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1,000 results • Page
2 of 20
Sort: Rank
Rank
Views
Votes
Replies
0
votes
1
reply
166
views
Co-Expression of All Genes with a Single Gene (Spatial Transcriptomics)
Transcriptomics
Correlation
Seurat
scRNA-seq
Spatial-Transcriptomics
updated 4 days ago by
jv
★ 1.2k • written 5 days ago by
ivingan
• 0
0
votes
1
reply
184
views
How to find complete overlaps between between multiple .bed files with bedtools
bedtools
granges
updated 5 days ago by
Pierre Lindenbaum
154k • written 5 days ago by
Alewa
▴ 110
0
votes
2
replies
194
views
Don't see uploaded files for linking in EGA (European Genome Archive)
EGA
5 days ago by
optimistsso4co3
▴ 100
0
votes
0
replies
115
views
Tools for network enrichment analysis in bacteria
network-enrichment-analysis
5 days ago by
langziv
▴ 20
1
vote
2
replies
2.0k
views
Error in Adding 1000Genomes Ancestral Allele info: Using VCF tools fill-aa
vcftools
samtools
faidx
tabix
1000Genomes
updated 5 days ago by
barslmn
★ 1.5k • written 5.6 years ago by
shrutishreyajha
▴ 10
1
vote
3
replies
328
views
Variant calling analysis
vcf
variant-calling
wgs
updated 5 days ago by
Medhat
9.6k • written 6 days ago by
namck
• 0
0
votes
0
replies
161
views
Comparing gene expression with copy number variation in TCGA
BiomaRt
TCGABiolinks
6 days ago by
Will Yam
▴ 30
0
votes
0
replies
537
views
Generating variant read count matrix, total read count matrix and binary/ternary mutaion matrix for SNV from scDNAseq FASTQ files
SNV
scDNAseq
mutationMatrix
tumorPhylogney
mutaion
6 days ago by
supernovamik
• 0
0
votes
1
reply
250
views
Trimmomatic run error
Trimmomatic
adap
updated 6 days ago by
Pierre Lindenbaum
154k • written 6 days ago by
eshrakaali_p
• 0
1
vote
5
replies
487
views
kraken on nt library
kraken
nt
4 days ago by
Francois Piumi
▴ 30
3
votes
1
reply
252
views
chromosome location to gene name and aa change
variant-annotation
6 days ago by
Zi
• 0
0
votes
1
reply
243
views
DESeq2 : parameter of Negative Binomial model in a context of gene expression
rnaseq
DESeq2
NB
7 days ago by
Picasa
▴ 640
0
votes
1
reply
237
views
Are there any recommended tools for tumor target sequencing data downstream analysis
target
tumor
downstream
compare
plots
7 days ago by
alwayshope
• 0
0
votes
0
replies
186
views
CRISPR-DAV
knockout
indel
CRISPR
detection
CRISPR-DAV
7 days ago by
Sowmya
• 0
0
votes
2
replies
346
views
modelling time series data from different individuals
modeling
3 days ago by
Sara
▴ 220
0
votes
0
replies
189
views
Multiple plot are displayed on one plot by matplotlib
Rstudio
python
r
7 days ago by
ali.kian.saei
▴ 10
0
votes
0
replies
200
views
What is the node_id in vg giraffe output (gaf or gam)?
giraffe
vg
7 days ago by
Hendricks27
• 0
0
votes
1
reply
321
views
Reducing the size of raw sequencing data in fastq format by using a simplified quality score
fastq
compression
updated 6 days ago by
ATpoint
72k • written 7 days ago by
rls_08
▴ 40
1
vote
3
replies
345
views
how to do an "reverse" oncoplot in maftools
maftools
Rstudio
updated 4 days ago by
Ram
39k • written 8 days ago by
JULIA HELENA
• 0
0
votes
0
replies
178
views
How to do diferential expression analysis of 2 closely related species
Trinity
EdgeR
Diferential-Expression
updated 8 days ago by
Ram
39k • written 8 days ago by
Fernando
• 0
0
votes
0
replies
186
views
Error in running Abyss assembler for chicken genome assembly
abyss
assembly
genome
fasta
1 day ago by
Maliha
▴ 20
0
votes
0
replies
195
views
Principal component analysis using plotPCA of deepTools
plotpca
deeptools
pca
8 days ago by
Rajendra KC
▴ 20
0
votes
0
replies
165
views
CORUM server not working?
corum
protein
complexes
database
8 days ago by
marta
• 0
4
votes
6
replies
508
views
How to pass from DNA to AA fasta
genetics
updated 5 days ago by
Buffo
★ 2.2k • written 8 days ago by
Anderson Stiward
• 0
2
votes
0
replies
209
views
Reference request for getting the big picture of gene taxonomy
genes
textbook
8 days ago by
garbagelister
▴ 20
0
votes
0
replies
186
views
Correct tRNA genes nomenclature in plant chloroplast genomes
chloroplast
tRNA
genes
genome
8 days ago by
Denis
▴ 270
0
votes
0
replies
169
views
Perl debugging help - miRWoods
perl
miRNA
8 days ago by
Gill
• 0
2
votes
6
replies
516
views
how to make a venn plot with data frame in R
plot
R
Venn
6 days ago by
satva72
• 0
0
votes
0
replies
201
views
normalize.quantiles in preprocessCore package with data containing missing values
preprocessCore
missing
normalize.quantiles
value
8 days ago by
tkmt
• 0
0
votes
3
replies
337
views
Picard ribosome intervals list
Picard
8 days ago by
mropri
▴ 140
0
votes
0
replies
203
views
Significance of "orientation" flag in FilterMutectCalls output
filtermutectcalls
gatk
VCF
mutect2
SNP
9 days ago by
bt_cepo
▴ 20
7
votes
12
replies
811
views
Integrated genes from bulk RNA seq and ATAC seq
bulk-RNA
integrated
ATAC-seq
updated 2 days ago by
Sasha
▴ 180 • written 9 days ago by
Chris
▴ 100
6
votes
6
replies
526
views
On "wings" in volcano plots...
DEG
volcano
DGE
statistics
DE
updated 8 days ago by
ATpoint
72k • written 9 days ago by
kalavattam
▴ 120
0
votes
0
replies
191
views
Comparison between z-scores of protein and mRNA levels
expression
Statistics
9 days ago by
Camilo Andres
▴ 30
1
vote
1
reply
188
views
DESeq2 with multiple factors
DESeq2
updated 9 days ago by
jv
★ 1.2k • written 9 days ago by
Nelo
• 0
2
votes
2
replies
288
views
DESeq factor order
DESeq2
9 days ago by
Chris
▴ 10
2
votes
1
reply
249
views
scRNAseq (bioconductor) data to seurat object
bioconductor
seurat
scRNAseq
updated 9 days ago by
ATpoint
72k • written 9 days ago by
Alex
▴ 20
1
vote
2
replies
220
views
StringTie across datasets
novel
transcripts
stringtie
rna-seq
9 days ago by
Prangan
▴ 20
0
votes
0
replies
186
views
Metatranscriptomics de novo assembly pipeline how to annotate/quantify
SALMON
denovo
rna
RNAspades
metatranscriptomics
10 days ago by
Lily
• 0
3
votes
9
replies
490
views
Estimate sizes of repeats in a especific Gene
CGG
Fragile-X
10 days ago by
Rafael
▴ 10
0
votes
0
replies
168
views
selection of Gene Ontology terms manually
RNAseq
Gene-ontology
GOanalysis
Clusterprofiler
10 days ago by
harish
• 0
3
votes
4
replies
2.4k
views
Unable to install Autogrid4
autogrid
software-error
autogrid4
updated 10 days ago by
Ram
39k • written 2.2 years ago by
roybatty269
• 0
0
votes
1
reply
173
views
Best tool for cross plate contamination removal
WTA
Microbe
Sequencing
Assembly
updated 10 days ago by
GenoMax
129k • written 10 days ago by
godwin
▴ 30
0
votes
0
replies
112
views
Analyzing aCGH dataset using R Programming
aCGH
10 days ago by
KayChn
• 0
0
votes
0
replies
127
views
Orientation bias in variants called by mutect2
ctDNA
gatk
tumor
Mutect2
SNP
10 days ago by
bt_cepo
▴ 20
0
votes
0
replies
144
views
How to align Illumina FASTQ isolates to a conserved gene for phylogenetic analysis
fastq
align
illumina
phylogenetic
10 days ago by
doggie
• 0
0
votes
0
replies
105
views
How to load inidividual ADT and RNA data to the same AnnData structure
python
CITE-seq
Scanpy
updated 10 days ago by
Ram
39k • written 10 days ago by
Pouria.momayyezi
• 0
0
votes
2
replies
225
views
Mapping reads to a multifasta reference
multifasta
map
updated 10 days ago by
WouterDeCoster
47k • written 10 days ago by
Link
• 0
0
votes
0
replies
110
views
Is it valid to use UMI-based analysis pipelines for aptamer-based proteomics
UMI
aptamer
RNA-seq
proteomics
10 days ago by
Ali
• 0
0
votes
1
reply
220
views
hierarchical clustering heatmap with r
R
updated 10 days ago by
iraun
6.0k • written 10 days ago by
orzrzlyo
▴ 20
1,000 results • Page
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Recent Votes
Answer: IGV displays genomic coordinates in a 1-based system or 0-based?
RNAseq expression data log2 transformed has negative values.
Samtools: "Too Many Open Files"
Filtering bad quality probes in illumina microarray data
gene filtering for agilent microarray using Limma
A: Identification Of Significant Differences Between Phylogentic Trees Using Distan
A: How to filter vcf file on minimum genotype depth and quality for each sample
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Answer: Single cell chemistry
by
swbarnes2
13k
Are you sure these samples are 10x? How likey is it that the sequencing group messed up and ran the samples wrongly?
Comment: How to deal with duplicated gene IDs in TCGA RNA expression data?
by
Vincent Laufer
★ 2.8k
are they distinct transcript IDs but the same gene ID? can you provide any additional context?
Comment: SyntaxError (Perhaps you forgot a comma?) in snakefile when running FastQC.
by
Dhatri Badri
• 0
that didn't work either
Answer: Why are some WES VCFs larger than others?
by
Vincent Laufer
★ 2.8k
Hi @kermit and prash (and others)! Nice discussion. I wanted to offer some **biological rationales** in addition to the technical reasons …
Answer: SyntaxError (Perhaps you forgot a comma?) in snakefile when running FastQC.
by
WouterDeCoster
47k
There is no `:` after `rule fastqc`
Comment: SyntaxError (Perhaps you forgot a comma?) in snakefile when running FastQC.
by
Dhatri Badri
• 0
sorry that was a typo while i was copying my code! I still get the same error
Comment: too many zeros in 16S rRNA amplicon sequencing data
by
andres.firrincieli
3.3k
You have too many ASV (40k) and I think this is a problem related to the denoising (DADA2) of NovaSeq 6000 sequencing data. Maybe this post…
Answer: SyntaxError (Perhaps you forgot a comma?) in snakefile when running FastQC.
by
Pierre Lindenbaum
154k
> fastq.gz". what is the dot after `fastq.gz" ` ?
Comment: DownsampleSam
by
Pierre Lindenbaum
154k
1) I think this is just a warning 2) there must be another error displayed 3) look at samtools view --subsample 4) don't use loops but use…
Comment: ABySS genome assembler
by
npavliukovec
• 0
Hello, yes, I tried it, but I have to do my home work on university's remote computer, unfortunatelly I go an error, when I try to use abys…
Comment: desgin a primer for CDS
by
yuxiang
• 0
how can you know the primer is not bind to other gene site? how can you desgin an experiment? I use the FISH to identify the gene, how abo…
Comment: desgin a primer for CDS
by
yuxiang
• 0
sorry for my late reply, I am a student, my profossor ask me to dsgin a primer for the specific cDNA, I have solved now, thanks for your re…
Comment: vg: Mapping fewer reads to a genome graph than a linear reference
by
samuel.a.odonnell
▴ 470
In this case filtering for quality might help correct for this overattributing feature?
Comment: ABySS genome assembler
by
GenoMax
129k
Did you see this guidance from ABySS manual: https://github.com/bcgsc/abyss#optimizing-the-parameters-k-and-kc
Comment: Single cell chemistry
by
GenoMax
129k
> I did it with cellRanger count and it went fine with good results It is not clear if you are referring to some other samples that worked…
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