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1,000 results • Page
1 of 20
Sort: replies
Rank
Views
Votes
Replies
41
votes
55
replies
12k
views
6 follow
hash function for DNA sequences
hash
hashing
DNA
8.1 years ago by
midox
▴ 290
4
votes
45
replies
7.7k
views
SAM file wrong? help with validatesamfile
EXOME
updated 12 months ago by
Ram
43k • written 7.8 years ago by
cristina_sabiers
▴ 110
19
votes
43
replies
5.5k
views
Annotation of huge number of CNV files
CNV annotation TCGA
5.7 years ago by
nazaninhoseinkhan
▴ 520
16
votes
38
replies
17k
views
Differential gene Analysis by Limma
Limma
Microarray
R
updated 24 months ago by
Ram
43k • written 9.2 years ago by
Mo
▴ 920
17
votes
37
replies
4.7k
views
No alias or index file found for protein database
next-gen
software error
sequence
updated 4.9 years ago by
Biostar
20 • written 5.0 years ago by
williamsbrian5064
▴ 510
5
votes
35
replies
3.6k
views
Reduce set of chromosomes in Pangenome graph
pangenome
vg
updated 9 months ago by
Jordan M Eizenga
▴ 460 • written 10 months ago by
anivlete
• 0
3
votes
32
replies
3.4k
views
fastqc report for degradome reads
fastqc
adaptor
6.0 years ago by
Sam
▴ 150
4
votes
30
replies
2.4k
views
SNPs and DEL/MNP in the same position. (DEL or SNP ??)
snp
indels
vcf
calls
mpileup
updated 3.7 years ago by
Ram
43k • written 3.7 years ago by
sami
▴ 40
8
votes
30
replies
5.1k
views
Per Base Sequence Content
sequencing
fastqc
genome
sequence
updated 4.1 years ago by
wm
▴ 560 • written 4.1 years ago by
Researcher
▴ 20
4
votes
30
replies
2.3k
views
Script makes different file then the manual command, but the command is the same
pipeline
linux
awk
3.8 years ago by
stan.aanhane
▴ 30
14
votes
30
replies
3.4k
views
Download GTF/GFF annotation data for NT database, not by organism (for STAR alignment)
RNA-Seq
rna-seq
alignment
next-gen
5.0 years ago by
Malka
▴ 80
0
votes
29
replies
3.0k
views
concordance rate between SNP observed and SNP not observed
SNP
updated 5.9 years ago by
Biostar
20 • written 6.0 years ago by
nour.hadjz
▴ 20
11
votes
29
replies
9.8k
views
SummarizedExperiment nrow differs from ncol
DESeq2
SummarizedExperiment
updated 2.5 years ago by
Ram
43k • written 9.9 years ago by
Parham
★ 1.6k
7
votes
29
replies
2.4k
views
What's the powerful biological methods for significant genes selection?
gene selection
significant
biological method
R
5.4 years ago by
Chaimaa
▴ 260
12
votes
28
replies
2.7k
views
Calculating the expression level of genes
Gene expression
RNA-Seq
R
5.9 years ago by
Za
▴ 140
2
votes
28
replies
7.5k
views
Memory use in indexing
Assembly
software error
updated 5.7 years ago by
Ram
43k • written 5.9 years ago by
marongiu.luigi
▴ 710
3
votes
27
replies
5.6k
views
7 follow
Did anyone manage to successfully run HLAScan and get results?
HLA-Typing
NGS
updated 5.7 years ago by
Shiqi Li
• 0 • written 5.9 years ago by
zeynep
▴ 10
4
votes
27
replies
4.3k
views
MISO test run fails error of bam file was not found
RNA-Seq
Assembly
software error
next-gen
genome
updated 3.4 years ago by
Biostar
20 • written 7.7 years ago by
JoeDoasi
▴ 10
9
votes
27
replies
7.5k
views
How do I use Glimmer 3.02?
sequencing
updated 5.3 years ago by
ojelizodun
• 0 • written 6.1 years ago by
nattzy94
▴ 50
3
votes
26
replies
1.9k
views
6 follow
STAR Genome index Error
STAR
updated 6 months ago by
Ram
43k • written 6 months ago by
Prasanna
• 0
1
vote
26
replies
6.2k
views
plink --assoc command gives "NA" for all the analysis (F_A,F_U,A2,CHISQ,P,OR values)
SNP
plink
association
analysis
5.2 years ago by
kushagraprasad24
• 0
8
votes
25
replies
3.0k
views
construction of a database
sql
noSQL
neo4j
database
updated 13 months ago by
Ram
43k • written 2.9 years ago by
Debut
▴ 20
24
votes
25
replies
8.1k
views
How to generate Beta diversity boxplot from phyloseq object?
phyloseq
beta diversity
metagenomics
3.7 years ago by
dpc
▴ 240
10
votes
25
replies
3.5k
views
The interpretation of PCA
DESeq2
R
PCA
updated 5.8 years ago by
Biostar
20 • written 5.8 years ago by
Za
▴ 140
5
votes
24
replies
8.7k
views
Plink1.9 gives error when converting VCF.gz to PED? "Error read failure"
VCF
Plink
5.9 years ago by
DanielC
▴ 170
13
votes
24
replies
4.6k
views
Error of bam-to-sam conversion using samtools
samtools
TopHat2
RNA-Seq
BAM
SAM
updated 7.1 years ago by
GenoMax
141k • written 7.1 years ago by
Gary
▴ 480
11
votes
24
replies
5.4k
views
BWA mem skip orientation
assembly
genome
alignment
next-gen
5.0 years ago by
williamsbrian5064
▴ 510
0
votes
24
replies
3.5k
views
converting spaces to tabs in gtf files
NCBI
3.0 years ago by
storm1907
▴ 30
1
vote
23
replies
2.0k
views
FASTQ exctract ID's
fastq
4.4 years ago by
User000
▴ 690
6
votes
23
replies
2.9k
views
Could you help me set up a VCF filter?
genome
software error
VCF filter
5.8 years ago by
Charlie2
▴ 50
1
vote
23
replies
1.7k
views
Parse multifasta files based on a values in two columns in a metadata file
parse
mutlifasta
RefSeq
database
strain
3.6 years ago by
jmwhitha
• 0
10
votes
23
replies
2.4k
views
Two simillarly annotated sequence has no alignment similarity. Why?
blast
alignment
gene
updated 6.4 years ago by
GenoMax
141k • written 6.4 years ago by
Farbod
★ 3.4k
11
votes
22
replies
4.2k
views
No significant DEG: A request to double check my commands for limma.
limma
differential-gene-expression
updated 14 days ago by
Ram
43k • written 5.0 years ago by
RNAseqer
▴ 260
23
votes
22
replies
49k
views
6 follow
Converting VCF to PLINK .bed binary fileset to check for pedigree errors with KING: How do conversion tools make the PLINK .fam file, without asking …
plink
vcf
fam
gatk
vcftools
updated 17 months ago by
Nicole
• 0 • written 6.0 years ago by
gaelgarcia
▴ 260
5
votes
22
replies
7.5k
views
SVM for classified gene expression data
R
svm
microarray
updated 21 months ago by
Ram
43k • written 8.3 years ago by
Shaurya Jauhari
▴ 50
1
vote
22
replies
3.5k
views
Genome Index and Alignment- STAR
RNA-Seq
genome
alignment
STAR
updated 5.4 years ago by
Kevin Blighe
87k • written 5.4 years ago by
carolgalah
• 0
2
votes
22
replies
2.3k
views
6 follow
Find ~1 Mb regions of genome that are shared by two or more WGS samples?
pedigree
linkage
genetics
18 months ago by
Joel Wallenius
▴ 210
3
votes
22
replies
3.1k
views
Making RefSeq in Windows
refseq
7.7 years ago by
Alireza Ebadi Tabrizi
• 0
0
votes
22
replies
3.3k
views
Using java Treeview to analyse genes: how to select and compile group of genes to present?
RNA-Seq
6.0 years ago by
Muha0216
• 0
13
votes
22
replies
11k
views
SVA : Setting up the data from an ExpressionSet and applying sva function to extimate batches
sva
combat
batch-effect
updated 6 days ago by
Ram
43k • written 6.8 years ago by
lessismore
★ 1.3k
1
vote
22
replies
6.2k
views
how to use faSplit to split fasta into x files
next-gen
faSplit
sequence
5.5 years ago by
olechnwin
▴ 60
5
votes
22
replies
3.3k
views
Differential analysis between two cell lines
R
RNA-Seq
edgeR
differential analysis
5.8 years ago by
Biologist
▴ 290
4
votes
22
replies
5.5k
views
samtools coverage usage
NGS
updated 2.8 years ago by
Lila M
★ 1.2k • written 2.8 years ago by
smrutimayipanda
▴ 20
2
votes
21
replies
4.1k
views
Compute the correlations between submatrices
correlation
R
matrix
foreach
updated 5.1 years ago by
Ram
43k • written 5.1 years ago by
pablo
▴ 300
3
votes
21
replies
3.8k
views
Normalization agilent microarray data?
Microarray
Normalization
updated 3.0 years ago by
Ram
43k • written 4.6 years ago by
mathavanbioinfo
▴ 90
6
votes
21
replies
2.8k
views
How to evaluate the similarity of genes between two sample
RNA-Seq
differential
updated 5.6 years ago by
Biostar
20 • written 5.7 years ago by
afli
▴ 190
1
vote
21
replies
2.9k
views
Center and scale RIN values for DESeq2?
deseq2
rin
3.7 years ago by
randalljellis
▴ 90
0
votes
21
replies
1.0k
views
Expression analysis of LncRNA from RNA-seq data
expression
lncrna
10 weeks ago by
analyst
▴ 20
12
votes
21
replies
3.2k
views
Calculate disease risk based on the genoytype of some SNPs?
SNP
prs
gwas
PRSice
Plink
updated 4.7 years ago by
Biostar
20 • written 4.8 years ago by
Miguel
▴ 30
0
votes
21
replies
2.7k
views
HTSeq-Count: no_feature too high?
htseq-count
18 months ago by
sea.joson
▴ 10
1,000 results • Page
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Answer: HaplotypeCaller - only SNPs
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Filter the vcf dowstream after haplotypecaller using bcftools or gatk variantfiltration
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We have releases about monthly in order to keep our data accurate and reflect current understanding. In 4 years, there have been about 40 r…
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141k
Let us know when you find out. Would be a useful thing to know what the data looks like.
Comment: How do I use the STARSolo aligner with MGI DNBelab C series HT scRNAseq librarie
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atowns21
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Yes, that is a very good idea. Thanks for the help!
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Only thing would be to try them out. See if you can detect them in the data you have. You could also simply look for unique representativ…
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`clustal omega` can calculate distance matrix between two proteins (makes sense if your proteins are of similar size). You could try that: …
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swbarnes2
14k
If the unpaired are _2, files, the _1 must exist somewhere. But the simple answer is probably going to be to just use all the _2 only. Yo…
Comment: Is it Acceptable to Have Uniform Quality Scores in a FASTQ File?
by
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141k
> The needless granularity (fake precision) was obvious from the start You are assuming that all data generated is always of great quality…
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Istvan Albert
100k
There are various versions of blast, that search in translated spaces, see blastx and tblastn https://blast.ncbi.nlm.nih.gov/Blast.cgi …
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Istvan Albert
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If you have lots of data, both paired and unpaired, then the best strategy is probably to treat them separately and merge the counts at the…
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It is very easy to make nonsensical flags (unfortunately a flaw of the SAM spec itself) ... not to mention the awkward constructs: a primar…
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This forum is dedicated to asking their question on bioinformatics. As long as the topic is bioinformatics, all questions are welcome, and …
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I interpret this as an admission by SRA that phred scores are practically useless. The needless granularity (fake precision) was obvious f…
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by
Ram
43k
If their github repo is alive, raise an issue there.
Comment: TCGA2STAT Error: Firehose connection
by
Ngrin
• 0
Yeah makes sense. I was following their github. Thanks for pointing this out.
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