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Recent Replies
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Since the input file is named `normalized.csv` and the values look like floats most likely the counts have been already normalized somehow.
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https://www.ebi.ac.uk/ena/browser/view/PRJEB11202
https://www.ebi.ac.uk/ena/browser/view/PRJNA153427
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gRNAs are single-stranded and by this they have a strand-specificity (depends where the PAM is) and that gives them an orientation.
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You have commas as decimal delimiters which interpreted as text. Just replace by dot and make numeric:
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You can try:
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Was a tool ever found for this? I am curious.
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Great use case - we'll take a look. Thanks for trying it out!
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