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Tutorial: Polish PacBio assembly with latest PacBio tools : an affordable solution for everyone

pbalign tutorial polishing pacbio arrow smrt limk written 27 days ago by Roxane Boyer • 380 • updated 13 days ago by zusman • 0

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Help Needed with Pbalign SamFIlter

quiver pbalign pacbio samfilter written 10 months ago by anniiee9 • 0 • updated 9 months ago by Biostar ♦♦ 20

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299

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samFilter Shows CoreDump

quiver pbalign samfilter written 10 months ago by anniiee9 • 0

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359

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pbalign - SortService returned a non-zero exit status

assembly pbalign pacbio written 11 months ago by mjp • 10 • updated 11 months ago by Biostar ♦♦ 20

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pbalign issue: blasr v5.1 seems to be not integrated with pbalign

alignment pbalign blasr pacbio written 13 months ago by Pawel Osipowski • 20

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reference sequence for blasr and pbalign

de novo assembly pbalign blasr pacbio written 20 months ago by komwit.s • 0

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Recent Replies

C: align_and_estimate_abundance after assembly in Trinity by genomax ♦ 34k
If you did, then describe it here in detail, instead posting this cryptic line. It would be usefu...
A: align_and_estimate_abundance after assembly in Trinity by nasreenbano91 • 0
find the solution.
C: denovo assembly of unmapped reads by genomax ♦ 34k
Provided you have an adequate amount of reads left over to do a reasonable assembly. What fractio...
C: Network algorithms & metrics by LLTommy • 990
I don't know what kind of paper you are looking for. There are many different network algorithms ...
A: literature review by a web server by genomax ♦ 34k
- [Open targets platform][1] from EMBL-EBI is your best free option. - [OMIM][2] is focused on ...
C: Interview Dealing with RADseq data: some tips from our instructors by carlopecoraro2 • 540
Many thanks Istvan! Cheers
C: Subtracting one BAM [control] from another BAM [sample]. by Pierre Lindenbaum ♦ 99k
add one sample must be named "control" to fit with 'snippet.txt'
C: Subtracting one BAM [control] from another BAM [sample]. by Pierre Lindenbaum ♦ 99k
your two bams are missing a **RG** tag with a sample name to tell which 'rea'd is control, which ...
C: NGS statistics - Mean coverage and % of bases >20x (or probability to miss one o by Kevin Blighe • 3.0k
Hey, yes, I wish that it was simple (but as you've implied, it's not!). Just to be sure: when yo...
C: NGS statistics - Mean coverage and % of bases >20x (or probability to miss one o by Kevin Blighe • 3.0k
This is read-depth at each variant position in the genome. It's just reporting sensitivity (not s...
A: bowtie -n aligment mode by glihm • 400
Hello valdirbarth, You have the good understanding of the **-n** and **-l** options. However, th...
A: denovo assembly of unmapped reads by Macspider • 1.5k
I think the best way to proceed would be to: - map your RNASeq reads against the wheat genome w...

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