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84 results • Page
2 of 2
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0
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3
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705
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to annotate BEDPE files
bedtools
annotate
updated 3 hours ago by
Lhl
▴ 760 • written 2.6 years ago by
Bogdan
★ 1.4k
0
votes
1
reply
95
views
Calculating haplotype and nucleotide diversity with deep amplicon sequencing data
haplotype
diversity
Pegas
Arlequin
HTS_data
2 hours ago by
deorugz
• 0
0
votes
2
replies
115
views
Troubles launch IGV on Linux(Debian)
IGV
updated 1 hour ago by
barslmn
★ 1.8k • written 16 hours ago by
PoscaXVIII
• 0
0
votes
3
replies
184
views
How to determine the total count for each gene in lymphotype B
scRNAseq
Seurat
updated 27 minutes ago by
fracarb8
★ 1.2k • written 1 day ago by
dalibenam64
• 0
0
votes
0
replies
6
views
Detect STRs in illumina library
repeats
annotation
STR
10 minutes ago by
kirillkirilenko
▴ 20
0
votes
4
replies
84
views
Sequence quality drops in the 3' end of the left sequence in paired end. What could be the cause?
sequencing
updated just now by
GenoMax
134k • written 3 hours ago by
eggrandio
▴ 40
0
votes
0
replies
118
views
Any way to predict hormone biosynthesis pathways in understudied organisms?
genomics
orthologs
biosynthesis-pathway
updated 6 days ago by
Ram
40k • written 7 days ago by
Mark
• 0
0
votes
1
reply
164
views
Prank checkpoint and restore?
prank
alignment
updated 6 days ago by
Ram
40k • written 6 days ago by
Pit
• 0
0
votes
2
replies
235
views
Is it okay to just average the log2FC values across different cell types in pseudobulk scRNA-seq data?
scrna-seq
rna-seq
log2fc
6 days ago by
JLee
• 0
0
votes
11
replies
731
views
Normalization for microarrays >1000+ samples?
microarray
normalization
oligo
6 days ago by
evmae
• 0
0
votes
0
replies
181
views
Illumina methylation EPIC V2 array
ewas
methylation
enrichment
missMethyl
EPIC
5 days ago by
juliviglino
• 0
0
votes
5
replies
357
views
manhattan plot with vcf information
R
manhattan
vcf
updated 5 days ago by
dthorbur
▴ 550 • written 8 days ago by
sooni
▴ 10
0
votes
1
reply
202
views
Esearch, Epost, and Efetch for Large Datasets in Biopython
eutils
biopython
entrez
updated 5 days ago by
Ram
40k • written 6 days ago by
Salem
• 0
0
votes
1
reply
381
views
Close genome comparison
Mauve
Mugsy
genome-comparison
GSAlign
updated 5 days ago by
Ram
40k • written 19 months ago by
A_heath
▴ 140
0
votes
0
replies
362
views
Distinction of two very close bacterial strains
mutations
bacteria
updated 5 days ago by
Ram
40k • written 2.3 years ago by
A_heath
▴ 140
0
votes
3
replies
731
views
How to add translation of CDS in Artemis?
translation
Artemis
updated 5 days ago by
Ram
40k • written 2.7 years ago by
A_heath
▴ 140
0
votes
1
reply
757
views
How to identify specific region using Mauve?
Mauve
primers
updated 5 days ago by
Ram
40k • written 2.8 years ago by
A_heath
▴ 140
0
votes
7
replies
786
views
Mugsy error -directory must be a directory
Mugsy
updated 5 days ago by
Ram
40k • written 2.8 years ago by
A_heath
▴ 140
0
votes
1
reply
904
views
Issue interpreting plasmidSPAdes output
plasmidSPAdes
updated 5 days ago by
Ram
40k • written 2.9 years ago by
A_heath
▴ 140
0
votes
0
replies
595
views
Contig extension using PRICE
price
updated 5 days ago by
Ram
40k • written 3.0 years ago by
A_heath
▴ 140
0
votes
1
reply
217
views
Significance testing of top vs. random SNPs
SNPs
significance
variance
statistical
updated 5 days ago by
dthorbur
▴ 550 • written 9 days ago by
am29
▴ 30
0
votes
1
reply
209
views
How to download genomes and proteins from JGI in bulk via the command line?
jgi
cli
updated 5 days ago by
Ram
40k • written 5 days ago by
O.rka
▴ 680
0
votes
1
reply
199
views
OMA in AWS cloud
OMA
AWS
updated 5 days ago by
Ram
40k • written 5 days ago by
Ksel
• 0
0
votes
0
replies
171
views
High pvalues when using clusterProfiler for seurat
clusterProfiler
5 days ago by
Ahmed
• 0
0
votes
0
replies
163
views
scanpy problem for empty cells
scanpy
updated 5 days ago by
Ram
40k • written 5 days ago by
Andy
▴ 90
0
votes
0
replies
339
views
Why dbConnect GEOmetadb_demo.sqlite shows some information while dbConnect GEOmetadb.sqlite shows no information
R
GEO
GEOmetadb
updated 4 days ago by
Pierre Lindenbaum
157k • written 5 days ago by
nonaddldy
▴ 10
0
votes
1
reply
208
views
High amount of intronic/intergenic reads in SMARTer stranded total bulk RNAseq
RNA-seq
DNA
SMARTer
updated 4 days ago by
Ram
40k • written 4 days ago by
Mat
▴ 60
0
votes
0
replies
155
views
Compare peaks between clusters in sc-ATAC
Peak-Calling
Epigenome
scATAC-seq
updated 4 days ago by
Ram
40k • written 4 days ago by
Ahmed.waraky
▴ 10
0
votes
0
replies
139
views
My rMATS output file is not showing gene symbols and only XLOC gene ID are being shown.
rMATS
rMATS-turbo
3 days ago by
Nipan
• 0
0
votes
13
replies
563
views
STAR Intron Motif Script Gives Segmentation fault Error
STAR
Linux
3 days ago by
Y
• 0
0
votes
0
replies
152
views
Convert RNASeq V2 data in cBioPortal having RSEM normalized results to TPM values
bioinformatics
TPM
RNASeqV2
cbioportal
RSEM
updated 3 days ago by
4galaxy77
2.8k • written 4 days ago by
shakyaram079
• 0
0
votes
2
replies
269
views
Modify the code to take most abundant reads from a cluster and process it.
cd-hit-est
clustering
3 days ago by
Mo
▴ 40
0
votes
4
replies
465
views
Weirdness in annotation (missing allele frequencies)
allele-frequency
gnomad
annovar
updated 3 days ago by
Jeremy Leipzig
21k • written 4 days ago by
Can Abdullah
• 0
0
votes
0
replies
169
views
Current landscape of approaches to scRNA-seq with nanopore sequencers?
scRNA-seq
nanopore
single-cell
2 days ago by
LauferVA
3.7k
84 results • Page
2 of 2
Recent Votes
Peak annotation with HOMER. Statistics with the annStats parameter
Answer: Fetch Fastq files directly for SRA data
Answer: Fetch Fastq files directly for SRA data
A: Using TargetScan on other species
Comment: Low Alignment rate
Comment: ncbi error report log for validate fastq issue
GEO data mining (IB) - Downloading Sequence Read Archive (SRA) raw data
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Comment: Sequence quality drops in the 3' end of the left sequence in paired end. What co
by
GenoMax
134k
This may be one of those things that just happens to be a one-time thing with whatever was going on with this run. Was this sample pooled w…
Comment: ncbi error report log for validate fastq issue
by
GenoMax
134k
Can you provide details of what you are doing and the commands you are using?
Comment: Fetch Fastq files directly for SRA data
by
GenoMax
134k
> fetch fastq files for SRA data hosted by NCBI from AWS data exchange Please provide an example of a SRA accession where you are encount…
Comment: How the first sequencing template removed in pair end sequencing
by
GenoMax
134k
Moving this to an answer since there is a link for a paper. Things could have changed as @rfran010 says but this may be the closest we will…
Comment: Sequence quality drops in the 3' end of the left sequence in paired end. What co
by
Asaf
10k
What machine? (Nextseq? Novaseq?) what library prep method?
Comment: How to determine the total count for each gene in lymphotype B
by
fracarb8
★ 1.2k
If you want the average expression, you should look at `AverageExpression` from seurat which returns the average instead of the sum. `Avera…
Answer: Fetch Fastq files directly for SRA data
by
Umer
▴ 50
You can also use **[Grabseqs][1]**. Easiest way is to use it with anaconda as **conda install -c louiejtaylor grabseqs** But you have…
Answer: chromosome location
by
Umer
▴ 50
What you can do is use **SnapGene** 1. Download the genome (fasta file) 2. Load it into Snapgene 3. Find gene locations based on the …
Comment: Troubles launch IGV on Linux(Debian)
by
barslmn
★ 1.8k
Your log seems fine. Could you add the results of these commands? - Which version of debian are you on? ``` lsb_release -c `…
Comment: How to plot coverage and depth statistics of a bam file
by
jl19
• 0
Hi William, I was trying to use bam2plot you have developed. It was installed on a python evnviroment python version 3.11. The installat…
Comment: Help writing code for a question on my homework
by
Joe
21k
This isn't really the right place for this question. Please ask this as a separate new question, but only if it is bioinformatics related.
Comment: How to determine the total count for each gene in lymphotype B
by
dalibenam64
• 0
thank you very much for your response I'm really grateful !! but I'm still confused because for example for patients I have 2 replicates…
Comment: Sequence quality drops in the 3' end of the left sequence in paired end. What co
by
eggrandio
▴ 40
But shouldn't quality drop at both 3'ends and not just on the left one?
Comment: Sequence quality drops in the 3' end of the left sequence in paired end. What co
by
ATpoint
77k
https://www.biostars.org/p/91624/
Comment: to annotate BEDPE files
by
Lhl
▴ 760
is this what you are looking for? https://github.com/imgag/ngs-bits/blob/master/doc/tools/BedpeGeneAnnotation.md
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