3 hours ago by
Utpal
•
0
0
votes
4
replies
243
views
0
votes
2
replies
133
views
updated 5 hours ago by
Jordan M Eizenga
▴
70
•
written 15 hours ago by
kcarery
•
0
0
votes
1
reply
161
views
0
votes
1
reply
63
views
0
votes
1
reply
91
views
updated 9 hours ago by
seidel
11k
•
written 11 hours ago by
leenkhoury282
•
0
0
votes
0
replies
96
views
18 hours ago by
Leite
★
1.2k
0
votes
0
replies
9
views
30 minutes ago by
lunarskye222
•
0
0
votes
0
replies
90
views
1
vote
0
replies
77
views
0
votes
0
replies
85
views
0
votes
0
replies
45
views
Recent Votes
Recent Locations •
All
India, 4 minutes ago
United States, 9 minutes ago
Germany, 21 minutes ago
India, 31 minutes ago
United States, 32 minutes ago
Canada, 36 minutes ago
santiago, chile, 37 minutes ago
Recent Awards •
All
Popular Question to
prabin.dm
▴
260
Popular Question to
Info.shi
▴
20
Scholar to
Ming Tommy Tang
★
2.9k
Popular Question to
leeandroid
▴
120
Popular Question to
xingyu
•
0
Popular Question to
bitpir
▴
210
Recent Replies
Comment: Is it possible to do DGE analysis using log 2 normalized data with EdgeR ?
by
Adaline.D
•
0
That explains a lot to me. Thank you, seidel! They used Affymetrix Human Gene 1.0 ST Array chips.
Hope you have a wonderful day.
Comment: How to make cut off linein cluster,
by
Info.shi
▴
20
Thank you so much it worked.
I highly appreciate your help.
Answer: How to make cut off linein cluster,
by
Jeremy
▴
770
Try adding the following line of code:
abline(h = .02)
Comment: Problem with fatsq-dump
by
Utpal
•
0
Thank you for your support.
I tried the command
conda install -c bioconda sra-tools
But, still with the command, fastq-dump
it return…
Comment: RNA Sequencing and Vg pan -transcriptome build
by
Jordan M Eizenga
▴
70
The `vg mpmap` subcommand has features for mapping RNA-seq data to a graph. We describe in more detail in [this publication][1], including …
Comment: how to import hdf5 Hi-C data into R using HiTC package
by
rpolicastro
11k
From the error you may need to specify the file path instead of passing it the imported data.
Comment: RNA Sequencing and Vg pan -transcriptome build
by
cmdcolin
★
2.9k
i am not sure how about the data input format but 'hisat2' is able to 'align rna-seq to a population reference'. this may be a little diffe…
Comment: Is it possible to do DGE analysis using log 2 normalized data with EdgeR ?
by
seidel
11k
What technology is the GEO data set derived from? Signal intensity implies a fluorescence read out (i.e. microarray). If that's the case, y…
Comment: Log2 FC Threshold
by
seidel
11k
Given that 0.2 is a 1.15 fold change (2^0.2) you're right to be skeptical, but there's no clear answer as to the significance. Consider tha…
Comment: Read lengths greater than insert length
by
GenoMax
127k
As I said above the adapters are each ~60 bp long so they add ~120 bp (5' and 3' end adapters) to the length of the DNA fragment. What you …
Comment: Problem with fatsq-dump
by
GenoMax
127k
Looks like the developers have an answer for this error: https://github.com/ncbi/sra-tools/issues/139
At least the program seems to be ins…
Comment: Read lengths greater than insert length
by
shpak.max
▴
30
I'm not claiming that all fragment lengths are exactly 50, the typical range for my data is 45-60. However, my read lengths are all 150, so…
Answer: Read lengths greater than insert length
by
GenoMax
127k
Illumina libraries are made by adding adapters to the ends of DNA fragment(s). Those adapters themselves are about 60 nucleotides long. Not…
Answer: How to get fraction of unspliced reads for specific gene from scRNAseq Cell Rang
by
Rob
5.9k
If you have the raw data, you can easily get this information using [alevin-fry](https://www.nature.com/articles/s41592-022-01408-3). It pr…
Answer: Matching gene-expression data with clinical data: best practices.
by
pilargmarch
▴
60
I really like using [the R/Bioconductor package TCGAbiolinks][1], it gives you clinical and expression data by patient ID.
[1]: http…
Traffic: 900 users visited in the last hour
Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by the
version 2.3.6
version 2.3.6