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20 results • Page
1 of 1
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0
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5
views
How to solve this RoseTTAFold colaboratory error?
modeling
Google
Colaboratory
RoseTTAFold
protein
11 minutes ago by
benguyarenbeyaz98
• 0
0
votes
0
replies
11
views
How to calculate identity percentage between proteins contained in a FASTA file?
percentage
FASTA
alignment
identity
36 minutes ago by
v.berriosfarias
▴ 140
1
vote
8
replies
611
views
TCGA2STAT Error: Firehose connection
FIREHOSE
TCGA
updated 30 minutes ago by
Ram
43k • written 6 months ago by
Gnana
• 0
0
votes
6
replies
103
views
How do I use the STARSolo aligner with MGI DNBelab C series HT scRNAseq libraries?
snRNA-seq
STARSolo
scRNA-seq
STAR
1 hour ago by
atowns21
• 0
0
votes
1
reply
52
views
Find subcluster under a cluster, find differential genes in one cluster between 2 samples??
clustering
subclustering
scRNAseq
updated 2 hours ago by
bk11
★ 2.3k • written 3 hours ago by
alphaflylizard
• 0
0
votes
1
reply
60
views
How to trim transcripts using information from NCBI contamination screen report
RNAseq
assembly
transcriptome
contamination
updated 4 hours ago by
Pierre Lindenbaum
161k • written 4 hours ago by
Lada
▴ 30
0
votes
0
replies
37
views
The concept of co-occurrence network structure
co-occurence
microbiome
network
6 hours ago by
ohtang7
▴ 40
0
votes
1
reply
80
views
Plot DNA methylation level of DMR on the basis of certain genes
rrbs
methylkit
updated 8 hours ago by
GenoMax
141k • written 8 hours ago by
Srinka
▴ 20
0
votes
4
replies
167
views
Should I remove unlocalized scaffols in reference genome before alignment?
qualimap
alignment
minimap2
GRCh38
assembly
updated 6 hours ago by
GenoMax
141k • written 9 hours ago by
njornet
▴ 20
0
votes
0
replies
44
views
Software to calculate RSCU, ENC other than codonw
rscu
codonw
updated 6 hours ago by
Ram
43k • written 9 hours ago by
SHREYA
• 0
0
votes
0
replies
51
views
Merging replicates from Encode project
CHIP-seq
encode
10 hours ago by
Nurken
• 0
1
vote
3
replies
148
views
Filter BAM to keep only alignments at an interval, ignoring reads spliced over
samtools
bam
splicing
11 hours ago by
WouterDeCoster
47k
0
votes
2
replies
120
views
some error in building kraken2 database
metagenome
kraken2
updated 6 hours ago by
Ram
43k • written 11 hours ago by
Art1ess
• 0
0
votes
1
reply
83
views
Subset Seurat object from Xenium spatial data
Seurat
Xenium
Spatial
updated 6 hours ago by
bk11
★ 2.3k • written 12 hours ago by
Susmita Mandal
▴ 110
1
vote
1
reply
117
views
Failed kmer content
kmer
illumina
ngs
updated 6 hours ago by
Ram
43k • written 15 hours ago by
Kasturi
• 0
0
votes
1
reply
113
views
Heatmap and rna-seq
RNA-Seq
Heatmap
and
updated 13 hours ago by
Basti
★ 2.0k • written 16 hours ago by
qudrat.nii
▴ 10
0
votes
0
replies
53
views
How do I select a GC file (gc.wig)? (How to determine the inputdata of HMMcopy)
HMMcopy
Copy-number-analysis
R
updated 6 hours ago by
Ram
43k • written 17 hours ago by
SSSJec
• 0
0
votes
0
replies
76
views
File with NCBI summary or Uniprot description
annotation
visualisation
NCBI
Uniprot
RNA
updated 6 hours ago by
Ram
43k • written 1 day ago by
Amélie
• 0
0
votes
0
replies
66
views
Filtering most similarly expressed gene (contrary to differential expression gene) with NOISeq?
NOIseq
10 hours ago by
alifafiq1
• 0
2
votes
3
replies
176
views
interpretartion of a vcf file
calling
genotype
ONT
variant
diploid
updated 12 hours ago by
Pierre Lindenbaum
161k • written 1 day ago by
samuelkalandarov2002
▴ 10
20 results • Page
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Comment: Marking duplicates using UMIs
Comment: TCGA2STAT Error: Firehose connection
Answer: Is it Acceptable to Have Uniform Quality Scores in a FASTQ File?
Comment: Ideal PC configurations and operating system for bioinformatics laboratory
C: Threshold over percentage identity in blastn
A: Threshold over percentage identity in blastn
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v.berriosfarias
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• 0
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marco.barr
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141k
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▴ 120
Recent Replies
Comment: TCGA2STAT Error: Firehose connection
by
Ram
43k
If their github repo is alive, raise an issue there.
Comment: TCGA2STAT Error: Firehose connection
by
Ngrin
• 0
Yeah makes sense. I was following their github. Thanks for pointing this out.
Comment: The Biostar Herald for Tuesday, April 23, 2024
by
Pierre Lindenbaum
161k
bwa 0.7.18 released ! https://github.com/lh3/bwa/releases/tag/v0.7.18
Comment: Downsampling fastq file
by
Ram
43k
I'm moving this to a comment for now.
Answer: Seurat merge and batch correction
by
bk11
★ 2.3k
You can merge your seven Seurat objects and perform integration using **Seurat V5** to remove the batch effect- ```r library(Seurat) libra…
Comment: How do I use the STARSolo aligner with MGI DNBelab C series HT scRNAseq librarie
by
atowns21
• 0
Yeah, I've been digging around the web for a while now. Can't find any information about the barcodes. The total number of combinations is …
Comment: How do I use the STARSolo aligner with MGI DNBelab C series HT scRNAseq librarie
by
GenoMax
141k
Ah I see that in now. I am not sure why MGI has split the whitelist into two sections. You may need to see if you can dig up some info abou…
Comment: How do I use the STARSolo aligner with MGI DNBelab C series HT scRNAseq librarie
by
atowns21
• 0
read 1 is 30bp... which I think you meant to type. If you scroll approximately halfway down the json file I linked to, it has the following…
Comment: TCGA2STAT Error: Firehose connection
by
Ram
43k
The package has been removed from CRAN and BioConductor. Should you even be using it?
Comment: How do I use the STARSolo aligner with MGI DNBelab C series HT scRNAseq librarie
by
GenoMax
141k
The barcodes are in location 1-10 and you have the actual list there. I don't know for certain what is in position 11-20 (perhaps spacer, c…
Answer: Including plasmid in transcriptome assemblies
by
GenoMax
141k
> I want to use both STAR and BOWTIE2 for assembly Those programs are aligners not assemblers. But since you are working with bacteria yo…
Comment: How to slice a gvcf file with tabix?
by
Sd
• 0
I already have gvcfs called by chromosome and there are 24 gvcfs per sample which I merged them into a single gvcf per sample. Now I want …
Comment: Find subcluster under a cluster, find differential genes in one cluster between
by
bk11
★ 2.3k
First, could you please explain any reason for not using Seurat V5 approach for your data integration mentioned in [here][1]? > Now moving…
Comment: How do I use the STARSolo aligner with MGI DNBelab C series HT scRNAseq librarie
by
atowns21
• 0
So I'd take the json file located [here][1] and essentially create the whitelist to be all possible combinations of the position 1-10 barco…
Comment: TCGA2STAT Error: Firehose connection
by
Ngrin
• 0
Hey Gnana, could you find a way to solve the issue? I have the same error
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