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32 results • Page
1 of 1
Sort: Votes
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Votes
Replies
2
votes
2
replies
63
views
Gene reads all zero for each sample
R
salmon
DE
DESeq2
3 hours ago by
DYLAN NICO
• 0
2
votes
2
replies
90
views
Volcano Plot Output Inquiry: Graphs Facing Down
rstudio
volcano-plot
updated 3 hours ago by
dariober
14k • written 9 hours ago by
Pumla
• 0
2
votes
10
replies
704
views
Only one read per run - Trying to use CellRangerv7
Fastq
CellRanger
updated 5 hours ago by
Ram
43k • written 5 weeks ago by
Sky
▴ 10
1
vote
11
replies
255
views
struggle to get fasta files from ucsc goldenPath
ucsc
getfasta
fasta
updated 2 hours ago by
GenoMax
140k • written 8 hours ago by
Lila M
★ 1.2k
1
vote
12
replies
533
views
Nanopore data filtering using fastp
fastp
nanopore
4 hours ago by
emilydolivo97
• 0
1
vote
2
replies
95
views
Flow Cytometry Data Analysis by Seurat
Seurat
updated 3 hours ago by
Ram
43k • written 8 hours ago by
gdfsnkfns
• 0
0
votes
0
replies
58
views
meta-analysis on RNA seq AND microarray results
microarray
RNA
10 hours ago by
liuyibin2005
• 0
0
votes
0
replies
51
views
Is there a good tool/reference for PBP and porin analysis
PBP
porin
10 hours ago by
blur
▴ 280
0
votes
8
replies
413
views
STAR aligner options
STAR
9 hours ago by
theophile
• 0
0
votes
0
replies
53
views
Combine rename and rmdup in SeqKit to remove duplicate sequences and append N in header? Sort by occurence?
seqkit
fasta
9 hours ago by
Broccoli
• 0
0
votes
4
replies
243
views
Coverage observed in introns in the Knockdown genes but not in knockout genes
RNA-seq
STAR
IGV
8 hours ago by
rohitsatyam102
▴ 840
0
votes
9
replies
454
views
Nextflow ERROR : Timeout waiting for connection from pool
nextflow
updated 7 hours ago by
GenoMax
140k • written 6 days ago by
dzisis1986
▴ 70
0
votes
0
replies
71
views
Training
chip-seq
ScATAC-seq
7 hours ago by
Manhezz
• 0
0
votes
0
replies
48
views
SPOTlight deconvolution logFC, logFC cohen or mean.AUC
spatial-transcriptomics
SPOTlight
logFC
deconvolution
updated 5 hours ago by
Ram
43k • written 11 hours ago by
M'zelle
• 0
0
votes
1
reply
67
views
(sc)RNA-seq dataset for these cell lines: HEK293, HeLa, PC3 and U2OS
scRNA-seq
updated 5 hours ago by
Ram
43k • written 5 hours ago by
chiditatawmbi
• 0
0
votes
0
replies
33
views
Illumina Dna Prep kit for sequencing phage linear genomes
tagmentation
transposons
phagetermini
sequencing
4 hours ago by
alenew.am
• 0
0
votes
0
replies
39
views
Contradictions in GSEA results
GSEA
Enrichment-analysis
PEA
updated 4 hours ago by
Ram
43k • written 4 hours ago by
Tolga
▴ 20
0
votes
1
reply
90
views
Normalization strategies for comparing mapped reads across samples in RNA-seq
normalization
rnaseq
updated 4 hours ago by
Ram
43k • written 6 hours ago by
Picasa
▴ 640
0
votes
3
replies
242
views
FindAllMarkers not working (Error (data layers are not joined. Please run JoinLayersWarning: When testing 1 versus all)
FindAllMarkers
seurat
re-clustering
4 hours ago by
Nitin
• 0
0
votes
0
replies
53
views
How to change alternate conformation occupancy ?
Bio.PDB.Atom
Bio.PDB
biopython
updated 4 hours ago by
Ram
43k • written 6 hours ago by
udakshi
• 0
0
votes
5
replies
303
views
Parsing fasta file by coordinates
linux
fasta
updated 2 hours ago by
GenoMax
140k • written 2 days ago by
sorrymouse
▴ 120
0
votes
1
reply
49
views
Number of CPUs to use by DRAP for transcriptomic assmbly
DRAP
CPU
transcriptomics
updated 2 hours ago by
GenoMax
140k • written 4 hours ago by
Mohamed Abderrahmane
▴ 10
0
votes
1
reply
28
views
Looking for RPIP Illumina kit probe sequences
Sequences
Illumina
updated 2 hours ago by
GenoMax
140k • written 2 hours ago by
David Tachiquín
• 0
0
votes
0
replies
21
views
Is RNAseq data from a monoclonal cell line technically considered scRNAseq?
scRNA
RNAseq
1 hour ago by
sithlordashley
• 0
0
votes
1
reply
74
views
Merging featureCounts output for different species
featureCounts
RNA-seq
updated 1 hour ago by
Ram
43k • written 9 hours ago by
Christian
• 0
0
votes
2
replies
163
views
Why counting by diffbind and featurecounts differ?
featurecounts
diffbind
21 hours ago by
Ankit
▴ 500
0
votes
7
replies
75
views
Low number of both surviving reads after trimming
trimmomatic
trimming
updated just now by
Ram
43k • written 3 hours ago by
Jay
• 0
0
votes
7
replies
315
views
Salmon Quantification in Alignment based-mode
Salmon
RNA-seq
TPM
updated 20 hours ago by
GenoMax
140k • written 1 day ago by
Patadu94
• 0
0
votes
0
replies
81
views
doing some ontology enrichment analysis
GO
20 hours ago by
aleksk779
• 0
0
votes
2
replies
154
views
novel and know mir156 and mir172
a
updated 19 hours ago by
gayachit
▴ 200 • written 23 hours ago by
Fadmo
• 0
0
votes
0
replies
67
views
How to check if a few pathways have relationship?
MSigdb
REACTOME
KEGG
17 hours ago by
Chris
▴ 260
0
votes
0
replies
66
views
In scRNA, How should I set QC standards?
scRNA
QC
13 hours ago by
mnx0723
• 0
32 results • Page
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Recent Votes
Comment: F*up Night style events for Bioinformatics ? Comment if you're interested!
Answer: F*up Night style events for Bioinformatics ? Comment if you're interested!
Answer: F*up Night style events for Bioinformatics ? Comment if you're interested!
Answer: F*up Night style events for Bioinformatics ? Comment if you're interested!
F*up Night style events for Bioinformatics ? Comment if you're interested!
Comment: F*up Night style events for Bioinformatics ? Comment if you're interested!
Answer: F*up Night style events for Bioinformatics ? Comment if you're interested!
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Recent Replies
Answer: F*up Night style events for Bioinformatics ? Comment if you're interested!
by
Mensur Dlakic
★ 26k
I was trying to avoid re-living traumatic events, but here goes. This is an attempt to delete all the files with a certain extension but in…
Comment: Low number of both surviving reads after trimming
by
Jay
• 0
Thank you for your response. There was no particular reason of `LEADING:20 and TRAILING:20`, but I used the same parameters as I had used …
Comment: Low number of both surviving reads after trimming
by
GenoMax
140k
A significant fraction of your reads appear to have the nextera sequence, that combined to this directive `LEADING:20` (do you have a reaso…
Comment: Low number of both surviving reads after trimming
by
Jay
• 0
Here is my fastqc, especially adapter content graph, before trimming(R1 and R2): ![enter image description here][1] And this after trimm…
Comment: Low number of both surviving reads after trimming
by
Jay
• 0
Thanks, I modified.
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160k
11 years ago: https://www.biostars.org/p/7126/
Comment: F*up Night style events for Bioinformatics ? Comment if you're interested!
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Dave Carlson
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haha yes exactly! After the first or second ruined fasta, I started "catting" all fasta files and piping to other commands, just to reduce …
Comment: F*up Night style events for Bioinformatics ? Comment if you're interested!
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Istvan Albert
100k
... ha, beat me to it, posted at the same time ... this error rules them all .. here is a cool trick on how to count fasta headers in file…
Answer: F*up Night style events for Bioinformatics ? Comment if you're interested!
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I've definitely ruined some genome fasta files by running `grep ^> mygenome.fasta` instead of `grep "^>" mygenome.fasta`
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o.nad
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Hello. I faced the same problem but not able to resolve it. I don't have double quotation and no NA values and the dataset used is in the f…
Answer: Annotation of Complex genomes using NCBI Genome Workbench
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Hi Recently, I used https://www.gensas.org/ to make the annotation. It was pretty practical and in my opinion easy. You just need to up…
Comment: Hybrid assembly Pacbio- Nanopore
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Lissa Cruz Saavedra
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Hi Thanks for your answer. I think I have very good coverage. My genome size is 48M. I also have data from poreC and illumina, the last on…
Comment: Variant calling of GBS data
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Anyone please suggest which variant caller can be the best choice for variant calling of GBS data for polyploid plant.
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More than likely they will only be available in the accompanying analysis app (Explify RPIP) in BaseSpace. Check with Illumina tech support…
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