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541 results • Page
2 of 11
Sort: Votes
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Views
Votes
Replies
2
votes
0
replies
215
views
Reference request for getting the big picture of gene taxonomy
genes
textbook
17 days ago by
garbagelister
▴ 20
2
votes
2
replies
304
views
DESeq factor order
DESeq2
18 days ago by
Chris
▴ 10
2
votes
3
replies
862
views
Freebayes command stopped due to lack of space
freebayes
SNP-calling
updated 11 days ago by
Ram
39k • written 5.0 years ago by
amcheroo
• 0
2
votes
5
replies
443
views
How to check a gene in an open chromatin region or not?
ATAC-seq
27 days ago by
Chris
▴ 100
2
votes
4
replies
234
views
Filter VCF file for variant alleles
VCF
variants
bcftools
vcftools
updated 4 days ago by
Ram
39k • written 4 days ago by
miguellarrazlopezdenovales
• 0
2
votes
4
replies
607
views
DESeq2 batch effects with nonuniform design matrix
DESeqDataSet
DESeq2
batch
updated 23 days ago by
Ram
39k • written 12 months ago by
mcsimenc
▴ 20
2
votes
3
replies
315
views
Low mapping percentage
mapping
STAR
RNAseq
1 day ago by
Sib
▴ 40
2
votes
5
replies
414
views
Cutadapt error: too many parameters.
cutadapt
parallel
bash
6 days ago by
DanielEB_fisk
• 0
2
votes
1
reply
17
views
Is RNA-Seq well suited for studying pseudogenes and lncRNA?
rna
ncRNA
transcriptome
pseudogene
rna-seq
updated 8 minutes ago by
rpolicastro
12k • written 33 minutes ago by
Kermit
▴ 80
2
votes
2
replies
770
views
Final year project query
project
updated 10 days ago by
Ram
39k • written 4.5 years ago by
talhar123456
• 0
2
votes
3
replies
271
views
Using RNA-seq to detect pathogen sequences in host tissue
RNA-seq
11 days ago by
erik.burchard
▴ 10
2
votes
6
replies
380
views
Find data-based Gene_IDs for unknown gene_IDs in gtf.file
RNA-SEQ
annotation
GO-term
14 days ago by
Pegasus
▴ 90
2
votes
4
replies
1.4k
views
Gettin Consensus From Multiple Whole Genomes
multiple-sequence-alignment
whole-genome-alignment
updated 19 days ago by
Ram
39k • written 6.4 years ago by
nilaylale88
• 0
2
votes
1
reply
1.8k
views
How do I apply a patch to hg38?
reference
human
freeze
hg38
patch
updated 3 days ago by
Jeremy Leipzig
21k • written 5.6 years ago by
imperialcommando117
• 0
2
votes
1
reply
267
views
scRNAseq (bioconductor) data to seurat object
bioconductor
seurat
scRNAseq
updated 18 days ago by
ATpoint
72k • written 18 days ago by
Alex
▴ 20
2
votes
8
replies
2.6k
views
How to convert a database from protein to nucleotide
blastn
unix
blastx
blast
updated 25 days ago by
Ram
39k • written 6.2 years ago by
AJTrunkskun94
• 0
2
votes
5
replies
389
views
RNA SEQ reads assembly for illumina sequenced data
NGS
linux
nanopore
updated 25 days ago by
Ram
39k • written 5 weeks ago by
Adyasha
• 0
2
votes
3
replies
690
views
Including bioinformatics into an agriculture/horticulture dissertation project
agrobioinformatics
plant-gene-analysis
updated 5 days ago by
Ram
39k • written 4.1 years ago by
cipri91rusu
• 0
2
votes
3
replies
378
views
Samtools faidx can't be run in succession on two reference files?
Samtools
22 days ago by
DdogBoss
• 0
2
votes
3
replies
110
views
[Waiting on OP] perl script to grep a part of header from fasta seq file
perl
updated 17 days ago by
Ram
39k • written 5.4 years ago by
h_asma
• 0
2
votes
6
replies
1.0k
views
Time series RNA seq design query
RNA-seq
time-series
deseq2
updated 6 days ago by
Ram
39k • written 18 months ago by
kra277
• 0
2
votes
2
replies
765
views
LOG2FCs representation
LOG2FC
DESeq2
PCR
updated 20 days ago by
Ram
39k • written 17 months ago by
Akash D
▴ 40
2
votes
2
replies
392
views
Online tool for creating figures of gene with its features
features
genes
figures
updated 16 days ago by
Ram
39k • written 6 months ago by
andre.arrudalima
▴ 20
2
votes
3
replies
364
views
What's your recommended length and depth for my RNA-seq experiment
RNA-seq
updated 18 days ago by
rfran010
▴ 120 • written 28 days ago by
biotech&Analytics
▴ 10
2
votes
6
replies
569
views
how to make a venn plot with data frame in R
plot
R
Venn
15 days ago by
satva72
• 0
2
votes
2
replies
111
views
how to use Hemtools bed2vcf?
vcf
linux
conversion
bed
updated 6 days ago by
Pierre Lindenbaum
154k • written 6 days ago by
MobiusT
▴ 10
2
votes
7
replies
1.5k
views
Technology Stack of NCBI (Genbank, GEO, etc.)
sequence
ncbi
gene
genbank
updated 13 days ago by
Ram
39k • written 5.2 years ago by
navela78
▴ 70
2
votes
0
replies
724
views
Why are there multiple bax/fastq files associated with my PacBio run?
genome
PacBio
WGS
sequencing
updated 5 days ago by
Ram
39k • written 4.1 years ago by
nickvpinkham
▴ 20
1
vote
0
replies
1.2k
views
sequence alignment using edlib c++ library
cpp
alignment
updated 13 days ago by
Ram
39k • written 4.8 years ago by
BDK_compbio
▴ 140
1
vote
5
replies
526
views
kraken on nt library
kraken
nt
13 days ago by
Francois Piumi
▴ 30
1
vote
0
replies
258
views
How to assess which cell population is most affected by treatmant in single cell rna seq data?
transcriptomics
single-cell
RNA
DEG
9 days ago by
abbas89
▴ 10
1
vote
2
replies
293
views
Identifying/Annotating Enhancers
enhancer
R
ENCODE
bedtools
updated 12 days ago by
ATpoint
72k • written 12 days ago by
cthangav
▴ 40
1
vote
1
reply
990
views
cfDNA target seq vs. cfDNA WES
cfDNA
updated 11 days ago by
Ram
39k • written 4.9 years ago by
genetic
▴ 40
1
vote
3
replies
361
views
how to do an "reverse" oncoplot in maftools
maftools
Rstudio
updated 13 days ago by
Ram
39k • written 16 days ago by
JULIA HELENA
• 0
1
vote
2
replies
177
views
sorting BAM file
BAM
updated 5 days ago by
ATpoint
72k • written 6 days ago by
Mohammad Amin
• 0
1
vote
2
replies
244
views
highlight regions in a dot-plot?
figure
dotplot
alignment
synteny
10 days ago by
Buffo
★ 2.2k
1
vote
3
replies
330
views
Retrieve Promoter Sequences by GeneID
GeneID
by
Sequences
Retrieve
Promoter
updated 13 days ago by
lieven.sterck
14k • written 5 weeks ago by
alessandro.alma00
• 0
1
vote
2
replies
233
views
Building phylogenetic tree accounting for spatial information
BEAST
IQ-TREE
COVID-19
21 days ago by
vembha
• 0
1
vote
3
replies
702
views
Regular Expression for conversion
Linux
grep
updated 5 days ago by
Joe
21k • written 19 months ago by
shabbas12
▴ 10
1
vote
0
replies
1.3k
views
NCBI Taxon ID to IMG Genome IDs
taxon
html
updated 3 days ago by
Ram
39k • written 3.9 years ago by
Mohak
▴ 20
1
vote
0
replies
90
views
Considering gaps in calculating conservation score from MSA
multiple
alignment
sequence
conservation
python
4 days ago by
Jonathan Lefebre
▴ 50
1
vote
1
reply
928
views
microarray data handling
R
microarray
updated 17 days ago by
Ram
39k • written 5.5 years ago by
vincent.bioscience
• 0
1
vote
1
reply
2.6k
views
Comparing time series expression data
time-series
gene-expression
updated 5 days ago by
Ram
39k • written 5.8 years ago by
The
▴ 180
1
vote
4
replies
962
views
Simulate the sequence data
next-gen-sequencing
genetics
updated 13 days ago by
Ram
39k • written 5.1 years ago by
bha
▴ 80
1
vote
3
replies
396
views
repair.sh java.lang.AssertionError
repair.sh
bbtools
updated 23 days ago by
Ram
39k • written 6 months ago by
aimanbarki
▴ 20
1
vote
0
replies
2.5k
views
Running Multiple Alignments in MEGA with a script produces errors.
next-gen
RNA-Seq
MEGA
multiple-alignment
updated 19 days ago by
Ram
39k • written 7.2 years ago by
rachelrodgers12
▴ 10
1
vote
9
replies
536
views
gene annotation
gtf
gene-annotation
updated 23 days ago by
Ram
39k • written 24 days ago by
ahmad
• 0
1
vote
2
replies
2.0k
views
Error in Adding 1000Genomes Ancestral Allele info: Using VCF tools fill-aa
vcftools
samtools
faidx
tabix
1000Genomes
updated 14 days ago by
barslmn
★ 1.6k • written 5.6 years ago by
shrutishreyajha
▴ 10
1
vote
6
replies
570
views
DESeq2
heatmap
R
DESeq2
27 days ago by
Nelo
• 0
1
vote
3
replies
1.5k
views
Using SeqAn and other external libraries in C++
Read-Mapping
SeqAn
updated 18 days ago by
Ram
39k • written 5.6 years ago by
anshupa.vssut
▴ 50
541 results • Page
2 of 11
Recent Votes
Comment: Is RNA-Seq well suited for studying pseudogenes and lncRNA?
Comment: Is RNA-Seq well suited for studying pseudogenes and lncRNA?
Answer: calculate p value and associated z score for snp-gene pair
Answer: calculate p value and associated z score for snp-gene pair
Answer: I need help with a blast command, please help
Answer: I need help with a blast command, please help
Answer: NCBI API, Perl
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Recent Replies
Comment: Difference between USCS exon coordinates and ensembl
by
ATpoint
72k
Yes, BED file, so this is zero-based and on Ensembl Viewer it is 1-based. So that is the reason.
Comment: Obtain a --gene-trans-map file after rnaSPAdes
by
andres.firrincieli
3.3k
Following GenoMax comment This is what you have NODE_217_length_5488_cov_28.472321_g133_i0 # typical rnaSPAdes transcript header Thi…
Comment: calculate p value and associated z score for snp-gene pair
by
rheab1230
▴ 140
Thank you so much for this. Yes, in this case I get all the SNPs. I also have one more question: SNP gene beta t-stat p-value FDR …
Comment: Is RNA-Seq well suited for studying pseudogenes and lncRNA?
by
rpolicastro
12k
lncRNAs are usually fine to include in downstream analysis since they are generally not selected against in most RNA-seq library prep proto…
Comment: I need help with a blast command, please help
by
neto.pvp
• 0
Thank you very much!! I'm new to this subject and I believe your answer gave me a great idea of where to go next, thanks again!!
Answer: NCBI API, Perl
by
GenoMax
129k
You are likely best served by using `NCBI datasets` ([**LINK**][1]) or `EntrezDirect` ([**LINK**][2]) command line tools. If you want to …
Comment: Obtain a --gene-trans-map file after rnaSPAdes
by
GenoMax
129k
You could try to create one: https://groups.google.com/g/trinityrnaseq-users/c/qPbWnLl4iY0 While this is from an unrelated software the so…
Comment: CNNScoreVariants Error
by
Pierre Lindenbaum
154k
that is another question
Comment: Difference between USCS exon coordinates and ensembl
by
amy__
▴ 50
Hi @atpoint, I went to http://genome.ucsc.edu/cgi-bin/hgTables and did: 1. ![enter image description here][1] then: 2. ![enter image…
Comment: should non-protein-coding rna(e.g. lncRNA) be removed in RNA-Seq differential ex
by
Kermit
▴ 80
See also: https://www.biostars.org/p/9535550/#9536086
Answer: Getting species names and taxa id from assembly accession number
by
MirianT_NCBI
▴ 590
Hi, As an alternative, you could use [NCBI Datasets][1] for this task. NCBI Datasets allows users to retrieve metadata reports in JSON or…
Comment: Optimum setting for local blastp for ~10K sequences
by
sodiumnitrate
▴ 20
Thank you for the info! It turned out that running all 10k at once is actually faster than running one or two at a time!
Answer: Installation of PPFinder
by
GenoMax
129k
Since the software is still [**listed on the Brent lab**][1] website your best bet would be to email the investigator and ask that they fix…
Comment: How to calculate TPM from featureCounts output
by
rpolicastro
12k
If you want to go that route, yes.
Comment: Difference between USCS exon coordinates and ensembl
by
ATpoint
72k
What did you download from UCSC? 1-off usually means one file is zero-based and the other 1-based. BED files from UCSC are 0-based, Ensembl…
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