6 months ago by
langziv
▴
30
0
votes
12
replies
1.1k
views
1
vote
12
replies
1.2k
views
6
votes
12
replies
601
views
2
votes
12
replies
1.8k
views
updated 8 months ago by
Ram
39k
•
written 7.7 years ago by
Kristin Muench
▴
620
1
vote
12
replies
3.2k
views
updated 11 months ago by
Ram
39k
•
written 8.2 years ago by
fashiondesignrussian
▴
60
2
votes
12
replies
645
views
updated 11 months ago by
WouterDeCoster
47k
•
written 11 months ago by
Bioinfo
•
0
2
votes
12
replies
4.2k
views
8
votes
12
replies
2.7k
views
1
vote
12
replies
3.3k
views
1
vote
12
replies
3.7k
views
6
votes
12
replies
1.3k
views
updated 12 weeks ago by
Ram
39k
•
written 2.8 years ago by
akshaykum684
▴
20
7
votes
12
replies
883
views
3
votes
12
replies
2.2k
views
5
votes
12
replies
651
views
0
votes
12
replies
1.6k
views
4
votes
12
replies
1.0k
views
updated 5 months ago by
Ram
39k
•
written 12 months ago by
nils.simon.biology
▴
10
0
votes
12
replies
2.9k
views
updated 20 days ago by
Ram
39k
•
written 5.6 years ago by
fonteneaudam
▴
20
6
votes
12
replies
1.2k
views
updated 11 weeks ago by
Ram
39k
•
written 4.4 years ago by
english.server
▴
290
0
votes
12
replies
2.7k
views
2
votes
12
replies
1.4k
views
0
votes
12
replies
2.3k
views
1
vote
11
replies
3.1k
views
4
votes
11
replies
6.6k
views
9 months ago by
prasundutta87
▴
650
8
votes
11
replies
1.4k
views
6
votes
11
replies
929
views
1
vote
11
replies
3.0k
views
updated 7 months ago by
joe.e.weaver
•
0
•
written 2.9 years ago by
annaA
▴
10
0
votes
11
replies
890
views
5
votes
11
replies
6.4k
views
updated 10 months ago by
Ram
39k
•
written 7.8 years ago by
priyankamaripuri
▴
40
4
votes
11
replies
1.1k
views
4
votes
11
replies
2.6k
views
7 follow
0
votes
11
replies
1.5k
views
10 months ago by
Vaishnavi
•
0
5
votes
11
replies
3.1k
views
0
votes
11
replies
927
views
11 months ago by
pablosolar.r
▴
20
4
votes
11
replies
2.7k
views
updated 4 months ago by
Ram
39k
•
written 5.1 years ago by
mahdikhadem95
▴
30
3
votes
11
replies
895
views
updated 11 months ago by
mark.ziemann
★
1.8k
•
written 11 months ago by
barrypraveen
▴
110
1
vote
10
replies
2.4k
views
3
votes
10
replies
987
views
3 months ago by
Chris
▴
100
4
votes
10
replies
3.7k
views
updated 4 months ago by
Ram
39k
•
written 8.1 years ago by
David_emir
▴
460
7
votes
10
replies
1.2k
views
updated 11 weeks ago by
Ram
39k
•
written 4.2 years ago by
demoraesdiogo2017
▴
90
5
votes
10
replies
4.7k
views
0
votes
10
replies
2.3k
views
updated 11 months ago by
Ram
39k
•
written 8.1 years ago by
bharata1803
▴
550
2
votes
10
replies
3.2k
views
4
votes
10
replies
451
views
5
votes
10
replies
434
views
0
votes
10
replies
2.0k
views
updated 8 months ago by
priya.bmg
▴
60
•
written 2.8 years ago by
sadeghi.reyhane
•
0
5
votes
10
replies
932
views
0
votes
10
replies
3.1k
views
6 follow
2
votes
10
replies
695
views
1
vote
10
replies
2.6k
views
2
votes
10
replies
1.0k
views
11 months ago by
pearl2070
▴
10
Recent Votes
Recent Locations •
All
Canada, just now
United States, 1 minute ago
United States, 1 minute ago
Netherlands, 2 minutes ago
Moldova, 2 minutes ago
Israel, 2 minutes ago
Czechia, 3 minutes ago
Recent Awards •
All
Popular Question to
lait
▴
170
Popular Question to
blur
▴
270
Popular Question to
Tawny
▴
180
Scholar to
rpolicastro
12k
Scholar to
Joel Wallenius
▴
130
Recent Replies
Comment: Which method is the best for using in "dba.count" in Diffbind R package
by
Rory Stark
★
1.7k
If you are calling `dba.normalize()` directly, it must be called *after* calling `dba.count()`. `dba.count()` will apply the default normal…
Comment: switching from illumination to NEB prep kits
by
GenoMax
129k
If you are simply replacing the library prep kit then it will be compatible with normal illumina libraries. If there is a different adapter…
Comment: How to use R installed by conda instead of it installed by apt on Ubuntu
by
Zhichao
•
0
yes, I have activated this conda environment. And the conda is in `PATH` .
At first, I create a new conda environment `conda create -n ne…
Comment: Limma returned only positive logFC values
by
ATpoint
72k
Raw counts, raw meaning not manipulated by any transformation. See limma and edgeR manual, it clearly covers that for using limma-trend and…
Comment: Alternative To interactions comparison in limma in DESeq2
by
ATpoint
72k
https://support.bioconductor.org/p/9143932/ -- see the lower parts where Michael Love answers, it comes down to numeric contrasts.
Comment: Limma returned only positive logFC values
by
melissachua90
▴
40
The `mat` is log2-normalized raw data. Is it feasible? Or should I feed in counts?
Comment: DESeq2 for different design and normalized counts
by
ATpoint
72k
Nope, please read the manual of DESeq2, it clearly tells to use raw counts. What "normalized counts" do you have exactly? limma-trend might…
Comment: How to use R installed by conda instead of it installed by apt on Ubuntu
by
ATpoint
72k
Did you activate your conda environment? Is conda in `PATH`? Please describe what you did.
Comment: Limma returned only positive logFC values
by
ATpoint
72k
Bottom line is, if you are using an intercept-free model (that is what `~0+...` is doing) then you have to use contrasts. In a "normal" mod…
Comment: Asking for feedback on a Python library for computing alignments
by
Alexander
▴
70
Thanks for sharing ! It seems there is a problem to install on Kaggle cloud (like a Colab): I tried several ways: https://www.kaggle.com/co…
Comment: Masking before RNA-Seq Alignment and Gene Prediction in Plant Genomes
by
Darked89
4.4k
I am not sure what you gain by using hard masked genome for RNA-Seq mapping. There will be reads covering some introns. But if you mask rep…
Comment: Extract sequences from a fastq file by a list of IDs
by
mhpakdel96
•
0
Thanks a lot, it works
Comment: Differential protein expression analysis
by
dsull
★
4.2k
I don't have a tutorial but see this paper I co-authored:
https://pubs.acs.org/doi/abs/10.1021/acs.jproteome.0c00666
All of the upstream …
Comment: Differential protein expression analysis
by
Ribo
▴
40
Thank you!
Is there a recommended tutorial for proteomics analysis?
Comment: Limma returned only positive logFC values
by
melissachua90
▴
40
I clustered the samples into subtypes and am now comparing the differential expression across the subtypes.
Traffic: 2498 users visited in the last hour
Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by the
version 2.3.6
version 2.3.6