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Yes, definitely lots of noise and few peaks for such an experiment. Which celltype is that, and which lab protocol did you use?
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What do you mean by gene lists?
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You can use e.g. the WGCNA package for network analysis. In your case multiple samples this might mean multiple patients or a time series o…
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Thank you so much for your responses.
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I think you could try doing this, but it wouldn't be appropriate. You'd be bringing long-read errors, which I believe are higher than short…
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I opened tow terminals and one was the code to build a decoy-aware transcriptome and the other was building indexes (no decoy)
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Thank you for the explanation! So just want to confirm one control with replicate is totally useless even can't use as a reference?
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If the top $CPU column shows some values in the hundreds then it is doing something (actually when it's > 0%). So based on the screenshot i…
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I divided my contigs using R, but it was more complex than the program Pierre Lindenbaum made (https://jvarkit.readthedocs.io/en/latest/Vcf…
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Just wait until it is finished. It's seems to be running. I just checked my logs and on our HPC with four cores such as process for a decoy…
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