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152 results • Page
2 of 4
Sort: Votes
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Views
Votes
Replies
0
votes
0
replies
124
views
BIOM format can contain tabular data ?
BIOM
phyloseq
biomformat
R
2 days ago by
chemokine-1
▴ 10
0
votes
2
replies
211
views
Calculating percentage of cells proliferating in single cell rna seq data
statistics
scRNA-seq
proliferation
2 days ago by
mropri
▴ 150
0
votes
2
replies
212
views
VCF annotation with gnomADv4 using R package
annotation
gnomADv4
R
VCF
2 days ago by
DKA
▴ 40
0
votes
2
replies
184
views
Bedmethyl file format
bedmethyl
methylation
updated 2 days ago by
GenoMax
141k • written 2 days ago by
njornet
▴ 20
0
votes
1
reply
167
views
Annotating a dataset with gnomAD v4 using Hail
Python
Sequencing
Hail
Exome
Annotation
updated 2 days ago by
DKA
▴ 40 • written 2 days ago by
james.melhorn
• 0
0
votes
0
replies
269
views
Looking for an explanation as to what the Peak Score is from HOMER, not finding any answers on Biostars yet
Homer
ATAC-seq
2 days ago by
Ronin
• 0
0
votes
1
reply
157
views
Freyja plot error
Freyja
updated 1 day ago by
Ram
43k • written 2 days ago by
Adyasha
• 0
0
votes
0
replies
139
views
Struggling to lift genome builds
PLINK
liftover
GWAS
updated 1 day ago by
GenoMax
141k • written 1 day ago by
srimmer
• 0
0
votes
1
reply
1.2k
views
Controlling for batch effect in SNP-based PCA
whole-genome
PCA
batch-effect
SNP
updated 1 day ago by
Ram
43k • written 4.7 years ago by
Ace
▴ 90
0
votes
4
replies
1.8k
views
Correcting batch effect in unevenly distributed groups
RNA-Seq
batch-effect
R
updated 1 day ago by
Ram
43k • written 4.8 years ago by
jperezboza
▴ 20
0
votes
1
reply
825
views
How to compare differentially expressed genes among different affymatrix dataset
microarray
batch-effect
R
updated 1 day ago by
Ram
43k • written 4.8 years ago by
Calangoa
▴ 30
0
votes
0
replies
1.1k
views
Should batch correction be performed before or after feature selection in scRNA-seq
batch-effect
scRNA-seq
feature-selection
updated 1 day ago by
Ram
43k • written 4.9 years ago by
Lucy
▴ 140
0
votes
0
replies
1.7k
views
Batch effect and CCA
CCA
RNA-Seq
seurat
batch-effect
updated 1 day ago by
Ram
43k • written 5.1 years ago by
akivab2
▴ 110
0
votes
3
replies
1.8k
views
How to remove batch effect in copy number segment mean
Combat
CNA
Batch-Effect
updated 1 day ago by
Ram
43k • written 5.5 years ago by
sugus
▴ 150
0
votes
1
reply
1.3k
views
About BatchQC packages error
Batch-effect
R
updated 1 day ago by
Ram
43k • written 6.0 years ago by
Jun
• 0
0
votes
1
reply
2.8k
views
Can't make sva comparison despite very distinct batch effects
sva
batch-effect
updated 1 day ago by
Ram
43k • written 6.2 years ago by
jjrin
▴ 40
0
votes
1
reply
225
views
assembly using CCS, CLR, CCS_CLR sequences together?
assembly
updated 1 day ago by
GenoMax
141k • written 1 day ago by
ycts
• 0
0
votes
0
replies
132
views
filter the most significant ligand and receptors in netVisual_bubble in cell chat
cellchat
1 day ago by
synat.keam
▴ 100
0
votes
2
replies
318
views
How can I solve this error?
metal
1 day ago by
22211020193
• 0
0
votes
0
replies
130
views
Issues with SnpEff Assuming Circular Chromosomes in Eukaryotic Genome Analysis
snpEff
1 day ago by
ekirsch
• 0
0
votes
4
replies
430
views
How to reduce the variation of CIBERSORTx results?
CIBERSORTx
deconcolution
1 day ago by
feather-W
• 0
0
votes
0
replies
128
views
How to root an unrooted tree with a known root tree?
phylogenetics
rooting
R
1 day ago by
P.
• 0
0
votes
2
replies
285
views
GSEA analysis in R
GSEA
R
Arabidopsis
1 day ago by
Sudip
• 0
0
votes
1
reply
383
views
How to add rowLinks, rowTree in SummarizedExperiment manually.
phylogenetic-tree
microbiome
updated 1 day ago by
Ram
43k • written 19 months ago by
Muhammad
• 0
0
votes
0
replies
115
views
How can I obtain the tissue or sample name alongside the Tau score in tspex?
tau-score
tspex
updated 1 day ago by
Ram
43k • written 1 day ago by
bioinfo223
▴ 10
0
votes
0
replies
99
views
Pruning with plink finds a majority of SNPs in very high LD
LD
SNP
plink
pruning
1 day ago by
enferdeflame
• 0
0
votes
0
replies
106
views
traying to make a maf from an already annotated vcf
vcf2maf
VEP
maftools
updated 23 hours ago by
Ram
43k • written 23 hours ago by
Javier
• 0
0
votes
1
reply
182
views
QC exclusion of A/T or G/C alleles to avoid strand issues
quality-control
SNV
QC
SNP
updated 23 hours ago by
Ram
43k • written 23 hours ago by
_quantum_girl_
▴ 10
0
votes
0
replies
100
views
Variant Generation Using Longshot
long-reads
variant-calling
ONT
minion
longshot
updated 22 hours ago by
Ram
43k • written 22 hours ago by
samuelkalandarov2002
• 0
0
votes
1
reply
1.8k
views
Advice required for remove batch effect which perfectly collinear with contrast of interest
RNA-seq
batch-effect
updated 20 hours ago by
Ram
43k • written 6.6 years ago by
boymin2020
▴ 80
0
votes
0
replies
2.0k
views
NOISeq Batch Correction to RNA-seq data
Batch-effect
RNA-Seq
ARSyNseq
limma
NOISeq
updated 20 hours ago by
Ram
43k • written 6.9 years ago by
endikavarela
• 0
0
votes
2
replies
2.8k
views
RNA-seq unbalanced batch effect correction
limma
RNA-Seq
edgeR
batch-effect
updated 20 hours ago by
Ram
43k • written 7.0 years ago by
endikavarela
• 0
0
votes
0
replies
1.9k
views
Correcting batch effect from known variables in RnBeads using Combat
Combat
450k
methylation
RnBeads
batch-effect
updated 20 hours ago by
Ram
43k • written 7.1 years ago by
rahel14350
▴ 40
0
votes
0
replies
1.9k
views
batch effect in radseq
radseq
batch-effect
SNP
updated 20 hours ago by
Ram
43k • written 7.4 years ago by
oselm
▴ 50
0
votes
0
replies
726
views
Creating a stable batch-corrected scRNA-Seq data with multiple datasets and multiple integrations
RNA-Seq
scRNA-Seq
batch-effect
updated 20 hours ago by
Ram
43k • written 3.4 years ago by
hkarakurt
▴ 180
0
votes
1
reply
808
views
Bulk RNAseq MACS Sort Quality Contamination
EdgeR
Batch-effect
RNA-Seq
updated 20 hours ago by
Ram
43k • written 3.7 years ago by
jordan15
• 0
0
votes
0
replies
1.2k
views
Batch estimate using kBET
batch-effect
kbet
updated 20 hours ago by
Ram
43k • written 4.0 years ago by
getanid123
• 0
0
votes
3
replies
3.0k
views
Rna-seq for differential expression analysis
differential-expression
transcriptome
updated 20 hours ago by
Ram
43k • written 8.9 years ago by
siddharth.patel.153
• 0
0
votes
0
replies
1.7k
views
Differential expression using Scran batch corrected data
single-cell
Batch-effect
RNA-Seq
Scran
updated 20 hours ago by
Ram
43k • written 5.4 years ago by
baldissera152
▴ 10
0
votes
0
replies
108
views
Difference when using difference number of pathways score to compare between 3 groups
GSVA
Limma
19 hours ago by
Chris
▴ 260
0
votes
2
replies
517
views
autodockvina
python
python3
autodock
updated 18 hours ago by
Ram
43k • written 3 months ago by
iamsmor
• 0
0
votes
0
replies
93
views
create genewise sync file in popoolation
popoolation
updated 17 hours ago by
GenoMax
141k • written 19 hours ago by
N.Y.Wiyana-Hewage
• 0
0
votes
3
replies
356
views
RNA seq differential expression analysis
RNA-seq
normalization
updated 15 hours ago by
swbarnes2
14k • written 1 day ago by
rrehimi
• 0
0
votes
2
replies
372
views
How to get the reference panel for UKBB
UKBB
GWAS
clump
14 hours ago by
航太郎
• 0
0
votes
1
reply
268
views
Load a full GFF3 into annotation track using arrow (Apollo)
arrow
python-apollo
Apollo
updated 14 hours ago by
cmdcolin
★ 3.8k • written 1 day ago by
renan.igor
• 0
0
votes
2
replies
233
views
error in coding potential calculator CPC2
cpc
CPC2
coding-potential
11 hours ago by
Ashok
• 0
0
votes
2
replies
237
views
python file for coding potential calculator
cpc2.py
11 hours ago by
Ashok
• 0
0
votes
2
replies
253
views
miRNAs quantification using mirdeep2 tool
differential-expression
mirdeep2
miRNA
8 hours ago by
Ashok
• 0
0
votes
4
replies
476
views
Per base sequence content failed miserably
fastqc
sequence
trimmomatic
NGS
assembly
8 hours ago by
Kai Xin
• 0
0
votes
0
replies
67
views
Lncipedia GTF file error
Proteomics
Bioinformatics
7 hours ago by
atharvakarkare14
▴ 10
152 results • Page
2 of 4
Recent Votes
Answer: Problem with Sniffles pipeline and SV calling help
Illumina reads preprocessing best practice for snp calling applications
Comment: Illumina reads preprocessing best practice for snp calling applications
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How To Generate Phylogeny Tree, Based On Snps?
How To Construct Phylogenetic Tree Using Snps
Basic normalization, batch correction and visualization of RNA-seq data
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Comment: Illumina reads preprocessing best practice for snp calling applications
by
Enrique
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Hi! I'm still interested in the step that you use for the SNP calling. Did you use `trimmomatic` or another tool for trimming before mappin…
Comment: Could you please assist in identifying this cluster?
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The information do not suffice. Please show plots and identified marker genes. Also, make sure it is not a trash cluster with low number of…
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Thank you, I added 'accurate' now ;) I'm surprised to find out that there isn't some tool like multiqc that just summarizes all this infor…
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Guys, just use `samtools sort` which won't get you into trouble with any of that.
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I don't know for sure, but I think that decreasing the amount of threads (1 or 2 instead of 4) will decrease the memory requirements of the…
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For me, setting `TMP_DIR` (`--TMP_DIR /home/tmp`) didn't work in picard 2.21.1. What solved the issue was setting the environmental variabl…
Answer: Plink merge errors.
by
Dinmukhamed
• 0
I found answer https://www.biostars.org/p/101191/.
Comment: Ciriquant not configuring hisat2 indexed files
by
Atul K.
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it worked. Thank you. Now my terminal is crashing, seems 16gb of RAM and 4 cores isn't enough. Can you suggest something?
Comment: Adding CB tag to bam file
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Maria
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I checked Picard ```AddOrReplaceReadsGroups``` and ```AddOATag```. Is that what you're referring to?
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Noted that 'accurate' wasn't among your criteria ;) Anyway, most variant callers are free open-source software. However, for ancestry ana…
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> server so I cannot use Blat for this purpose. blat is avilable as a standane tool: http://hgdownload.soe.ucsc.edu/admin/exe/linux.x86…
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My favourite for this task would be [GMAP]( http://research-pub.gene.com/gmap ), which can produce GFF format with option `-f 2`. It is…
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thanks! This saved me quite some time, I was about to write code to calculate the aligned length from the CIGAR. But this can be done with …
Comment: Per base sequence content failed miserably
by
Kai Xin
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will look into that. Thank you very much
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