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89 results • Page
2 of 2
Sort: Votes
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Votes
Replies
0
votes
0
replies
121
views
maftools - median survival time doesn't match plot
R
survival
maftools
K-M
4 days ago by
BioGuy
• 0
0
votes
0
replies
138
views
PRSice - Phenotype File Not Read Correctly
PRSice2
6 days ago by
V
• 0
0
votes
1
reply
898
views
Issue interpreting plasmidSPAdes output
plasmidSPAdes
updated 2 days ago by
Ram
40k • written 2.9 years ago by
A_heath
▴ 140
0
votes
1
reply
168
views
Identify parent of each read in a GAF
gaf
vgteam
vg
updated 3 days ago by
Jordan M Eizenga
▴ 360 • written 4 days ago by
cfourps
▴ 10
0
votes
3
replies
225
views
Errors while trying to run Scenic
Scenic
updated 3 days ago by
GenoMax
134k • written 4 days ago by
Stavroula
• 0
0
votes
3
replies
179
views
Weirdness in annotation (missing allele frequencies)
allele-frequency
gnomad
annovar
updated 23 hours ago by
Ram
40k • written 1 day ago by
Can Abdullah
• 0
0
votes
7
replies
779
views
Mugsy error -directory must be a directory
Mugsy
updated 2 days ago by
Ram
40k • written 2.8 years ago by
A_heath
▴ 140
0
votes
0
replies
109
views
Current landscape of approaches to scRNA-seq with nanopore sequencers?
scRNA-seq
nanopore
single-cell
updated 1 day ago by
Ram
40k • written 1 day ago by
LauferVA
3.7k
0
votes
1
reply
140
views
How to download genomes and proteins from JGI in bulk via the command line?
jgi
cli
updated 1 day ago by
Ram
40k • written 1 day ago by
O.rka
▴ 680
0
votes
0
replies
130
views
Illumina methylation EPIC V2 array
ewas
methylation
enrichment
missMethyl
EPIC
2 days ago by
juliviglino
• 0
0
votes
1
reply
68
views
Hugo_Symbol to Entrez ID
hugo
bioinformatics
entrez
ids
symbols
updated 1 hour ago by
GenoMax
134k • written 12 hours ago by
shakyaram079
• 0
0
votes
0
replies
104
views
Any way to predict hormone biosynthesis pathways in understudied organisms?
genomics
orthologs
biosynthesis-pathway
updated 3 days ago by
Ram
40k • written 3 days ago by
Mark
• 0
0
votes
1
reply
177
views
Esearch, Epost, and Efetch for Large Datasets in Biopython
eutils
biopython
entrez
updated 2 days ago by
Ram
40k • written 2 days ago by
Salem
• 0
0
votes
1
reply
127
views
Modify the code to take most abundant reads from a cluster and process it.
cd-hit-est
clustering
updated 4 hours ago by
Mensur Dlakic
★ 24k • written 17 hours ago by
Mohd
▴ 40
0
votes
1
reply
754
views
How to identify specific region using Mauve?
Mauve
primers
updated 2 days ago by
Ram
40k • written 2.8 years ago by
A_heath
▴ 140
0
votes
1
reply
376
views
Close genome comparison
Mauve
Mugsy
genome-comparison
GSAlign
updated 2 days ago by
Ram
40k • written 19 months ago by
A_heath
▴ 140
0
votes
5
replies
441
views
Convert gene id's to gene symbol preserving gene id's in deseq2
ensembl
r
DE
deseq2
updated 6 days ago by
Ram
40k • written 8 days ago by
dylannicoembros
• 0
0
votes
0
replies
263
views
GTF/Reference genome adjustments? Gene-based differential expression analysis of genetically modified mouse line
RNA-seq
DGE
updated 5 days ago by
Ram
40k • written 5 days ago by
alex
• 0
0
votes
0
replies
97
views
Gene enrichment analysis of prokaryotes gene
prokaryotes
GO
enrichment
DEG
1 day ago by
Genta
• 0
0
votes
0
replies
77
views
Compare peaks between clusters in sc-ATAC
Peak-Calling
Epigenome
scATAC-seq
updated 19 hours ago by
Ram
40k • written 20 hours ago by
Ahmed.waraky
▴ 10
0
votes
1
reply
132
views
High amount of intronic/intergenic reads in SMARTer stranded total bulk RNAseq
RNA-seq
DNA
SMARTer
updated 1 day ago by
Ram
40k • written 1 day ago by
Mat
▴ 60
0
votes
0
replies
421
views
after gatk VariantAnnotator -V *_com_norm.vcf -A AlleleFraction -O *_norm_AB.vcf There "nan,nan" or "nan" in my vcf file
gatk
AlleleFraction
VariantAnnotator
updated 4 days ago by
Ram
40k • written 4 days ago by
zhuo
• 0
0
votes
3
replies
724
views
How to add translation of CDS in Artemis?
translation
Artemis
updated 2 days ago by
Ram
40k • written 2.7 years ago by
A_heath
▴ 140
0
votes
2
replies
180
views
Identify genes for mapped reads with combined human-7HPV genome index
star
samtools
updated 5 days ago by
Ram
40k • written 5 days ago by
r.j.lock
• 0
0
votes
3
replies
296
views
RdRp scan - identifying/detecting viruses- metagenomic workflow- need help
RdRp
virus-detection
metagenomic
updated 5 days ago by
Ram
40k • written 9 days ago by
eric--carron
• 0
0
votes
2
replies
217
views
Is it okay to just average the log2FC values across different cell types in pseudobulk scRNA-seq data?
scrna-seq
rna-seq
log2fc
3 days ago by
JLee
• 0
0
votes
3
replies
2.0k
views
extraction of original gene iDs from reference annotation file
rna-seq
updated 3 days ago by
aishwarya
• 0 • written 5.2 years ago by
blooming.daisy333
▴ 110
0
votes
0
replies
356
views
Distinction of two very close bacterial strains
mutations
bacteria
updated 2 days ago by
Ram
40k • written 2.3 years ago by
A_heath
▴ 140
0
votes
6
replies
279
views
genome data downloads for various strains
gisaid
hass-marr
updated 1 day ago by
Ram
40k • written 2 days ago by
iftikharmaryam123
• 0
0
votes
0
replies
62
views
Convert RNASeq V2 data in cBioPortal having RSEM normalized results to TPM values
bioinformatics
TPM
RNASeqV2
cbioportal
RSEM
updated 1 hour ago by
4galaxy77
2.8k • written 10 hours ago by
shakyaram079
• 0
0
votes
0
replies
136
views
Selecting SNPs for two-sample Mendelian randomization study/analysis
Two-sample
SNPs
Mendelian
randomization
5 days ago by
huynguyenacademia
• 0
0
votes
6
replies
842
views
FACS (Fluorescence-activated cell sorting NOT cyTOF),Automating the removal of dead and duplicated cells
FACS
2 days ago by
ccbb7aab4
▴ 20
0
votes
1
reply
162
views
PyMOL: how to list all selection names?
pymol
updated 5 days ago by
Wayne
★ 1.8k • written 5 days ago by
Ondina
▴ 90
0
votes
0
replies
108
views
High pvalues when using clusterProfiler for seurat
clusterProfiler
1 day ago by
Ahmed
• 0
0
votes
3
replies
327
views
RSubread DESeq2
RNA-seq
subread
differential-expression
deseq2
updated 5 days ago by
Ram
40k • written 14 days ago by
Beyza
• 0
0
votes
0
replies
128
views
cosine simialrity of mutation signature analysis
similarity
Mutational
analysis
signature
cosine
5 days ago by
probioquestioner
• 0
0
votes
0
replies
88
views
Why dbConnect GEOmetadb_demo.sqlite shows some information while dbConnect GEOmetadb.sqlite shows no information
R
GEO
GEOmetadb
updated 1 day ago by
Pierre Lindenbaum
157k • written 1 day ago by
nonaddldy
▴ 10
0
votes
3
replies
241
views
How to find target genes From RNA seq data?
Cotton
DGE
RNA-seq
updated 4 days ago by
Ram
40k • written 4 days ago by
Fizzah
▴ 30
0
votes
0
replies
175
views
What metrics to use to calculate variant library evenness or uniformity or bias
library-bias
variant
updated 6 days ago by
Ram
40k • written 7 days ago by
eli_bayat
▴ 90
89 results • Page
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Comment: Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempti
Comment: Error in bowtie2
Recommended Tools For Alternative Splicing Detection From Rna-Seq Data
Comment: Using STAR aligner to build index of hg38
Answer: How to sort using samtools
The Biostar Handbook. A bioinformatics e-book for beginners.
The Biostar Handbook. A bioinformatics e-book for beginners.
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Comment: Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempti
by
Y
• 0
I tried the commands you recommended in the bash/command line. When I tried `find "${bam_directory}" -type f -name "*.bam" | xargs samtools…
Comment: Hugo_Symbol to Entrez ID
by
GenoMax
134k
Can you provide some examples of HUGO ID's you are unable to convert?
Comment: Using STAR aligner to build index of hg38
by
GenoMax
134k
GTF file includes gene models/annotation information. You could also use a program like `salmon/kallisto` with just human transcriptome to…
Comment: Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempti
by
Pierre Lindenbaum
157k
then test each files. find "${bam_directory}" -type f -name "*.bam" | xargs samtools quickcheck file "${reference_gen…
Answer: Ties in reranked list
by
alserg
▴ 840
The problem here is not the ties, but that your gene IDs in the pathway list (ensemble human genes) does not match the names of the stats v…
Comment: Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempti
by
Y
• 0
I did not use the space between the `-u` flag and the `-o` flag (the blank line) but I did do what you recommended: # Run CuffDiff wit…
Answer: Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempti
by
Pierre Lindenbaum
157k
how about simply trying: ``` cuffdiff -u "${annotation_gtf}" \ -o "${output_directory}" \ -b "${reference_genome}" \ -p "${num_th…
Comment: Using STAR aligner to build index of hg38
by
Grace
• 0
Yes, I know also need a GTF file. So the GTF file will include RNA information? Thanks for your answer!!
Comment: STAR Intron Motif Script Gives Segmentation fault Error
by
Y
• 0
I will try and figure it out on my own given what you all have mentioned. Thank you for your time.
Comment: Using STAR aligner to build index of hg38
by
Amitm
★ 2.2k
Have you gone through the manual [here][1]. In addition to the genome fasta file, you would also need a GTF file (of gene annotations) to b…
Comment: Highly inflated p-values in GWAS by regenie
by
4galaxy77
2.8k
You still definitely need to include principle components as covariates, even if your data is from the same ethnicity. Not including those …
Answer: How to create structural variants ground truth for alignment of two long-read ge
by
Christophe
• 0
Hi, D-genies is using minimap2 to align both genomes and minimap2 is chaining local alignments to produce a global one. If the SV are sm…
Comment: Ties in reranked list
by
Hamza
• 0
In this case there are no results in my fgsea object unfortunately
Comment: Genes with promoter and enhancer regions as GTF
by
abis.1819104
• 0
how to generate promoters.bed and enhancer.bed files from gtf file
Comment: Deepvariant variant calling by singularity
by
jeffhsu3
• 0
I am having a similar issue was this ever resolved? Getting this error: I0922 22:22:08.794076 140356687390528 make_examples_core.py:257] O…
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