Latest
Open
Jobs
Tutorials
Tags
About
FAQ
Community
Planet
New Post
Log In
New Post
Latest
Open
News
Jobs
Tutorials
Forum
Tags
Planet
Users
Log In
Sign Up
About
Limit : this month
all time
today
this week
this month
this year
315 results • Page
1 of 7
Sort: replies
Rank
Views
Votes
Replies
2
votes
20
replies
1.5k
views
The number of SVs called by `vg call` is much smaller than the number of SVs in the VCF used to construct the graph
vg
16 days ago by
Maxine
▴ 30
0
votes
13
replies
474
views
STAR Intron Motif Script Gives Segmentation fault Error
STAR
Linux
2 hours ago by
Y
• 0
0
votes
11
replies
691
views
Normalization for microarrays >1000+ samples?
microarray
normalization
oligo
3 days ago by
evmae
• 0
2
votes
11
replies
1.4k
views
RepeatMasker error when trying to generate repeat sequence distribution pie chart (all code and errors provided)
repeatmasker
updated 23 days ago by
rfran010
▴ 790 • written 5 weeks ago by
epianalysis
• 0
0
votes
10
replies
682
views
Quantification after transcriptome assembly with Trinity
De-novo-transcriptome-assembly
Trinity
RNA-seq-analysis
23 days ago by
langziv
▴ 50
3
votes
10
replies
434
views
STAR index not working
STAR
RNA-Seq
1 day ago by
camillab.
▴ 130
1
vote
10
replies
821
views
Coverage histogram query
Alignment
29 days ago by
prasundutta87
▴ 660
1
vote
10
replies
875
views
Deepvariant variant calling by singularity
deepvariant
singularity
conda
docker
updated 12 hours ago by
jeffhsu3
• 0 • written 11 weeks ago by
fra.silvestro99
▴ 10
0
votes
8
replies
735
views
Chipseq analysis on repeat genes
galaxy
repeatmasker
ChIP-seq
repeat-elements
ATAC-seq
1 day ago by
pb11
▴ 10
5
votes
7
replies
314
views
Salmon index not progressing
salmon
updated 1 day ago by
Michael
53k • written 1 day ago by
camillab.
▴ 130
0
votes
7
replies
2.7k
views
qPCR: Huge variation in fold change of genes between biological replicates
qpcr
fold-change
updated 8 days ago by
Ram
40k • written 5.3 years ago by
AP
▴ 80
0
votes
7
replies
779
views
Mugsy error -directory must be a directory
Mugsy
updated 2 days ago by
Ram
40k • written 2.8 years ago by
A_heath
▴ 140
2
votes
7
replies
661
views
VG mapping paired-end reads: error [xg]: multiple hits for XXX
variation-graph
reads
pangenome
vg
paired-end
19 days ago by
nkls063408
• 0
0
votes
6
replies
281
views
genome data downloads for various strains
gisaid
hass-marr
updated 1 day ago by
Ram
40k • written 2 days ago by
iftikharmaryam123
• 0
0
votes
6
replies
842
views
FACS (Fluorescence-activated cell sorting NOT cyTOF),Automating the removal of dead and duplicated cells
FACS
2 days ago by
ccbb7aab4
▴ 20
2
votes
6
replies
2.4k
views
hisat2 :Killed (ERR): hisat2-align exited with value 137
RNA-seq
alignment
hisat2
updated 29 days ago by
Ram
40k • written 21 months ago by
iamsmor
• 0
2
votes
5
replies
428
views
Dowload data from password protected cloud storage
data-transfer
wget
rclone
updated 15 days ago by
Joe
21k • written 16 days ago by
Paula
▴ 60
3
votes
5
replies
377
views
Ensembl site unresponsive in clusterProfiler analyses
parallell
clusterProfiler
R
updated 16 days ago by
manaswwm
▴ 440 • written 17 days ago by
enanoide
• 0
3
votes
5
replies
991
views
Running purge_dups on a hybrid assembly (optical mapping + reads)
pacbio
purge_dups
Assembly
bionano
updated 9 days ago by
colindaven
5.5k • written 2.6 years ago by
pablo
▴ 280
8
votes
5
replies
1.6k
views
BLASTp command line : filtering results by a minimum % of identity and % coverage
BLASTp
updated 2 days ago by
Ram
40k • written 23 months ago by
A_heath
▴ 140
0
votes
5
replies
395
views
How to index genome file for MethylDackel?
MethylDackel
indexing
Genome
methylation
updated 24 days ago by
ATpoint
76k • written 25 days ago by
harshraje19
▴ 40
0
votes
5
replies
321
views
manhattan plot with vcf information
R
manhattan
vcf
updated 2 days ago by
dthorbur
▴ 550 • written 4 days ago by
sooni
▴ 10
4
votes
5
replies
893
views
tranfering sam file easy and fast way
SAM
RNA-seq
hisat2
updated 29 days ago by
Ram
40k • written 20 months ago by
iamsmor
• 0
0
votes
5
replies
442
views
Convert gene id's to gene symbol preserving gene id's in deseq2
ensembl
r
DE
deseq2
updated 7 days ago by
Ram
40k • written 8 days ago by
dylannicoembros
• 0
1
vote
5
replies
941
views
What statistical test to apply for DE after CibersortX deconvolution
cibersortx
differential-expression
t-test
deconvolution
11 days ago by
Sam
▴ 290
2
votes
5
replies
368
views
Centrality analysis to identify TFs
TF
updated 22 days ago by
biofalconch
▴ 960 • written 24 days ago by
Chris
▴ 180
3
votes
5
replies
503
views
Neither pysam nor samtools reading HTTPS-sourced BAM file correctly
bam
pysam
samtools
updated 20 days ago by
Istvan Albert
98k • written 21 days ago by
Alex Reynolds
35k
1
vote
5
replies
664
views
Mugsy percentage of identity
Mugsy
Genome-alignment
updated 2 days ago by
Ram
40k • written 2.5 years ago by
A_heath
▴ 140
4
votes
5
replies
467
views
Pre-processing for single cell RNAseq: Hard thresholds, data (cluster)-driven or both?
scRNA-seq
5 days ago by
psm
▴ 100
3
votes
5
replies
419
views
Optimal number of features to use when integrate multi samples by Seurat in scRNAseq
scRNASeq
Seurat
updated 21 days ago by
Ram
40k • written 22 days ago by
alwayshope
▴ 30
2
votes
5
replies
347
views
Cluster annotation in single cell
Single-cell
updated 16 days ago by
Ram
40k • written 17 days ago by
synat.keam
▴ 80
2
votes
5
replies
1.0k
views
Find right adapter sequence for trimming
fastqc
flexbar
RNA-seq
updated 29 days ago by
Ram
40k • written 21 months ago by
iamsmor
• 0
0
votes
5
replies
559
views
Bowtie2 with secondary alignment option produces multiple mapping with offset and varying alignment scores
bowtie2
score
alignment
multiple-alignment
19 days ago by
polag01
▴ 10
1
vote
5
replies
474
views
Idat raw data conversion
idat
updated 5 days ago by
Giulio Genovese
▴ 330 • written 7 days ago by
Zi
• 0
0
votes
4
replies
402
views
PolyA and PolyG sequences in FastQC/MultiQC report
multiqc
fastqc
updated 24 days ago by
Marco Pannone
▴ 760 • written 25 days ago by
kenneditodd
▴ 20
0
votes
4
replies
254
views
Analyzing time-patterns in RNA Seq data only having results_apeglm
R
sequencing
10 days ago by
Paul
• 0
2
votes
4
replies
357
views
join the control replicates
RNA-seq
updated 8 days ago by
seidel
11k • written 9 days ago by
Jean Pierre
• 0
0
votes
4
replies
355
views
Error using sequenza-utils with WES
WES
NGS
Sequenza
Sequenza-utils
22 days ago by
Camilo Andres
▴ 40
0
votes
4
replies
274
views
Split reads along the genome in my samples
DNA
sequencing
10 days ago by
paulaotero.sanchez
• 0
2
votes
4
replies
414
views
Help with celltype annotation
seurat
single-cell
6 days ago by
Chris
▴ 180
2
votes
4
replies
288
views
Refseq annotation | Populus trichocarpa
R
RNA-seq
plants
Refseq
updated 3 days ago by
Ram
40k • written 3 days ago by
P
• 0
0
votes
4
replies
343
views
BLAST severe RAM throttling issues on Linux (Ubuntu)
ubuntu
ram
blastx
linux
blast
updated 29 days ago by
Istvan Albert
98k • written 4 weeks ago by
Wilford203
• 0
1
vote
4
replies
376
views
How to understand if a GWAS sample comes from Chromosome X or Y
python
23 days ago by
dzisis1986
▴ 60
3
votes
4
replies
301
views
Visualization of multiple sequence alignment quality
blastp
blast
msa
updated 18 days ago by
GenoMax
134k • written 18 days ago by
dec986
▴ 370
1
vote
4
replies
266
views
rRNA filtering from human RNA-seq data
rRNA
RNA-seq
4 days ago by
deniz
▴ 10
3
votes
4
replies
411
views
During alignment STAR has stopped at started alignment step
STAR
updated 3 days ago by
Ram
40k • written 6 weeks ago by
Santhi
• 0
0
votes
4
replies
613
views
CAZy database has multiple family sequence...
metagenomics
cazy
annotation
updated 17 days ago by
JyiYeung
• 0 • written 9 months ago by
GYUDAE
• 0
1
vote
4
replies
297
views
Gene expression
RNA-seq
DESeq2
Heatmap
updated 17 days ago by
ATpoint
76k • written 17 days ago by
Ralph
• 0
3
votes
4
replies
838
views
Ballgown DE analysis
R
ballgown
Differential-expression
updated 29 days ago by
Ram
40k • written 20 months ago by
iamsmor
• 0
4
votes
4
replies
421
views
Feedback on mouse brain scRNA-seq quality control
mouse
brain
singe-cell
scRNAseq
quality-control
29 days ago by
nshenoy
▴ 50
315 results • Page
1 of 7
Recent Votes
Comment: Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempti
Comment: Error in bowtie2
Recommended Tools For Alternative Splicing Detection From Rna-Seq Data
Comment: Using STAR aligner to build index of hg38
Answer: How to sort using samtools
The Biostar Handbook. A bioinformatics e-book for beginners.
The Biostar Handbook. A bioinformatics e-book for beginners.
Recent Locations •
All
ETH Zurich,
2 minutes ago
Sweden,
7 minutes ago
Netherlands,
13 minutes ago
Canada,
20 minutes ago
Denmark,
21 minutes ago
Cambridge, MA,
24 minutes ago
Canada,
24 minutes ago
Recent Awards •
All
Popular Question
to
langziv
▴ 50
Popular Question
to
Emily
▴ 30
Popular Question
to
Bogdan
★ 1.4k
Popular Question
to
paria
▴ 70
Popular Question
to
Ahmed.waraky
▴ 10
Teacher
to
fracarb8
★ 1.2k
Scholar
to
antonioggsousa
2.8k
Recent Replies
Comment: Hugo_Symbol to Entrez ID
by
shakyaram079
• 0
Yes sure. These are all gene IDs BZRAP1, C19orf60, TCEB3 and so on.
Comment: Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempti
by
Y
• 0
I tried the commands you recommended in the bash/command line. When I tried `find "${bam_directory}" -type f -name "*.bam" | xargs samtools…
Comment: Hugo_Symbol to Entrez ID
by
GenoMax
134k
Can you provide some examples of HUGO ID's you are unable to convert?
Comment: Using STAR aligner to build index of hg38
by
GenoMax
134k
GTF file includes gene models/annotation information. You could also use a program like `salmon/kallisto` with just human transcriptome to…
Comment: Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempti
by
Pierre Lindenbaum
157k
then test each files. find "${bam_directory}" -type f -name "*.bam" | xargs samtools quickcheck file "${reference_gen…
Answer: Ties in reranked list
by
alserg
▴ 840
The problem here is not the ties, but that your gene IDs in the pathway list (ensemble human genes) does not match the names of the stats v…
Comment: Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempti
by
Y
• 0
I did not use the space between the `-u` flag and the `-o` flag (the blank line) but I did do what you recommended: # Run CuffDiff wit…
Answer: Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempti
by
Pierre Lindenbaum
157k
how about simply trying: ``` cuffdiff -u "${annotation_gtf}" \ -o "${output_directory}" \ -b "${reference_genome}" \ -p "${num_th…
Comment: Using STAR aligner to build index of hg38
by
Grace
• 0
Yes, I know also need a GTF file. So the GTF file will include RNA information? Thanks for your answer!!
Comment: STAR Intron Motif Script Gives Segmentation fault Error
by
Y
• 0
I will try and figure it out on my own given what you all have mentioned. Thank you for your time.
Comment: Using STAR aligner to build index of hg38
by
Amitm
★ 2.2k
Have you gone through the manual [here][1]. In addition to the genome fasta file, you would also need a GTF file (of gene annotations) to b…
Comment: Highly inflated p-values in GWAS by regenie
by
4galaxy77
2.8k
You still definitely need to include principle components as covariates, even if your data is from the same ethnicity. Not including those …
Answer: How to create structural variants ground truth for alignment of two long-read ge
by
Christophe
• 0
Hi, D-genies is using minimap2 to align both genomes and minimap2 is chaining local alignments to produce a global one. If the SV are sm…
Comment: Ties in reranked list
by
Hamza
• 0
In this case there are no results in my fgsea object unfortunately
Comment: Genes with promoter and enhancer regions as GTF
by
abis.1819104
• 0
how to generate promoters.bed and enhancer.bed files from gtf file
Traffic: 2021 users visited in the last hour
Content
Search
Users
Tags
Badges
Help
About
FAQ
Access
RSS
API
Stats
Use of this site constitutes acceptance of our
User Agreement and Privacy Policy
.
Powered by the
version 2.3.6