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1,000 results • Page
1 of 20
Sort: Votes
Rank
Views
Votes
Replies
23
votes
22
replies
43k
views
6 follow
Converting VCF to PLINK .bed binary fileset to check for pedigree errors with KING: How do conversion tools make the PLINK .fam file, without asking …
plink
vcf
fam
gatk
vcftools
updated 10 months ago by
Nicole
• 0 • written 5.4 years ago by
gaelgarcia
▴ 250
22
votes
20
replies
2.2k
views
what is the prefered strategy to treat with blast-less transcriptomes from RNA-seq
blast
RNA-Seq
genome
gene
updated 5 months ago by
Ram
40k • written 7.2 years ago by
Farbod
★ 3.4k
20
votes
17
replies
2.4k
views
How to add "transcript" feature to a gtf file?
gtf
updated 11 weeks ago by
1769mkc
★ 1.1k • written 2.6 years ago by
pomodoro_sinensis
▴ 110
18
votes
19
replies
1.9k
views
6 follow
Extremely important marker gene has many reads based on IGV viewer but no quantified reads
rna-seq
sequencing
igv
alignment
next-gen
updated 10 months ago by
Ram
40k • written 4.7 years ago by
rbkh09
• 0
18
votes
4
replies
8.3k
views
How To Find Rna-Seq Data Of Published Papers?
geo
next-gen
RNA-seq
updated 7 months ago by
Ram
40k • written 10.1 years ago by
user
▴ 940
14
votes
5
replies
2.9k
views
bioinformatics basic training
genome
updated 6 months ago by
Ram
40k • written 8.8 years ago by
f.muoghalu
• 0
12
votes
13
replies
2.4k
views
Gene Expression and Systems Biology (homework)
gene
homework
updated 9 months ago by
Ram
40k • written 8.2 years ago by
sarathkurichiyil
• 0
11
votes
9
replies
2.3k
views
samtools piping with awk/ bash commands -> Wonky things happen!!!
bash
samtools
awk
updated 9 months ago by
Ram
40k • written 8.3 years ago by
QVINTVS_FABIVS_MAXIMVS
★ 2.5k
10
votes
14
replies
4.3k
views
ATAC-seq data and deepTools: Small detail with impact on output
bigwig
read-length
deeptools
ATAC-seq
updated 3 months ago by
Ram
40k • written 3.6 years ago by
gable_works
▴ 50
10
votes
6
replies
691
views
RNA seq, secreted protein
protein
gene
secreted
updated 7 weeks ago by
Joe
21k • written 9 months ago by
Rob
▴ 160
10
votes
15
replies
5.6k
views
convert fasta/gb to vcf
fasta
vcf
genbank
gff
updated 6 months ago by
Ram
40k • written 5.0 years ago by
marongiu.luigi
▴ 690
10
votes
6
replies
2.7k
views
which language to use for such a bioinformatics web services infrastructure?
web-services
java
updated 6 months ago by
Ram
40k • written 8.3 years ago by
lait
▴ 180
10
votes
15
replies
1.3k
views
Can't find a variant which suppose must have in a vcf file
variant-calling
bcftools
nf-core
3 months ago by
Chris
▴ 180
10
votes
7
replies
3.4k
views
What is Pathway Analysis?
database
analysis
ontology
pathway
updated 3 months ago by
Ram
40k • written 7.5 years ago by
Pranavathiyani G
▴ 330
9
votes
7
replies
998
views
Reference genome location
reference
positions
FASTA
alignment
genome
updated 8 months ago by
barslmn
★ 1.8k • written 9 months ago by
Batel
• 0
9
votes
13
replies
13k
views
Sort bam file by coordinates using samtools
next-gen-sequencing
gene
rna-seq
updated 3 months ago by
Ram
40k • written 5.0 years ago by
Shahzad
▴ 30
9
votes
5
replies
421
views
Prophage prediction tool
prophage
PHASTER
prediction
updated 9 weeks ago by
GenoMax
134k • written 9 weeks ago by
A_heath
▴ 140
9
votes
4
replies
1.6k
views
On which branch in bioinformatics should a cancer researcher focus on ?
cancer
updated 5 months ago by
Ram
40k • written 7.1 years ago by
Bilal
▴ 60
9
votes
4
replies
5.3k
views
How to analyse normalized read count?
RNA-Seq
R
updated 8 months ago by
Ram
40k • written 8.3 years ago by
pbio
▴ 150
9
votes
16
replies
3.3k
views
6 follow
covert SAM to full length fasta
fasta
SAM
updated 6 months ago by
Ram
40k • written 5.1 years ago by
marongiu.luigi
▴ 690
9
votes
20
replies
3.6k
views
Solve SAM issues flagged by Picard's ValidateSamFile
Picard
SAM
updated 6 months ago by
Ram
40k • written 5.1 years ago by
marongiu.luigi
▴ 690
8
votes
10
replies
1.5k
views
PyWGCNA
WGCNA
3 months ago by
yoshifumimiya
▴ 40
8
votes
4
replies
1.8k
views
using different tools to identify differential exprssed genes
rna-seq
RNA-Seq
R
updated 12 months ago by
Ram
40k • written 8.1 years ago by
Anushka
▴ 20
8
votes
7
replies
739
views
Why does Ensembl VEP provide HGVSg for some variants and not others?
vcf
VEP
3 months ago by
Jeremy Leipzig
21k
8
votes
25
replies
2.4k
views
construction of a database
sql
noSQL
neo4j
database
updated 6 months ago by
Ram
40k • written 2.4 years ago by
Debut
▴ 20
8
votes
13
replies
1.2k
views
Mapped reads not mapping to a real sequence?
bam
sam
samtools
kallisto
7 months ago by
txema.heredia
▴ 80
8
votes
5
replies
11k
views
samtools tview symbols
samtools
updated 10 months ago by
Ram
40k • written 8.2 years ago by
biolab
★ 1.4k
8
votes
3
replies
844
views
Package installation error using Bioconda
antismash
Bioconda
9 months ago by
A_heath
▴ 140
8
votes
5
replies
3.3k
views
Is it possible to guess sequencing platform used based on a FASTQ/BAM file?
BAM
FASTQ
updated 9 months ago by
Ram
40k • written 8.3 years ago by
Andrew
▴ 60
8
votes
12
replies
3.1k
views
Use BLAST Command Line Applications to run a folder of many sequences against a database
BLAST
updated 6 months ago by
Ram
40k • written 2.1 years ago by
daver.v
▴ 30
8
votes
9
replies
2.8k
views
error in rstudio
csv
matrix
rstudio
updated 12 months ago by
Ram
40k • written 8.1 years ago by
fi1d18
★ 4.2k
8
votes
5
replies
1.6k
views
BLASTp command line : filtering results by a minimum % of identity and % coverage
BLASTp
updated 5 days ago by
Ram
40k • written 24 months ago by
A_heath
▴ 140
8
votes
6
replies
1.0k
views
How to apply bioinformatic in vaccine design?
Vaccine
updated 10 months ago by
Dunois
★ 2.4k • written 10 months ago by
Jean
▴ 50
7
votes
7
replies
4.6k
views
RNA-Seq time series analysis using a DESeq2 spline approach yields far too many significant genes
splines
DESeq2
time-series
RNA-Seq
updated 3 months ago by
Ram
40k • written 4.7 years ago by
stu111538
▴ 80
7
votes
10
replies
1.4k
views
col as names [solved]
R
updated 6 months ago by
Ram
40k • written 4.5 years ago by
demoraesdiogo2017
▴ 90
7
votes
4
replies
1.4k
views
So many Zeros (read counting)_NGS sequence analysis with R / Bioconductor: RNA-Seq workflow
alignment
RNA-Seq
R
updated 6 months ago by
Ram
40k • written 5.2 years ago by
healing80
• 0
7
votes
2
replies
3.2k
views
Counting reads for gene transcripts with overlapping loci
RNA-Seq
gene
R
updated 8 months ago by
Ram
40k • written 8.3 years ago by
Ruet
▴ 70
7
votes
8
replies
8.6k
views
Determining read count
trimmomatic
rna-seq
trinity
updated 4 weeks ago by
Ram
40k • written 7.4 years ago by
nikelle.petrillo
▴ 110
7
votes
12
replies
1.1k
views
Integrated genes from bulk RNA seq and ATAC seq
bulk-RNA
integrated
ATAC-seq
updated 4 months ago by
Sasha
▴ 720 • written 4 months ago by
Chris
▴ 180
7
votes
6
replies
1.7k
views
How to get/calculate coverage of a gene using bed file?
bed
coverage
gene
updated 6 months ago by
Ram
40k • written 22 months ago by
Nikhil
▴ 10
7
votes
8
replies
1.8k
views
How can we time a recent gene duplication event using (neutral?) DNA mutations as molecular clock?
molecular-clock
gene-duplication
phylogeny
updated 7 months ago by
Ram
40k • written 8.4 years ago by
xiaofeng.dong12
▴ 20
7
votes
4
replies
1.4k
views
microarray gene datasets
gene
updated 7 months ago by
Ram
40k • written 8.4 years ago by
bioinfo
▴ 60
7
votes
3
replies
1.4k
views
What are the best tools for Genome assembly
Assembly
genome
updated 3 months ago by
Ram
40k • written 5.1 years ago by
Shawrob400
▴ 10
7
votes
10
replies
1.5k
views
Ubuntu 20.04 Crash
Ubuntu
updated 7 months ago by
Ram
40k • written 20 months ago by
bala
• 0
7
votes
6
replies
850
views
On "wings" in volcano plots...
DEG
volcano
DGE
statistics
DE
updated 4 months ago by
ATpoint
76k • written 4 months ago by
kalavattam
▴ 180
7
votes
5
replies
2.3k
views
using machine learning regards to ribo-seq data sets
gene-regulatory-network
ribo-seq
machine-learning
updated 11 months ago by
Ram
40k • written 8.1 years ago by
fi1d18
★ 4.2k
7
votes
8
replies
3.6k
views
DEseq2 design matrix with 3 factors
RNA-seq
deseq2
updated 3 months ago by
Ram
40k • written 3.3 years ago by
kand3e
▴ 60
7
votes
7
replies
1.7k
views
Sort and convert the SAM files to BAM
samtools
RNA-seq
NGS
updated 4 weeks ago by
Ram
40k • written 5.2 years ago by
amitunited0532
▴ 40
7
votes
4
replies
1.8k
views
useful and freely available softwares for Bioinformatics
software
updated 6 months ago by
Ram
40k • written 8.5 years ago by
Mo
▴ 920
7
votes
8
replies
3.5k
views
remove duplicated entries from BLAST result file
blast
alignment
updated 9 months ago by
Ram
40k • written 8.3 years ago by
Kumar
▴ 170
1,000 results • Page
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Recent Votes
C: sra-explorer : find SRA and FastQ download URLs in a couple of clicks
C: sra-explorer : find SRA and FastQ download URLs in a couple of clicks
Answer: Which function is best for pathway analysis?
Comment: Hisat2 index and alignment question
Answer: Low Alignment rate
Answer: Unable to build applet in DNAnexus, .jar file not found
Answer: Unable to build applet in DNAnexus, .jar file not found
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Recent Replies
Comment: Dataset's name in BioMart for S. pombe
by
YUYANG.OON
• 0
Works with `useMart(biomart="fungi_mart", dataset="spombe_eg_gene", host="https://fungi.ensembl.org")`
Comment: Bug of vg surject
by
Jordan M Eizenga
▴ 360
This is still hard to interpret without the commands. What would help is if you literally copied the full UNIX commands along with their re…
Comment: Which function is best for pathway analysis?
by
Chris
▴ 180
Thank you for your reply! Do you have code that I and others can implement? I am not sure how to customize the gmt file from my differentia…
Comment: Bug of vg surject
by
Qi
• 0
Thanks your reply! my resulte show below: 5x_pan_sv.bam 1347587601 #converted from gam to bam F1 indel=0.830392 snp=0.932511 5…
Comment: Bug of vg surject
by
Jordan M Eizenga
▴ 360
It might help us to diagnose the problem if you included the exact commands that you used and showed the results that you are concerned abo…
Answer: Which function is best for pathway analysis?
by
Yogi
▴ 20
Few things to mention. Pathway Enrichment Analysis can be split into two categories. 1. Functional Class Scoring Methods 2. Overrepr…
Answer: identify DEGs across all conditions and per specific conditions
by
Yogi
▴ 20
Couple of things. (Feel free to correct if I'm misunderstanding). "DESeq2" is meant for 1v1 comparisons. If you're trying to compar…
Comment: Hisat2 index and alignment question
by
Istvan Albert
98k
yes correct you have to pass the full prefix and not the directory
Comment: Low Alignment rate
by
dsull
★ 4.7k
Wow! Didn't realize the repeat-masked file could have such a large influence on alignment. Very good to know -- I guess I'll need to dive i…
Comment: Troubles launch IGV on Linux(Debian)
by
morovatunc
▴ 550
Disclaimer: My comment could be totally unrelated. I would give these a try if you have noone comment/answer your problem. I have had this…
Comment: How to check RNAseq support for annotated genes?
by
Michael
53k
> I tried it using STAR to map the transcript to the annotation gtf file That is not what STAR is for. STAR is for aligning short reads in…
Comment: Low Alignment rate
by
Mehwish
• 0
Thanks alot! This worked out for me https://ftp.ensembl.org/pub/release-110/fasta/homo_sapiens/dna/Homo_sapiens.GRCh38.dna.toplevel.fa.gz …
Comment: Violin plot (Monocle 3) - Troubleshooting
by
Ram
40k
The error message tells me there is the possibility of a conflicting function called `plot_genes_violin` that accepts one parameter and err…
Comment: Violin plot (Monocle 3) - Troubleshooting
by
bk11
★ 1.2k
I m not sure why you have problem there. I do not encounter any problem. May be your gene list are not present in your source object. Pleas…
Comment: Violin plot (Monocle 3) - Troubleshooting
by
Ram
40k
What is the output to: ```r help.search('plot_genes_violin', agrep=FALSE, ignore.case=FALSE, fields=c('name')) lsf.str() sessionInfo() ```
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