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1,000 results • Page
3 of 20
Sort: Votes
Rank
Views
Votes
Replies
4
votes
4
replies
359
views
Average expression of a sample in single-cell data
single-cell
rna-seq
23 days ago by
newuser2024
• 0
4
votes
12
replies
7.8k
views
6 follow
BWA error: no ID within the read group line
bwa
readgroups
RG
parallel
updated 11 weeks ago by
mgdrnl
▴ 10 • written 3.4 years ago by
whb
▴ 60
4
votes
9
replies
1.2k
views
Snakemake issue with wrappers
Python
Snakemake
5 months ago by
Begonia_pavonina
▴ 150
4
votes
3
replies
303
views
Should you remove PCR duplicates for kmer counting?
kmer
dedup
duplicates
updated 11 weeks ago by
Darked89
4.6k • written 11 weeks ago by
ebaldwin
▴ 30
4
votes
7
replies
2.4k
views
Batch effects vs biological variables
Batch-effect
DESeq2
combat_seq
updated 17 days ago by
Ram
43k • written 3.7 years ago by
l.uva
▴ 20
4
votes
3
replies
686
views
How to interpret output of PLINK association test (--assoc)
assoc
updated 8 months ago by
zx8754
11k • written 8 months ago by
karthivalarmathi99
▴ 10
4
votes
1
reply
623
views
How to interpret Clustree results?
scRNA
clustering
clustree
updated 8 months ago by
LChart
3.9k • written 8 months ago by
leranwangcs
▴ 120
4
votes
3
replies
587
views
Over Representation analysis altered pathways in common between comparisons plot
R
Visualization
DGE
ORA
updated 6 months ago by
Basti
★ 2.0k • written 6 months ago by
Manuel
▴ 10
4
votes
3
replies
745
views
BBduk log and stats appear to be inconsistent
bbduk
bbmap
updated 11 months ago by
GenoMax
141k • written 11 months ago by
Dave Carlson
★ 1.7k
4
votes
4
replies
602
views
Request for a linkage analysis tutorial
linkage
updated 8 months ago by
GenoMax
141k • written 8 months ago by
German.M.Demidov
★ 2.9k
4
votes
13
replies
864
views
RNAseq of primary tumor and metastases in two different organ
metastases
RNA-seq
DEG
R
updated 8 days ago by
BioinfGuru
★ 1.7k • written 14 days ago by
matteo.levorato
• 0
4
votes
8
replies
2.0k
views
How to extract promoter sequences from a plant draft genome?
promoter
genome
updated 10 months ago by
Ram
43k • written 4.6 years ago by
Kumar
▴ 120
4
votes
11
replies
1.8k
views
PCA WITH PLINK
PLINK
updated 8 months ago by
Kevin Blighe
87k • written 8 months ago by
Michelle Guerra
• 0
4
votes
4
replies
479
views
Correlation for ATAC-Seq and Chip-Seq
RNA-Seq
Chip-Seq
ATAC-Seq
8 weeks ago by
qudrat.nii
▴ 10
4
votes
6
replies
955
views
Open-Source software. Good or bad for the market?
genomic-analysis
software
updated 11 months ago by
Ram
43k • written 6.2 years ago by
Bioinformatician90
• 0
4
votes
6
replies
2.1k
views
Single nucleotide Polymorphism (SNP)
SNP
updated 10 months ago by
Ram
43k • written 9.0 years ago by
Harish Sanja
▴ 10
4
votes
6
replies
2.4k
views
Why weblogo of biopython doesn't work?
Biopython
Weblogo
updated 10 months ago by
minakshiboruahassam
• 0 • written 20 months ago by
Plus
▴ 20
4
votes
6
replies
337
views
Protein loops
protein
Loop
6 weeks ago by
Curious
▴ 10
4
votes
7
replies
1.0k
views
Statistical test to compare data across timepoints
Statistics
updated 6 months ago by
Matthias Zepper
4.5k • written 6 months ago by
Sean
▴ 20
4
votes
6
replies
2.6k
views
Convert Nanopore Fast5 files to Fasta format
fast5
fasta
Nanopore
11 months ago by
Sowmya Pulapet
▴ 70
4
votes
6
replies
1.0k
views
Python script to automatically read in, name output files & parse R1 & R2 fastq.gz files when given an input directory with raw data files
python
fastq
9 months ago by
eorr
▴ 30
4
votes
4
replies
845
views
retaining only the clusters of interest
seurat
single-cell
scRna-seq
updated 10 months ago by
Ram
43k • written 10 months ago by
shamza
• 0
4
votes
4
replies
3.3k
views
Unable to install Autogrid4
autogrid
software-error
autogrid4
updated 11 months ago by
Ram
43k • written 3.1 years ago by
roybatty269
• 0
4
votes
11
replies
630
views
Remove X in row name with only number
GSVA
updated 11 weeks ago by
Ram
43k • written 11 weeks ago by
Chris
▴ 260
4
votes
8
replies
3.5k
views
How to reduce the number of SNPs for the Lositan software
Lositan
SNP
updated 10 months ago by
Ram
43k • written 9.5 years ago by
zhaojianli198322
• 0
4
votes
3
replies
483
views
Parsing columns with awk
awk
updated 5 months ago by
Ram
43k • written 5 months ago by
pablo
▴ 300
4
votes
5
replies
1.2k
views
tranfering sam file easy and fast way
SAM
RNA-seq
hisat2
updated 8 months ago by
Ram
43k • written 2.3 years ago by
iamsmor
• 0
4
votes
5
replies
382
views
Filter out miRNA from ncRNA dataset
bowtie
bowtie2
miRNA
filtering
ncRNA
updated 9 weeks ago by
ATpoint
82k • written 9 weeks ago by
binaryCode
▴ 10
4
votes
16
replies
955
views
hisat2 location does not exist
hisat2
updated 8 weeks ago by
Ram
43k • written 9 weeks ago by
Eric
• 0
4
votes
8
replies
1.2k
views
Error executing nf-core/metaboigniter pipeline
nf-core
metaboigniter
nextflow
updated 8 months ago by
Phil Ewels
★ 1.4k • written 8 months ago by
eesha28112001
• 0
4
votes
9
replies
568
views
variant calling
variant-calling
updated 22 days ago by
Ram
43k • written 23 days ago by
dalibenam64
• 0
4
votes
5
replies
2.0k
views
Whole blood single cell RNA-seq dataset?
RNA-Seq
scRNA-Seq
whole blood
single cell
12 months ago by
predeus
★ 1.9k
4
votes
7
replies
544
views
DESeq2 Multifactor Design
RNA-seq
DESeq2
updated 11 days ago by
Ram
43k • written 13 days ago by
AHerik
▴ 20
4
votes
2
replies
555
views
Can I still remove ambient RNA if I do not have raw barcode-gene matrix?
SoupX
6 months ago by
malonzm1
▴ 20
4
votes
7
replies
1.4k
views
.bed files from sequencing platform not containing intervals of "alt", "random" haplotypes. How do I perform coverage and haplotype caller?
exome
.bed
haplotypes
HG38
coverage
updated 9 months ago by
GenoMax
141k • written 9 months ago by
LeandroF.
• 0
4
votes
16
replies
1.7k
views
How to subset large BAM files specifically/ extract specific subsets?
bamtools
RNAseq
samtools
BAM
updated 9 weeks ago by
Ram
43k • written 7 months ago by
ella
• 0
4
votes
2
replies
2.2k
views
Bfast Match Paired End Reads - Reports Half Total Number Of Reads
paired-end
alignment
next-gen
updated 10 months ago by
Ram
43k • written 11.3 years ago by
Kenneth Daily
▴ 50
4
votes
4
replies
602
views
I cannot install anaconda on ubuntu 22.04
ubuntu
anaconda
Conda
updated 8 months ago by
Ram
43k • written 8 months ago by
Omar
▴ 10
4
votes
3
replies
1.0k
views
What is the difference between vst and rlog to perform PCA ?
rlog
PCA
vsd
updated 11 months ago by
ATpoint
82k • written 11 months ago by
Amr
▴ 160
4
votes
6
replies
801
views
how to plot SV(structural variants) from many assemblies of a given locus
SV
11 months ago by
natalev
▴ 20
4
votes
14
replies
2.6k
views
Is it possible to annotate single genes by snpeff
snp
vcf
updated 10 months ago by
Ram
43k • written 6.7 years ago by
misbahabas
▴ 70
4
votes
14
replies
1.1k
views
remove white space in fastq file
fastq
updated 4 months ago by
GenoMax
141k • written 4 months ago by
gkarere
• 0
4
votes
5
replies
533
views
Which refseq_protein db to choose for zingiberaceae
local
blastp
blast
updated 8 months ago by
GenoMax
141k • written 8 months ago by
Nilo
• 0
4
votes
8
replies
1.9k
views
Hello, I have differential ATAC-seq from control and diseased cells (H3K27ac mark as chromatin mark) and wondering how to call enhancers using ROSE …
super-enhancers
Enhancers
updated 12 weeks ago by
jared.andrews07
★ 16k • written 3 months ago by
Manhezz
• 0
4
votes
6
replies
1.2k
views
Transcript quantification
rna-seq
transcriptome
SRA
updated 10 months ago by
ATpoint
82k • written 10 months ago by
firefox91
• 0
4
votes
6
replies
1.8k
views
Failure in installing the ggtree, enrichplot, and ggtree (Bioconductor packages )
Bioconductor
updated 9 months ago by
GenoMax
141k • written 9 months ago by
applepie
• 0
4
votes
7
replies
763
views
Alternative for grep in a for loop
linux
updated 6 months ago by
Ram
43k • written 6 months ago by
Prangan
▴ 20
4
votes
6
replies
932
views
How to pass from DNA to AA fasta
genetics
updated 11 months ago by
Buffo
★ 2.4k • written 11 months ago by
Anderson Stiward
• 0
4
votes
5
replies
2.4k
views
Merge CNVnator output of multiple samples
CNV
WGS
updated 10 months ago by
Ram
43k • written 6.7 years ago by
zhang248
▴ 40
4
votes
7
replies
810
views
SNP IDs
SNP
5 months ago by
kl
▴ 10
1,000 results • Page
3 of 20
Recent Votes
How to calculate reliable Ka/Ks or dN/dS ratio for genes of interest from VCF file
Detecting somatic variants in non-tumor tissue without normals
Answer: Sequence read length shorter than flow cell specification
Answer: Sequence read length shorter than flow cell specification
Comment: Why gatk VariantAnnotator required bam and coverage files
A: Generate Vcf.Gz File And Its Index File Vcf.Gz.Tbi
Visualization of ChIP-seq data using Heatmaps (Updated: 06/10/16)
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Recent Replies
Comment: how to combine multiple RNAseq count files into a single dataframe in R and unix
by
Ram
43k
Thank you, the `csvtk spread` is super useful. I usually import into R using `lapply` then `Reduce` using `merge` but this might be easier.
Comment: Annovar using R package
by
DKA
▴ 40
Thank you for your guidance. The thing is that I am unfamiliar with using such environments, unfortunately.
Comment: Treatment VS Control in Single Cell RNAseq analysis
by
ATpoint
82k
Open a new question, with details.
Comment: Normalize scRNAseq data to housekeeping genes to compare several datasets
by
ATpoint
82k
I recommend https://bioconductor.org/books/3.18/OSCA.basic/normalization.html as well as the "advanced" section in this book.
Comment: Why gatk VariantAnnotator required bam and coverage files
by
QX
• 0
thank you!
Comment: Treatment VS Control in Single Cell RNAseq analysis
by
kilcdincer
▴ 10
Hello, I have more or less same experimental setting and was wondering how you proceeded with your analysis? Can I reach its GitHub reposit…
Comment: BLAST using both nucleotides and taxonomic local databases
by
GenoMax
141k
> I ask this as some BLAST command fields as scinames or sblastnames do not give any output with a classic nt BLAST If that information is…
Answer: BLAST using both nucleotides and taxonomic local databases
by
5heikki
11k
This has been discussed [many times][1] [1]: https://www.biostars.org/p/76551/
Answer: why renaming Idents in Seurat object doesn't work?
by
Bioinfotec
▴ 10
I think when you give indent to seuObj : Idents(seuObj) <- 'RNA_snn_res.0.1' You may wrongly assign other value to it such as …
Answer: ScRNAseq-How to correctly choose cell type marker genes
by
Bioinfotec
▴ 10
In fact, there is no gold standard for cell type annotation. To achieve correct cell type classification, you can combine the two methos. F…
Answer: ScRNA data question
by
Bioinfotec
▴ 10
In violin plot, a dot represents a cell. Actually, the straight lines you see in your infected image just means these cells have the same e…
Comment: Sequence read length shorter than flow cell specification
by
GenoMax
141k
> the have sent data with different sequencing read lengths: 55bp pair ended and 80bp single ended If they sent data that is not what you…
Comment: How to know synonymous and non-synonymous SNPs from transcriptome sequencing
by
rohitsatyam102
▴ 850
I have some off-the-chart dN/dS values for few genes (>20) when using your script on VCF obtained from a 20K plasmodium genomes (pf7K). | …
Answer: Sequence read length shorter than flow cell specification
by
ATpoint
82k
It is the amount of reagent in the kit that determines read length. Sometimes index cycle reagents are allocated to R1/R2 or they used anot…
Comment: Why gatk VariantAnnotator required bam and coverage files
by
Pierre Lindenbaum
161k
you don't need the bam file https://gatk.broadinstitute.org/hc/en-us/articles/13832654601755-VariantAnnotator : VariantAnnotator …
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