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308 results • Page
3 of 7
Sort: Votes
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Views
Votes
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0
votes
1
reply
217
views
Significance testing of top vs. random SNPs
SNPs
significance
variance
statistical
updated 5 days ago by
dthorbur
▴ 550 • written 9 days ago by
am29
▴ 30
0
votes
2
replies
398
views
WES CNV analysis
CNV
WES
20 days ago by
Avinash
• 0
0
votes
0
replies
186
views
TCGAbiolinks not working anymore
R
TCGAbiolinks
GDC
updated 18 days ago by
Ram
40k • written 18 days ago by
master chief
• 0
0
votes
1
reply
171
views
vg rna pantranscriptome
rna
vg
updated 7 days ago by
Jordan M Eizenga
▴ 360 • written 8 days ago by
z
• 0
0
votes
1
reply
208
views
High amount of intronic/intergenic reads in SMARTer stranded total bulk RNAseq
RNA-seq
DNA
SMARTer
updated 4 days ago by
Ram
40k • written 4 days ago by
Mat
▴ 60
0
votes
0
replies
273
views
GTF/Reference genome adjustments? Gene-based differential expression analysis of genetically modified mouse line
RNA-seq
DGE
updated 8 days ago by
Ram
40k • written 9 days ago by
alex
• 0
0
votes
0
replies
167
views
Polishing unicycler hybrid assembly results
hybrid
unicycler
polish
24 days ago by
YAYA
• 0
0
votes
0
replies
551
views
after gatk VariantAnnotator -V *_com_norm.vcf -A AlleleFraction -O *_norm_AB.vcf There "nan,nan" or "nan" in my vcf file
gatk
AlleleFraction
VariantAnnotator
updated 7 days ago by
Ram
40k • written 8 days ago by
zhuo
• 0
0
votes
1
reply
224
views
Biopython and Fab detection
biopython
fab-detection
antibody
updated 26 days ago by
Jesse
▴ 720 • written 26 days ago by
PamCraven
• 0
0
votes
1
reply
202
views
Annotation of L1 from short read (single-strand) RNA
short-read
Line1
fastq
annotation
updated 29 days ago by
rfran010
▴ 810 • written 29 days ago by
Kilian
• 0
0
votes
3
replies
325
views
Pearson correlation with different row numbers across two datasets?
perasoncorrelation
pearson
error
updated 14 days ago by
Kevin Blighe
86k • written 14 days ago by
siefeldin
▴ 30
0
votes
1
reply
187
views
Geo2r rna seq analysis
Geo2r
RNA-seq
updated 15 days ago by
Ram
40k • written 16 days ago by
angiannak
• 0
0
votes
2
replies
241
views
Segmentation fault error in CONTROL-FREEC
copy-number-variants
FREEC
15 days ago by
DdogBoss
• 0
0
votes
1
reply
192
views
Merging the filename with tsv files for master file
genomics
updated 1 day ago by
barslmn
★ 1.8k • written 3 days ago by
smrutimayipanda
▴ 20
0
votes
3
replies
323
views
Not all variants are annotated with AF - expected or a problem?
Alignment
WGS
Calling
Variant
updated 18 days ago by
GenoMax
134k • written 19 days ago by
Luiz
▴ 30
0
votes
1
reply
301
views
how to determine n_cells_by_count
scanpy
updated 12 days ago by
yl759
▴ 40 • written 11 weeks ago by
dalibenam64
• 0
0
votes
0
replies
107
views
How to annotate BED for tissue expression?
BED
annotation
1 day ago by
Fabio_bie88
• 0
0
votes
2
replies
338
views
What is happening in the Zuker-Algorithm
R
folding
RNA
updated 25 days ago by
4galaxy77
2.8k • written 25 days ago by
Serij´s
• 0
0
votes
0
replies
122
views
How to compare degradation rate or half-life
degradation-rate
half-life
updated 20 days ago by
Ram
40k • written 20 days ago by
g744695539
• 0
0
votes
0
replies
255
views
getSex warning in minfi - what is the distinction between the two sex prediction methods?
methylation
ewas
minfi
25 days ago by
rkb965
• 0
0
votes
5
replies
575
views
Bowtie2 with secondary alignment option produces multiple mapping with offset and varying alignment scores
bowtie2
score
alignment
multiple-alignment
23 days ago by
polag01
▴ 10
0
votes
1
reply
904
views
Issue interpreting plasmidSPAdes output
plasmidSPAdes
updated 5 days ago by
Ram
40k • written 2.9 years ago by
A_heath
▴ 140
0
votes
0
replies
123
views
how simply mutation annotation in R?
annotation
RNA-seq
Bioconductor
13 days ago by
octpus616
▴ 90
0
votes
2
replies
265
views
Poly-A trimming for WGBS
Trimgalore
FastP
Poly-A
27 days ago by
Keem Uarren Eiryll
• 0
0
votes
3
replies
352
views
How to determine number of SNPs called?
Snippy
SNP
27 days ago by
SushiRoll
▴ 110
0
votes
4
replies
464
views
Weirdness in annotation (missing allele frequencies)
allele-frequency
gnomad
annovar
updated 3 days ago by
Jeremy Leipzig
21k • written 4 days ago by
Can Abdullah
• 0
0
votes
1
reply
175
views
PyMOL: how to list all selection names?
pymol
updated 8 days ago by
Wayne
★ 1.8k • written 9 days ago by
Ondina
▴ 90
0
votes
0
replies
124
views
RnBeads Differential Methylation
rnbeads
RNA-seq
methylation
updated 21 days ago by
Ram
40k • written 22 days ago by
aroso491
• 0
0
votes
1
reply
180
views
PAML/codeml Poxvirus dN/dS
dNdS
codeml
selection
updated 20 days ago by
manaswwm
▴ 440 • written 20 days ago by
Adrian Pelin
★ 2.6k
0
votes
1
reply
267
views
WGCNA
WGCNA
Degree
igraph
updated 24 days ago by
LauferVA
3.7k • written 24 days ago by
Mehwish
▴ 10
0
votes
2
replies
292
views
Deseq2 colData for single condition
RNA-seq
11 days ago by
Petesview
• 0
0
votes
0
replies
203
views
Tools to turn perfect phylogenetic matrix into a tree
parsimony
Phylogeny
tree
11 days ago by
Ritu_K
▴ 10
0
votes
0
replies
155
views
Help with single cell annotation
single-cell
updated 19 days ago by
Ram
40k • written 20 days ago by
Chris
▴ 180
0
votes
0
replies
202
views
External validation in bioinformatics analyses
External
validation
12 days ago by
Saeedeh Salehi
▴ 10
0
votes
0
replies
141
views
Help with Mofa2
multiome
mofa2
updated 11 days ago by
Ram
40k • written 15 days ago by
Chris
▴ 180
0
votes
1
reply
187
views
quantifying splicing transcripts for nanopore RNAseq
RNAseq
splicing
nanopore
updated 13 days ago by
GenoMax
134k • written 14 days ago by
jshen
• 0
0
votes
1
reply
170
views
Pairwise Sequence Alignments with Numbered Antibody Sequences
sequence
alignment
antibody
updated 18 days ago by
Jesse
▴ 720 • written 22 days ago by
jsweet6
• 0
0
votes
1
reply
191
views
FATAL ERROR on phASER
phASER
updated 27 days ago by
Pierre Lindenbaum
157k • written 27 days ago by
Shani
• 0
0
votes
3
replies
272
views
Genomic location of MUC16
VNTR
genome
updated 19 days ago by
GenoMax
134k • written 22 days ago by
fi1d18
★ 4.2k
0
votes
2
replies
276
views
Gene Ontology - How to do Transcript Ontology?
gene-ontology
GO
transcripts
updated 15 days ago by
Ram
40k • written 17 days ago by
Lakritz the LabRat
• 0
0
votes
0
replies
191
views
What programs allow you to find the mutation rate of many sequences without needing to calculate by hand?
mutation-rate
SARS-CoV-2
updated 26 days ago by
Ram
40k • written 26 days ago by
Madeline
• 0
0
votes
0
replies
120
views
affy expression array data
oligo
limma
RNA
22 days ago by
liuyibin2005
• 0
0
votes
0
replies
150
views
Find reference genome regions spanned by only mapping quality 0 reads in multiple WGS samples
mapping-quality
BAM
FASTA
23 days ago by
William
★ 5.2k
0
votes
0
replies
134
views
rDNA copy number variation (CNV) based on hg38
hg38
CNVkit
rDNA
CNV
genomics
13 days ago by
rbioinfo
▴ 10
0
votes
7
replies
786
views
Mugsy error -directory must be a directory
Mugsy
updated 5 days ago by
Ram
40k • written 2.8 years ago by
A_heath
▴ 140
0
votes
0
replies
124
views
Data for pathview() (KEGG Pathway Map) after EdgeR
EdgeR
LogFC
pathview
KEGG
8 days ago by
Ann
▴ 10
0
votes
0
replies
129
views
Implementing covariates in calculating differential methylation
rrbs
methylkit
epigenomics
22 days ago by
Srinka
▴ 20
0
votes
3
replies
117
views
Bug of vg surject
vg
updated 7 hours ago by
Jordan M Eizenga
▴ 360 • written 9 hours ago by
Qi
• 0
0
votes
1
reply
211
views
Long read filtering based on reference
Long-read
ONT
updated 18 days ago by
Ram
40k • written 19 days ago by
Link
• 0
0
votes
0
replies
118
views
Any way to predict hormone biosynthesis pathways in understudied organisms?
genomics
orthologs
biosynthesis-pathway
updated 6 days ago by
Ram
40k • written 7 days ago by
Mark
• 0
308 results • Page
3 of 7
Recent Votes
Peak annotation with HOMER. Statistics with the annStats parameter
Answer: Fetch Fastq files directly for SRA data
Answer: Fetch Fastq files directly for SRA data
A: Using TargetScan on other species
Comment: Low Alignment rate
Comment: ncbi error report log for validate fastq issue
GEO data mining (IB) - Downloading Sequence Read Archive (SRA) raw data
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Recent Replies
Comment: Sequence quality drops in the 3' end of the left sequence in paired end. What co
by
Asaf
10k
What machine? (Nextseq? Novaseq?) what library prep method?
Comment: How to determine the total count for each gene in lymphotype B
by
fracarb8
★ 1.2k
If you want the average expression, you should look at `AverageExpression` from seurat which returns the average instead of the sum. `Avera…
Answer: Fetch Fastq files directly for SRA data
by
Umer
▴ 50
You can also use **[Grabseqs][1]**. Easiest way is to use it with anaconda as **conda install -c louiejtaylor grabseqs** But you have…
Answer: chromosome location
by
Umer
▴ 50
What you can do is use **SnapGene** 1. Download the genome (fasta file) 2. Load it into Snapgene 3. Find gene locations based on the …
Comment: Troubles launch IGV on Linux(Debian)
by
barslmn
★ 1.8k
Your log seems fine. Could you add the results of these commands? - Which version of debian are you on? ``` lsb_release -c `…
Comment: How to plot coverage and depth statistics of a bam file
by
jl19
• 0
Hi William, I was trying to use bam2plot you have developed. It was installed on a python evnviroment python version 3.11. The installat…
Comment: Help writing code for a question on my homework
by
Joe
21k
This isn't really the right place for this question. Please ask this as a separate new question, but only if it is bioinformatics related.
Comment: How to determine the total count for each gene in lymphotype B
by
dalibenam64
• 0
thank you very much for your response I'm really grateful !! but I'm still confused because for example for patients I have 2 replicates…
Comment: Sequence quality drops in the 3' end of the left sequence in paired end. What co
by
eggrandio
▴ 40
But shouldn't quality drop at both 3'ends and not just on the left one?
Comment: Sequence quality drops in the 3' end of the left sequence in paired end. What co
by
ATpoint
77k
https://www.biostars.org/p/91624/
Comment: to annotate BEDPE files
by
Lhl
▴ 760
is this what you are looking for? https://github.com/imgag/ngs-bits/blob/master/doc/tools/BedpeGeneAnnotation.md
Comment: ncbi error report log for validate fastq issue
by
1769mkc
★ 1.1k
" you're forced to use it" this is sort of since I have use docker image and then pass the GSM id as list of input first to check if the th…
Comment: ncbi error report log for validate fastq issue
by
ATpoint
77k
I don't think this log is helpful. Can't you just get fastq download links via sra-explorer.info or the tool mentioned by @rob in his answe…
Comment: GO ENRICHMENT ANALYSIS- DESEQ
by
Basti
★ 1.7k
You need to show an example of your list
Comment: How the first sequencing template removed in pair end sequencing
by
rfran010
▴ 810
Great! It looks like USER enzyme is used to cleave before the first read sequencing and formamidopyrimidine DNA N-glycosylase is used to cl…
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