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795
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141k
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109 follow
News: The Biostar Handbook. A bioinformatics e-book for beginners.
training handbook
updated 12 weeks ago by 2.6k • written 7.4 years ago by 100k
122
votes
44
replies
11k
views
24 follow
News: Help Make Biostar Better! Add Your Feedback, Opinions And Suggestions.
meta biostars
updated 5 months ago by 43k • written 11.4 years ago by 100k
81
votes
39
replies
7.5k
views
11 follow
News: State Of Biostar - Future Directions (January 2013)
biostars
updated 14 months ago by 43k • written 11.3 years ago by 100k
59
votes
31
replies
4.9k
views
12 follow
News: Example of how bioinformaticians can publish in Scientific Reports (by nature publishing group) using publicly available NGS data
publication NGS
updated 12 months ago by 43k • written 7.3 years ago by 11k
11
votes
25
replies
6.2k
views
15 follow
News: Questions for the NCBI Next Generation Sequencing Online Workshop Can be Posted with the NCBI_NOW Tag
NCBI_NOW
updated 12 months ago by 43k • written 8.6 years ago by ▴ 330
57
votes
24
replies
6.5k
views
14 follow
News: Join us in building a bioinformatics resource: The Biostar Handbook
biostar-handbook
updated 19 months ago by 43k • written 8.6 years ago by 100k
54
votes
24
replies
4.1k
views
12 follow
News: Big news from Microsoft. BASH on windows.
windows
updated 12 months ago by 43k • written 8.1 years ago by ★ 3.2k
24
votes
21
replies
7.5k
views
9 follow
News: Misunderstood parameter of NCBI BLAST
blast alignment
updated 10 months ago by 43k • written 5.6 years ago by ★ 3.4k
45
votes
19
replies
6.5k
views
8 follow
News: Gamestorming For Bioinformatics
gamestorming
updated 14 months ago by 43k • written 11.6 years ago by 28k
15
votes
18
replies
8.1k
views
6 follow
News: New Free Community Tutorial Website For Bioinformatics Learning
ChIP-seq genome snp RNA-seq
updated 13 months ago by 43k • written 10.7 years ago by ▴ 610
10
votes
17
replies
3.4k
views
7 follow
News: Tell us what you think of the ENA browser?
ena
updated 12 months ago by 43k • written 7.4 years ago by ★ 1.8k
51
votes
17
replies
4.0k
views
News: Biostars 3.0 is now under testing. It has groups, charms and other interesting features.
meta biostars
updated 14 months ago by 43k • written 9.1 years ago by 100k
12
votes
17
replies
4.1k
views
6 follow
News: A programming language for living cells [Cello]
dna sequence research language
updated 12 months ago by 43k • written 8.0 years ago by 11k
21
votes
16
replies
6.2k
views
6 follow
News: Pandora's Toolbox - a collection of bioinformatics programs
Assembly alignment RNA-Seq
updated 2.1 years ago by 43k • written 9.3 years ago by ★ 1.1k
46
votes
16
replies
5.9k
views
12 follow
News: Google announces DeepVariant
genome deep-learning google
updated 11 months ago by 43k • written 6.4 years ago by ▴ 990
11
votes
13
replies
3.1k
views
News: Ensembl datacentre shut-down: Friday 26th August - Tuesday 30th August 2016
Maintenance Ensembl
updated 12 months ago by 43k • written 7.6 years ago by ★ 2.4k
6
votes
13
replies
2.1k
views
News: Ensembl reduced functionality: 10th March-16th April 2020
BLAT VEP Ensembl service
updated 10 months ago by 43k • written 4.2 years ago by ★ 2.4k
48
votes
13
replies
26k
views
10 follow
News: The New Grch38 Human Genome Browser Has Arrived!
ucsc assembly GRCh38 hg38
updated 2.2 years ago by 43k • written 10.1 years ago by 28k
14
votes
11
replies
2.9k
views
6 follow
News: Announcing bioSyntax: Syntax highlighting for biological files
computational-biology
updated 11 months ago by 43k • written 6.3 years ago by ▴ 170
11
votes
11
replies
1.8k
views
8 follow
News: Python Data Visualization Course. Last 3 spots left
next-gen
updated 10 months ago by 43k • written 5.4 years ago by ★ 2.5k
19
votes
11
replies
6.9k
views
News: Solid Software Tools: Denovo Assembly/Xsq Tools Pipeline Mirrored At Biostar
SOLID
updated 14 months ago by 43k • written 11.6 years ago by 100k
7
votes
11
replies
2.0k
views
News: Bringing the power of Big Data to biomedical researchers
NGS biomedical-research
updated 11 months ago by 43k • written 6.7 years ago by ▴ 100
21
votes
11
replies
9.4k
views
6 follow
News: NovaSeq from Illumina (updated info)
illumina NovaSeq
updated 12 months ago by 43k • written 7.3 years ago by 141k
18
votes
11
replies
4.0k
views
News: Full Genome Sequencing Service 30x whole human genome sequencing for $3,490 (project cancelled)
genome next-gen-sequencing
updated 2.3 years ago by 43k • written 9.6 years ago by ▴ 100
8
votes
10
replies
4.0k
views
News: New versions of Minia and DSK (2.0.x)
minia dsk assembly gatb
updated 2.0 years ago by 43k • written 9.2 years ago by ★ 1.5k
22
votes
10
replies
5.5k
views
News: samtools/bcftools/htslib 1.0 released
htslib samtools bcftools
updated 2.3 years ago by 43k • written 9.7 years ago by ★ 2.2k
13
votes
9
replies
2.6k
views
7 follow
News: Inviting NEW Biostars moderators to join Biostars slack channel
slack moderator
6 months ago by GenoMax 141k
11
votes
9
replies
7.7k
views
News: NovaSeq quality analysis
sequencing
updated 11 months ago by 43k • written 6.8 years ago by 20k
27
votes
9
replies
4.1k
views
News: OSX 10.11 released 30th Sep will be "rootless"
mac osx
updated 19 months ago by 43k • written 8.6 years ago by 13k
17
votes
9
replies
3.8k
views
News: My Ncbi Curriculum Vitae Web Application: Sciencv
ncbi
updated 13 months ago by 43k • written 10.6 years ago by 161k
22
votes
9
replies
6.7k
views
News: Epigenetics Workshop - DNA Methylation Data Analysis
bisulfite-sequencing workshop epigenetics
updated 15 months ago by 43k • written 8.9 years ago by 11k
0
votes
9
replies
2.3k
views
News: Practical Genomics: From Biology to Biostatistics Workshop (Baltimore, MD)
workshop
updated 13 months ago by 43k • written 9.8 years ago by 10k
6
votes
9
replies
4.2k
views
News: 1000 Genomes : The Initial Phase 3 variant list and phased genotypes.
1000genomes
updated 13 months ago by 43k • written 9.8 years ago by 161k
38
votes
8
replies
1.6k
views
6 follow
News: What happens when a bioinformatician has his own genome sequenced for $300?
DNAseq DNA WGS
updated 10 months ago by 35k • written 10 months ago by 11k
12
votes
8
replies
3.4k
views
News: 'Potential Rna-Dna Binding Site In Alu Repeat', Or 'Why Should I Allow Multiple Mapping Positions When Analyzing Chipseq Data?'
chipseq rna
updated 13 months ago by 43k • written 10.8 years ago by 11k
8
votes
8
replies
2.9k
views
News: Coronavirus Genome Analysis: a new volume in the Biostar Handbook
biostar-handbook education
updated 10 months ago by 43k • written 4.1 years ago by 100k
7
votes
8
replies
1.9k
views
News: A new Stack Exchange site for Research Computing
unix linux
updated 11 months ago by 43k • written 6.4 years ago by ▴ 690
13
votes
8
replies
2.7k
views
News: NCBI is going to eliminate GI identifiers
ncbi GI
updated 12 months ago by 43k • written 8.1 years ago by ★ 2.9k
12
votes
8
replies
7.1k
views
News: rRNA Depletion / Poly-A Selection Responsible for RNA-seq Bias
rRNA-depletion RNA-seq-bias
updated 13 months ago by 43k • written 9.8 years ago by ▴ 650
7
votes
8
replies
4.1k
views
News: Next-Gen Sequencing Tutorials On Cubelp (Cranfield Bioinformatics Electronic Learning Platform)
genome chip-seq snp assembly
updated 18 months ago by 43k • written 10.7 years ago by ▴ 610
27
votes
7
replies
3.6k
views
News: The Biostar Swarm: A Visualization
biostars
updated 14 months ago by 43k • written 11.2 years ago by 100k
23
votes
7
replies
4.5k
views
News: Research accounts on GitHub
github git
updated 13 months ago by 43k • written 9.9 years ago by 161k
17
votes
7
replies
9.0k
views
News: Upcoming NGS Workshop: A Beginner's Guide to NGS Data Analysis
workshop Assembly transcriptomics RNA-Seq
updated 2.1 years ago by 43k • written 9.4 years ago by 11k
21
votes
7
replies
7.7k
views
News: Survey: Rna-Seq Analysis For Differential Gene/Transcript Expression [Updated With Results]
transcript RNA-seq gene
updated 13 months ago by 43k • written 11.4 years ago by ▴ 250
24
votes
7
replies
4.4k
views
News: #Bioinformatics Crowdcoding
crowd-coding
updated 13 months ago by 43k • written 10.8 years ago by 161k
25
votes
7
replies
2.8k
views
6 follow
News: Who qualifies to be a bioinformatician?
resume bioinformatician vc
updated 21 months ago by 43k • written 9.0 years ago by 161k
13
votes
7
replies
5.8k
views
News: Free HiSeq X Ten human genome fastq test data
HiSeq-X-Ten Human-genome Fastq next-gen
updated 2.4 years ago by 43k • written 9.7 years ago by ★ 1.8k
10
votes
7
replies
2.8k
views
News: Linux for Biologists e-book (free to read online)
linux python galaxy conda virtual-machine
2.5 years ago by vimalkvn ▴ 320
25
votes
7
replies
6.3k
views
News: Pubmed Commons : A System That Enables Researchers To Share Their Opinions About Scientific Publications
ncbi pubmed
updated 13 months ago by 43k • written 10.5 years ago by 161k
38
votes
7
replies
2.2k
views
News: 2 last seats available - 7th Berlin Summer School in NGS Data Analysis (September 4-8 in Berlin)
Transcriptomics DNA-Seq Variant-Calling RNA-Seq
8 months ago by David Langenberger 11k
1,000 results • Page 1 of 20
Recent Votes
High fold change values
C: Bcftools Mpileup Removes Marked Duplicates?
Prediction tools summary - zero values
A: Error with Formatdb of legacy blast?
Error with Formatdb of legacy blast?
Answer: How to convert SAM/BAM file to GTF/GFF file?
A: Ribo-seq vs RNA-seq read count
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Popular Question to anasjamshed ▴ 120
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Recent Replies
Comment: ISCN annotation for SV/CN VCF files by adedare • 0
https://res.saletool.cn/vcf/18084435127462
Comment: How can I solve this error? by 22211020193 • 0
I want to conduct GWAS meta-analysis using metal software.
Comment: How to reduce the variation of CIBERSORTx results? by feather-W • 0
Hi LChart, Thanks for your reply. Yes, the identical command lines produce different results in CIBERSORTx S-mode. Although I input the da…
Comment: Removing duplicates by joe ▴ 490
I *could* imagine an ONT scenario where you'd want to remove duplicates (amplicon-seq, etc) but likely for variant calling there is no need…
Comment: Removing duplicates by samuel.a.odonnell ▴ 510
As above, you do not need to worry about duplicates for ONT data Also have you looked at using other tools for variant calling more suited…
Answer: Pediococcus acidilactici sequence analysis by geneontologyhelp ▴ 340
If you're looking for the GO annotations for *Pediococcus acidilactici*, these are available. Using the instructions under [*2. All other…
Answer: What is the normalization status of metabric data? by etiennedanis ▴ 10
I just found an answer to this question here: [https://groups.google.com/g/cbioportal/c/hm_i4jbG1uc/m/h9rNYOvpAAAJ][1] [1]: https://gro…
Comment: Removing duplicates by joe ▴ 490
With ONT you will only have PCR duplicates. 'Read' (you mean optical?) duplicates are an artifact of cluster-based sequencing (ie Illumina)…
Comment: assembly using CCS, CLR, CCS_CLR sequences together? by GenoMax 141k
PacBio has some recommendations on software for genome assemblies on this page (which you may have seen but just in case): https://www.pacb…
Answer: DEG analysis of RNA-seq data across multiple tissues and two conditions by swbarnes2 14k
I would not put totally different tissues in the same DESeq object. I don't think that's going to do good things for normalization or disp…
Comment: Removing duplicates by quentinperriere • 0
pcr duplicates or/and read duplicates should I use this command to remove them ? or we don't talk about duplicates when dealing with ONT ??…
Comment: How to reduce the variation of CIBERSORTx results? by LChart 3.9k
If you didn't change the input signature matrix, the input RNA matrix, or the input parameters - what did you change? Is the statement that…
Answer: Inconsistency in SNP detection pipelines for multi-sample analysis by LChart 3.9k
> To replicate GVCF behavior and avoid issues with 0/0 genotypes appearing as missing, I don't use the -v option I'm not sure what you m…
Comment: Supergnova stuck in tutorial by GenoMax 141k
Your best option may be to email the author (and/or the senior author from the paper). Let them know that the FTP links mentioned on GitHub…
Comment: DEG analysis of RNA-seq data across multiple tissues and two conditions by BioinfGuru ★ 1.7k
Thanks @lchart, I accept that, I'll wait to see if there's any more who want to input and then accept the answer
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