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30 results • Page
1 of 1
Sort: Votes
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Views
Votes
Replies
8
votes
7
replies
171
views
Why does Ensembl VEP provide HGVSg for some variants and not others?
vcf
VEP
15 hours ago by
Jeremy Leipzig
21k
7
votes
8
replies
3.2k
views
DEseq2 design matrix with 3 factors
RNA-seq
deseq2
updated 13 hours ago by
Ram
39k • written 3.0 years ago by
kand3e
▴ 60
5
votes
8
replies
258
views
conda: command not found: gatk.
conda
gatk
17 hours ago by
bestone
▴ 20
2
votes
1
reply
1.1k
views
How to find the annotation file for GPL16956 platform of Arraystar human lncRNA microarray V3 (Probe Name Version)?
annotation
lncRNA
microarray
probe
updated 13 hours ago by
Ram
39k • written 3.0 years ago by
daphneolivia39
▴ 20
2
votes
2
replies
397
views
General Tips On Using Optimal Alignment Flags For Specific Data
bowtie2
alignment
bwa
updated 17 hours ago by
Ram
39k • written 3.1 years ago by
blake.mcmurray
• 0
1
vote
2
replies
312
views
If I execute "AddOrReplaceReadGroups" on a sorted and duplicate-marked .bam file, do I have to re-sort and re-mark duplicates?
picard
AddOrReplaceReadGroups
bam
read-groups
updated 18 hours ago by
Ram
39k • written 1 day ago by
javiflaja
• 0
1
vote
1
reply
1.9k
views
RStudio Cloud : Problem in Package Installing
RStudio
Bioconductor
updated 14 hours ago by
Ram
39k • written 3.1 years ago by
Rishabh Jha
• 0
1
vote
1
reply
112
views
Filtering content in heatmap to highest values
R
heatmap
ggplot2
7 hours ago by
cthangav
▴ 50
0
votes
0
replies
636
views
Advice on downstream analisys: data from RNA-Seq
differentially-expressed-genes
RNA-Seq
updated 17 hours ago by
Ram
39k • written 3.1 years ago by
marcelolaia
▴ 10
0
votes
0
replies
58
views
How does kallisto genomebam handle multi mapping reads?
kallisto
updated 15 hours ago by
Ram
39k • written 16 hours ago by
bioinfo
▴ 80
0
votes
3
replies
1.0k
views
Translate Multiple Sequences With Bio.Seq
sequence
python
updated 14 hours ago by
Ram
39k • written 3.1 years ago by
rjhowanski
• 0
0
votes
0
replies
441
views
Online Sequence Alignment of COVID-19 and Other Viruses (H1N1, Ebola, ect)
Sequence
updated 14 hours ago by
Ram
39k • written 3.1 years ago by
gtasource
▴ 60
0
votes
4
replies
498
views
exome seq- Post mapping question
variant-calling
exome-seq
updated 14 hours ago by
Ram
39k • written 3.1 years ago by
geneart$$
▴ 50
0
votes
0
replies
98
views
Using Tumor Immune Dysfunction and Exclusion (TIDE)
TPM
cancer
r
13 hours ago by
JACKY
▴ 100
0
votes
1
reply
493
views
Prediction of lnRNA interaction with all posible miRNA in Humans.
lnRNA
miRNA
RNA-Seq
RNA
updated 13 hours ago by
Ram
39k • written 3.0 years ago by
Talarash0
• 0
0
votes
4
replies
1.7k
views
HOMER load genomes not working
HOMER
rna
pombe
genome
updated 12 hours ago by
Ram
39k • written 2.9 years ago by
c78483
• 0
0
votes
2
replies
113
views
How to deal with SRA file without .SRA
SRA
9 hours ago by
Andy
▴ 50
0
votes
0
replies
40
views
Why in Heatmap plot my case and control samples of single cell dataset are not well clustered?
cell
single
DESeq2
Heatmap
6 hours ago by
paria
▴ 70
0
votes
4
replies
197
views
CellRanger problem
RNA-seq
STAR
scRNA
5 hours ago by
Carlos Eduardo
• 0
0
votes
2
replies
186
views
Where to get the molecular subtype information of STAD sample in TCGA
TCGA
database
2 hours ago by
younglin113
▴ 50
0
votes
6
replies
382
views
Problem with GPU guppy_basecaller and SLURM
nanopore
guppy
2 hours ago by
kenneditodd
▴ 10
0
votes
0
replies
67
views
cannot find genes with abricate and VFDB
abricate
wgs
vfdb
22 hours ago by
luciano.g
• 0
0
votes
6
replies
107
views
GISTIC from ASCAT
alteration
copy-number
sequencing
1 hour ago by
Hyper_Odin
▴ 280
0
votes
2
replies
823
views
Can I send my PyMol results to a web-page as a result for a submitted query? If not, how can I get a 3d render of a protein as a result for a user su…
protein-structure
pymol
protein
pdb
python
21 hours ago by
Aatif Hashmi
• 0
0
votes
0
replies
65
views
how to ensure that embedding for the new data is initialized based on the embedding of the existing data in UMAP?
UMAP
21 hours ago by
Raheleh
▴ 250
0
votes
0
replies
371
views
Calculate unique number of transcripts for each sample
R
RNA-Seq
updated 19 hours ago by
Ram
39k • written 3.2 years ago by
hannah.flintham
• 0
0
votes
0
replies
104
views
Changing gap open and extension penalties in muscle (or any other command line tool that works on mac, really)
multiple-sequence-alignment
updated 18 hours ago by
Ram
39k • written 1 day ago by
sodiumnitrate
▴ 20
0
votes
0
replies
56
views
using non-matched read counts for differential expression analysis (ncRNA)?
sRNAbench
RNA-seq
expression-matrix
sRNAtoolbox
updated 17 hours ago by
Ram
39k • written 18 hours ago by
danazeid3
• 0
0
votes
4
replies
1.6k
views
RNAseq analysis: t-test ?
RNA-seq
updated 17 hours ago by
Ram
39k • written 3.1 years ago by
Novice
• 0
0
votes
0
replies
912
views
PSORTb standalone version does not work...
Subcellular-localization
PSORT
updated 17 hours ago by
Ram
39k • written 3.1 years ago by
mbmember
• 0
30 results • Page
1 of 1
Recent Votes
Differences between trimming tools: cutadapt, bbduk and sickle
C: How sickle (adaptive trimming tool) work.
How sickle (adaptive trimming tool) work.
Comment: Help with running ATAC using Encode pipeline
Answer: Is there a file format better suited for the era of pangenomics than the .vcf? W
Answer: Is there a file format better suited for the era of pangenomics than the .vcf? W
A: Can EdgeR of DeSeq be used for Single-cell RNA-seq?
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Popular Question
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jobs
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Recent Replies
Comment: GISTIC from ASCAT
by
Hyper_Odin
▴ 280
Also, I would like to add that we cannot use ASCAT output for GISTIC input...since GISTIC expects all the chromosomes in the input file eve…
Comment: GISTIC from ASCAT
by
Hyper_Odin
▴ 280
Hi, Ram thanks for the help...I figured out the issue. somehow I had duplicate files in the folder which caused the issue.
Answer: multiqc: group samples by population + boxplot ?
by
Phil Ewels
★ 1.2k
You can't group and make boxplots as you describe, probably your best bet is to use the [highlight tool][1] in the report (especially if yo…
Comment: Problem with GPU guppy_basecaller and SLURM
by
kenneditodd
▴ 10
Nope - but I just noticed another error message in the guppy log. I've seen some people have the same problem on nanopore community but not…
Comment: Where to get the molecular subtype information of STAD sample in TCGA
by
younglin113
▴ 50
Thanks a lot! But still a bit curious why this information is not included in GDC or UCSC xena.
Comment: CellRanger problem
by
Carlos Eduardo
• 0
I'm using cellranger mkref --genome=Viral --fasta=path.fna --genes=path.gtf I have 126G RAM
Comment: How do I run DE for TPM values (not CPM)?
by
John
• 0
Thanks, but it's giving me the error that expression object should be numeric and that there are 2 non-numeric columns (because the first t…
Comment: How do I run DE for TPM values (not CPM)?
by
LChart
2.7k
you're mixing two packages, edgeR ("dgelist") and limma ("lmFit"). Just put your log(1+TPM) matrix as the first argument to lmFit.
Answer: hisat2-align exited with value 1
by
GenoMax
130k
You only need to use the `basename` of the index when you specify it. Try hisat2 -p 4 -x /home/ngs/zebra_fish/zebra_fish -U SRR10480…
Comment: How to deal with SRA file without .SRA
by
Andy
▴ 50
Thank you Ram! Yes, I did something wrong.
Comment: Is there a file format better suited for the era of pangenomics than the .vcf? W
by
Ram
39k
Exactly. Even when we were trying to leverage hail at my previous job, we ran into troubles constantly with data freezes. IMO academia is n…
Comment: Is there a file format better suited for the era of pangenomics than the .vcf? W
by
Jeremy Leipzig
21k
OK hospitals in particular definitely don't want to regenerate anything as individual new samples are added to the variant store. Even the …
Comment: How to deal with SRA file without .SRA
by
Ram
39k
PRJNA is the standard NCBI BioProject prefix. There are 19 pairs of FASTQs associated with this one so you're doing something wrong
Comment: Is there a file format better suited for the era of pangenomics than the .vcf? W
by
Ram
39k
> But you can't live out of your suitcase forever. I'd compare it to a local department store vs Walmart - I think cloud/distributed stora…
Comment: How do I run DE for TPM values (not CPM)?
by
John
• 0
That's what I'm trying to do but again, I'm getting an error on the last line and I'm asking how to fix it.
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