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503 results • Page
4 of 11
Sort: Rank
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Votes
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0
votes
0
replies
152
views
Problem with GATK FilterMutectCalls : using contamination table doesn' t change anything
vcf
contaminationtable
gatk
filtermutectcalls
genomics
13 days ago by
Samuel
▴ 20
0
votes
1
reply
214
views
How do I know whether it is not a failure of ChIPseq data
ChIPseq
updated 10 days ago by
dthorbur
★ 1.9k • written 13 days ago by
Herman Einstein
• 0
0
votes
0
replies
138
views
CIBERSORT deconvolution by using long read sequencing data
long-read-sequencing
CIBERSORT
updated 12 days ago by
Ram
43k • written 13 days ago by
IVORY
• 0
0
votes
0
replies
152
views
Saccharomyces cerevisiae orthologs in plants
database
orthologs
13 days ago by
dec986
▴ 370
0
votes
0
replies
135
views
Looking for Comprehensive Virtual Screening Examples with Results Validation
drug-discovery
virtual-screening
molecular-docking
updated 13 days ago by
Ram
43k • written 13 days ago by
Stefano
• 0
0
votes
1
reply
170
views
samflags to removes mapped concordantly >1 times
samtools
Bowtie2
Metagenome
updated 7 days ago by
jkbonfield
★ 1.2k • written 13 days ago by
pompam_5904
• 0
3
votes
2
replies
209
views
Trying to write bwa mem -> samtools view -> samtools sort loop
bwa
samtools
mapping
genome
updated 13 days ago by
Pierre Lindenbaum
161k • written 13 days ago by
Lee
• 0
0
votes
0
replies
122
views
TransDecoder ORF score to filter "noise" from de novo transcriptome assembly
transdecoder
assembly
transcriptome
updated 13 days ago by
Ram
43k • written 13 days ago by
tdamiani
• 0
0
votes
0
replies
110
views
Should I keep taxa that have 0 counts when calculating alpha diversity?
Shannon-Weiner
13 days ago by
DNAngel
▴ 250
0
votes
5
replies
307
views
BUSCO analysis failed due to Duplicate of sequence in Input genome fasta. How to solve this problem ?
duplicate
BUSCO.
updated 13 days ago by
GenoMax
141k • written 13 days ago by
Sony
▴ 10
0
votes
1
reply
144
views
MMSeqs2 Removing Redundancy
mmseqs
mmseqs2
updated 13 days ago by
GenoMax
141k • written 13 days ago by
dthorbur
★ 1.9k
0
votes
0
replies
105
views
Transcription Factor Binding sites intersecting Transposable Elements
TE
TFBP
13 days ago by
M.Adiba
• 0
0
votes
5
replies
309
views
Incongruencies in GT assignment by Freebayes
depth
freebayes
heterozygous
genotype
13 days ago by
virginia.baraja
• 0
0
votes
0
replies
100
views
Extracting tcga images for cnn in r
rstudio
python
tcga-lihc
svs
14 days ago by
jain72744
▴ 10
0
votes
3
replies
202
views
Peptide identification from Mass spec data
Transcriptomics
Proteins
Mass-spec
updated 13 days ago by
Ram
43k • written 14 days ago by
atharvakarkare14
▴ 10
0
votes
7
replies
378
views
DESeq: too low p-value for RNAseq
R
DESeq
p-value
RNA-seq
FDR
updated 13 days ago by
Papyrus
★ 2.9k • written 14 days ago by
doramora
▴ 10
0
votes
0
replies
328
views
Looking to improve my understanding of Homer's mergePeaks function: unclear of how the merged file is created?
Homer
ATACseq
14 days ago by
Ronin
• 0
0
votes
0
replies
86
views
RobustRankAggreg rankMatrix subscript out of boundaries
RobustRankAggreg
microarray
R
rankMatrix
14 days ago by
hagl
▴ 10
0
votes
3
replies
260
views
How do I handle it if there are multiple ENSEMBL IDs in one gene symbol in the scRNA sequencing data?
Seurat
scRNA-seq
count-matrix
10 days ago by
Jeyong
• 0
0
votes
0
replies
77
views
Handling data from RNA-seq experiement with negative cell controls / Water samples
rnaseq
14 days ago by
kvn95ss
▴ 20
1
vote
1
reply
173
views
R/Python script to collect allele frequency for batch of SNPs in gnomAD database
gnomAD
13 days ago by
Shicheng Guo
★ 9.4k
0
votes
0
replies
95
views
Seurat-DE analysis
seurat
DE
14 days ago by
odi
▴ 10
0
votes
1
reply
141
views
sorted bam file generated from gff transcript fastas, how to get counts with HTseq?
htseq
bam
updated 14 days ago by
Ram
43k • written 14 days ago by
RNAseqer
▴ 260
1
vote
5
replies
321
views
samtools filtering
samtools
updated 7 days ago by
jkbonfield
★ 1.2k • written 14 days ago by
sansan_96
▴ 80
0
votes
0
replies
98
views
R/Python function to retrieve interacted proteins from HPA database
HPA
14 days ago by
Shicheng Guo
★ 9.4k
0
votes
0
replies
104
views
Extracting Enhancer Regions from ENCODE Annotation Files
ENCODE
enhancer
14 days ago by
YamengWu
• 0
0
votes
0
replies
93
views
Normalization approaches of RNAseq data before WGCNA
DEseq2
voom
WGCNA
14 days ago by
gimenagomez
• 0
0
votes
6
replies
434
views
Error in running Mauve alignner in Ubuntu 20.04
java
linux
aligner
mauve
8 days ago by
marongiu.luigi
▴ 710
0
votes
2
replies
208
views
RNA-Seq isoforms identification
RNA-seq
updated 14 days ago by
Andres
▴ 20 • written 14 days ago by
latita.atun
• 0
2
votes
2
replies
240
views
Removing adapters in all the RNAseq samples regardless if they have or not, is it correct?
fastqc
trimming
adapters
cutadapt
RNAseq
updated 14 days ago by
dsull
★ 5.8k • written 14 days ago by
ev97
▴ 20
0
votes
0
replies
96
views
ChIPseeker annotation on gene or transcript level
ChIPseeker
annotation
ChIP-seq
14 days ago by
zea
• 0
0
votes
0
replies
101
views
Integration of scRNA, a conceptual doubt
Seurat
scRNA
Integration
14 days ago by
edoardofilippi1998
• 0
0
votes
0
replies
127
views
Diffbind and DESeq2 comparison for differential sites
diffbind
deseq2
updated 8 days ago by
Ram
43k • written 14 days ago by
Ankit
▴ 500
0
votes
0
replies
145
views
Requesting Insights on Potential Complex Structural Event Indicated by IGV
Manta
IGV
variant
translocation
structural
15 days ago by
pragnapcu
• 0
0
votes
2
replies
198
views
utr sequence retrieval from UCSC
UTR
SEQUENCING
UCSC
GENOME
updated 15 days ago by
GenoMax
141k • written 15 days ago by
kim
▴ 70
0
votes
0
replies
88
views
NicheNet cell-cell communication analysis outcome
nichenet
scRNA-seq
15 days ago by
QX
• 0
0
votes
1
reply
152
views
mirbase gff and mature fastas for custom STAR index genome?
mirbase
indexing
star
genome
14 days ago by
RNAseqer
▴ 260
0
votes
0
replies
97
views
WGCNA modulePreservation on three expression sets
WGCNA
15 days ago by
CTLong
▴ 110
0
votes
1
reply
186
views
The order of those tied genes will be arbitrary, which may produce unexpected results.
fgsea
15 days ago by
Gordon
• 0
0
votes
0
replies
124
views
Problem in merging multiple samples VCF file obtained from Structural variant caller Lumpy
SV
Variant-calling
VCF
updated 15 days ago by
Ram
43k • written 15 days ago by
sardana.thakur.prachi
• 0
0
votes
0
replies
97
views
How to extract BED-format annotation tracks for the newly inserted segments
bed
mauve
updated 15 days ago by
Ram
43k • written 15 days ago by
FZ
• 0
0
votes
0
replies
102
views
Variant Calling for one gene - Stage of filtering
gatk
picard
variant-calling
updated 15 days ago by
Ram
43k • written 15 days ago by
andrebolerbarros
• 0
3
votes
5
replies
390
views
Very low RNA splicing rate for pulmonary AT2 cells
RNA-velocity
scVelo
scRNA-seq
splicing
updated 15 days ago by
GenoMax
141k • written 15 days ago by
e.r.zakiev
▴ 190
0
votes
0
replies
109
views
How to improve batch correction
ruvg
batch-correction
rna-seq
updated 15 days ago by
Ram
43k • written 15 days ago by
Shaimaa Gamal
▴ 10
0
votes
0
replies
93
views
WGCNA: analysis of complex trait associations with regression
WGCNA
15 days ago by
w.cookson
• 0
4
votes
9
replies
540
views
variant calling
variant-calling
updated 14 days ago by
Ram
43k • written 15 days ago by
dalibenam64
• 0
0
votes
0
replies
242
views
What does it means, when 2-direction perturbed pathways are identified in the gage enrichment analysis?
r
pathway
go-enrichment
gage
15 days ago by
Assa Yeroslaviz
★ 1.8k
1
vote
1
reply
165
views
Batch effect issue in RNA velocity analysis
RNA-velocity
integration
updated 16 days ago by
ATpoint
81k • written 16 days ago by
sarahmanderni
▴ 100
0
votes
4
replies
307
views
Compute bootstrap values on consensus tree
phylogeny
tree
raxml
2 days ago by
pablo
▴ 300
2
votes
1
reply
160
views
multiple reference sequences
freebayes
updated 16 days ago by
GenoMax
141k • written 16 days ago by
emilydolivo97
▴ 10
503 results • Page
4 of 11
Recent Votes
Answer: differential gene expression analysis when not all samples have an untreated cou
Answer: differential gene expression analysis when not all samples have an untreated cou
Answer: Sheep reference genome remapping coordinates
Answer: Sheep reference genome remapping coordinates
DEG analysis of RNA-seq data across multiple tissues and two conditions
Answer: Assume "yes" as the answer to any prompts with bioconda
Answer: Assume "yes" as the answer to any prompts with bioconda
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Recent Awards •
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141k
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Recent Replies
Comment: Sheep reference genome remapping coordinates
by
valentinatsar
• 0
Thank you for your response As I can understand this tool takes sequence input files right? I have genome-wide genotypes instead of sequen…
Comment: How can I retrieve wheat lysine non-acetylated sequences from the UniProt databa
by
me
▴ 750
Ok I made this into an answer. Could you please accept it as such.
Answer: How can I retrieve wheat lysine non-acetylated sequences from the UniProt databa
by
me
▴ 750
You can't. The data does not exist. UniProt only annotates where a lysine is know to be acetylated. There is no way that UniProt can know w…
Comment: How to compare the quality of assemblies
by
GenoMax
141k
Are there any short read datasets available in SRA? Can you try and see how many left over reads remain after you align to both of your ass…
Comment: Korean human genome reference file
by
GenoMax
141k
From NCBI: https://www.ncbi.nlm.nih.gov/datasets/genome/GCA_001712695.1/ Use the `Download` button or `curl/datasets`.
Comment: SLURM submission problem: Indexing the Reference Genome is not done in STAR
by
GenoMax
141k
> But it keeps giving error Please tell us what the error is.
Answer: 10x VDJ (TCR/BCR) BAM to fastq
by
GenoMax
141k
Looking at the information about the BAM file in the link included at the end of this sentence, it seems that the BAM file does not contain…
Comment: How can I retrieve wheat lysine non-acetylated sequences from the UniProt databa
by
Jacky
• 0
I got it, thank you for your response.
Answer: DEseq2
by
ATpoint
81k
The DESeq2 input is a numeric count matrix (or data.frame) with rows being genes/observations and columns being samples/cells/specimen. Ple…
Comment: How to add motifs in cellranger-arc reference?
by
Ehsan
• 0
Hi. Do you have any updates on what you ended up doing? I am encountering same issue, cannot really find a good TF motif database for zebra…
Comment: biomaRt error for different R version
by
manaswwm
▴ 490
Wonderful, and thanks for tagging Mike! For those who come back for this problem, the following seemed to work for me: Downgrading `dbply…
Answer: Assume "yes" as the answer to any prompts with bioconda
by
Juke34
8.5k
From my experience using `biocontainers` base container no need to set `yes` installation is done without any command prompt, otherwise as …
Comment: SLURM submission problem: Indexing the Reference Genome is not done in STAR
by
Dave Carlson
★ 1.7k
You're allocating 4 compute nodes (`#SBATCH -N 4`), but STAR can't actually make use of more than 1 compute node. You can increase the num…
Answer: Assume "yes" as the answer to any prompts with bioconda
by
Dave Carlson
★ 1.7k
```conda install -y bioconda::packageX conda-forge::packageY``` should do what you're looking for.
Comment: Supergnova stuck in tutorial
by
Murat
• 0
Hi GenoMax, Thank you for your advice. I sent an email to them and have already opened an issue on Github. Let see if there is any other so…
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