19 results • Page
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Recent Votes
Comment: Construction of single sequence assembly out of contigs
Answer: featureCounts results: low rate of 'Successfully assigned alignments'
The Biostar Herald for Monday, November 27, 2023
Comment: use bioservices.UniProt (python) to map uniprot accession to [multiple] ensembl
Answer: Best practices for unstranded sequences in featureCounts
Answer: DEseq2 input
Comment: Filter out ALT contigs from CRAM
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Hamtaro
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Recent Replies
Comment: Best practices for differential expression analysis with low-yield Nanopore/ONT
by
dthorbur
▴ 830
I don't have a suggestion for low yield DE analyses, but I am curious about the samples. Did you conduct any ribo-depletion steps prior to …
Answer: How can I map coordinates between two strains of yeast?
by
dthorbur
▴ 830
It sounds like you are looking for synteny between the two genomes. I'm assuming they are assembled, but that may not be the case. If so, […
Comment: Identify clusters of populations based on gene expression
by
Nitin Narwade
★ 1.5k
Why don't you just plot it on your UMAP If it is specific to one cluster/sub-population you should get a relatively high expression in that…
Answer: The difference blastn output when using subject and db options
by
GenoMax
136k
Default `task` option is `megablast` for `blastn` which looks for `highly similar sequences` compared to `blastn` which is looking for `som…
Comment: Discrepancy in total number of bases in trimmed read1 and read2 files after BBDu
by
GenoMax
136k
https://www.biostars.org/u/14684/ may comment on this but it may be the order of operations that is causing this. After the `tpe` is applie…
Comment: Construction of single sequence assembly out of contigs
by
colindaven
5.7k
Inspector (only for long reads) and Merqury for short reads are also pretty good methods for doing a QC of your assembly.
Comment: featureCounts results: low rate of 'Successfully assigned alignments'
by
GenoMax
136k
> why I have soo many multimapping reads? That is a characteristic of the libraries. Not much you can do at this point in process. Just ha…
Comment: unicycler error : [Errno 13] Permission denied:
by
barslmn
★ 2.0k
This is not really a bioinformatics question. Either you're not the owner or in the group of the directory or the directory don't have wr…
Comment: Python function to export the annotated records to genbank file using python and
by
barslmn
★ 2.0k
Could you paste the error as printed to your screen?
Comment: Construction of single sequence assembly out of contigs
by
analyst
▴ 10
I have found dedup.sh script of bbmap tool that removes duplicated sequences. Also there is a seqtk tool. Is it safe to remove duplicated s…
Comment: featureCounts results: low rate of 'Successfully assigned alignments'
by
Hamtaro
▴ 10
Thank you for your reply. I how can I identify why I have soo many multimapping reads? The samples comes from blood from patients under d…
Comment: Construction of single sequence assembly out of contigs
by
analyst
▴ 10
Honestly I have not assessed duplication after assembly. However I used trimmomatic for data filtration before assembly. Trimmomatic param…
Answer: featureCounts results: low rate of 'Successfully assigned alignments'
by
kvn95ss
▴ 20
I could see the following clues from - hisat2 - ```23026867 (61.37%) aligned concordantly >1 times``` And from featureCounts - ``` Mu…
Comment: CPTAC data, download, merge
by
Mohita
▴ 50
I am also facing the same problem. I will be grateful if you help me out for this.
Comment: Circular genome browser
by
giammafer
▴ 20
Thank you very much for organising the 'awesome-genome-visualization' collection. I searched in your database before I made this question …
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