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2
votes
2
answers
109
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5 follow
2
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To reassemble Illumina and PacBio, or just upgrade previous assembly with PacBio?
genome assembly pacbio illumina written 2 days ago by msobol • 10 • updated 1 day ago by colindaven • 740
27
votes
2
answers
3.3k
views
24 follow
2
answers
Tutorial: Polish PacBio assembly with latest PacBio tools : an affordable solution for everyone
pbalign tutorial polishing pacbio arrow smrt limk written 10 months ago by Roxane Boyer • 690 • updated 1 day ago by Vijay Lakhujani ♦ 2.8k
3
votes
1
answer
126
views
7 follow
1
answer
How to perform quality check and assembly using pacbio sequel data?
sequel assembly qc error correction pacbio written 3 days ago by Vijay Lakhujani ♦ 2.8k
5
votes
2
answers
97
views
2
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Falcon - Estimation of free disc space needed
assembly falcon pacbio written 9 days ago by alslonik • 20
5
votes
3
answers
223
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6 follow
3
answers
De novo plant genome assembly
genome assembly chromium falcon pacbio written 20 days ago by alslonik • 20
0
votes
0
answers
95
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0
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PacBio assemblies only ending up somewhere between 80 and 250 contigs.
de novo assembly pacbio written 10 days ago by dylan.lawrence • 10
0
votes
2
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321
views
2
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Arrow Assembly polishing Error "KeyError: 'BASECALLERVERSION'"
arrow pacbio basecallerversion assembly written 4 months ago by David_emir • 290 • updated 13 days ago by whm568019240 • 0
0
votes
0
answers
87
views
0
answers
Phylogenetic tree for pooled PACBio sequencing material
phylogeny pooled msa pacbio written 25 days ago by a.m.dekker • 0
0
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0
answers
87
views
0
answers
Issues running pbsmrtpipe and confirmation dialogue
pacbio written 26 days ago by dylan.lawrence • 10
2
votes
0
answers
673
views
0
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How low abundance can PacBio full length RNAseq can detect
pacbio rna-seq written 21 months ago by pengchy • 400 • updated 27 days ago by Biostar ♦♦ 20
0
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0
answers
100
views
0
answers
n50 contig with pacbio nowadays ?
n50 pacbio written 7 weeks ago by Picasa • 350
0
votes
0
answers
151
views
0
answers
Subset BAM file based on selected coverage, PacBio reads
arrow pbalign coverage bam pacbio written 7 weeks ago by MyrNat • 0
2
votes
1
answer
165
views
7 follow
1
answer
Decision on no. and type of libraries to be generated for plant genome assembly
genome assembly library pacbio illumina written 9 weeks ago by MSM55 • 70
5
votes
1
answer
171
views
1
answer
PacBio methylation information in bam file
methylation bam samtools pacbio written 9 weeks ago by gabri • 30
0
votes
0
answers
145
views
0
answers
falcon unzip output
falcon pacbio written 9 weeks ago by rob234king • 550
9
votes
0
answers
932
views
5 follow
0
answers
Tool: NanoPack: Scripts for long read sequencing data processing and analysis: NanoPlot, NanoFilt, NanoStat, NanoLyse, NanoComp
long reads tool pacbio oxford nanopore written 10 months ago by WouterDeCoster ♦ 30k
0
votes
0
answers
105
views
0
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which sequence file should be transcripts in full-length transcriptome analysis
full-length transcriptome pacbio transcripts written 11 weeks ago by fanglujing • 30
0
votes
0
answers
242
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0
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Job: Three years Postdoctoral position in Plant/Fungal Genomics - University of Ottawa (Canada)
assembly genomics job pacbio fungal plant written 12 weeks ago by salmo7777 • 0
2
votes
1
answer
198
views
1
answer
How to use pacbio reads to join contigs
tgs assembly long pacbio written 3 months ago by 934963534 • 10 • updated 3 months ago by h.mon ♦ 16k
2
votes
0
answers
224
views
0
answers
Minimum coverage for PacBio Arrow
assembly polishing pacbio arrow written 3 months ago by jean.elbers • 290
1
vote
0
answers
186
views
5 follow
0
answers
PacBio Genome assembly
genome assembly alignment pacbio gene written 3 months ago by julienlevy • 0 • updated 3 months ago by genomax ♦ 52k
3
votes
2
answers
1.2k
views
5 follow
2
answers
[PacBio assembly] Remaining indels after polishing
assembly polishing pacbio quiver written 13 months ago by Roxane Boyer • 690 • updated 4 months ago by Biostar ♦♦ 20
2
votes
1
answer
252
views
1
answer
The orientation of Pacbio read.
tgs fastq longread pacbio written 4 months ago by 934963534 • 10 • updated 4 months ago by h.mon ♦ 16k
0
votes
1
answer
307
views
1
answer
PacBio long read error correction tools
long-read pacbio error-correction short-read written 4 months ago by freddiejung • 20 • updated 4 months ago by lieven.sterck • 1.9k
4
votes
1
answer
572
views
5 follow
1
answer
Pipeline for hybrid genome assembly of long and short reads AND how to deal with PacBio sequel data
genome illumina hybrid assembly pacbio assembly written 4 months ago by freddiejung • 20 • updated 4 months ago by Carambakaracho • 360
1
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0
answers
211
views
0
answers
Huge jump in plasmid coverage - any idea why?
pilon illumina bacteria pacbio written 4 months ago by predeus • 590
0
votes
0
answers
185
views
0
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Get aligment quality / identity from CRAM file (for PacBio and ONT data)
cram pacbio alignment quality ont written 4 months ago by mschmid • 90
1
vote
1
answer
171
views
1
answer
PacBio sequence lengths distribution
sequence pacbio sequencing written 4 months ago by Ric • 180 • updated 4 months ago by WouterDeCoster ♦ 30k
0
votes
0
answers
442
views
0
answers
falcon-unzip installation and testing using pacbio data
falcon pacbio written 4 months ago by rob234king • 550
1
vote
1
answer
175
views
1
answer
Help with nhmmscan on PacBio WGS Data with Dfam
wgs nhmmscan dfam hmm pacbio written 5 months ago by roxane.dunbar • 0
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