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Comment: Redirection of Duplicate PMIDs
Answer: Redirection of Duplicate PMIDs
A: Should I Remove All Positions Containing A Gap In A Multiple Alignment Prior To
Should I Remove All Positions Containing A Gap In A Multiple Alignment Prior To Creating A Phylogenetic Tree?
Answer: Is it Acceptable to Have Uniform Quality Scores in a FASTQ File?
Comment: Installing feature counts package in command line
Comment: Marking duplicates using UMIs
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Recent Replies
Comment: Should I use unpaired reads from trimmomatic
by
swbarnes2
14k
If the unpaired are _2, files, the _1 must exist somewhere. But the simple answer is probably going to be to just use all the _2 only. Yo…
Comment: Is it Acceptable to Have Uniform Quality Scores in a FASTQ File?
by
GenoMax
141k
> The needless granularity (fake precision) was obvious from the start You are assuming that all data generated is always of great quality…
Answer: Blasting two protein sequences vs two nucleotide sequences
by
Istvan Albert
100k
There are various versions of blast, that search in translated spaces, see blastx and tblastn https://blast.ncbi.nlm.nih.gov/Blast.cgi …
Answer: Should I use unpaired reads from trimmomatic
by
Istvan Albert
100k
If you have lots of data, both paired and unpaired, then the best strategy is probably to treat them separately and merge the counts at the…
Comment: Difference between samtools "-f 9" and "-f 11"
by
Istvan Albert
100k
It is very easy to make nonsensical flags (unfortunately a flaw of the SAM spec itself) ... not to mention the awkward constructs: a primar…
Comment: Heatmap and rna-seq
by
Istvan Albert
100k
This forum is dedicated to asking their question on bioinformatics. As long as the topic is bioinformatics, all questions are welcome, and …
Comment: Is it Acceptable to Have Uniform Quality Scores in a FASTQ File?
by
Istvan Albert
100k
I interpret this as an admission by SRA that phred scores are practically useless. The needless granularity (fake precision) was obvious f…
Comment: TCGA2STAT Error: Firehose connection
by
Ram
43k
If their github repo is alive, raise an issue there.
Comment: TCGA2STAT Error: Firehose connection
by
Ngrin
• 0
Yeah makes sense. I was following their github. Thanks for pointing this out.
Comment: The Biostar Herald for Tuesday, April 23, 2024
by
Pierre Lindenbaum
161k
bwa 0.7.18 released ! https://github.com/lh3/bwa/releases/tag/v0.7.18
Comment: Downsampling fastq file
by
Ram
43k
I'm moving this to a comment for now.
Answer: Seurat merge and batch correction
by
bk11
★ 2.3k
You can merge your seven Seurat objects and perform integration using **Seurat V5** to remove the batch effect- ```r library(Seurat) libra…
Comment: How do I use the STARSolo aligner with MGI DNBelab C series HT scRNAseq librarie
by
atowns21
• 0
Yeah, I've been digging around the web for a while now. Can't find any information about the barcodes. The total number of combinations is …
Comment: How do I use the STARSolo aligner with MGI DNBelab C series HT scRNAseq librarie
by
GenoMax
141k
Ah I see that in now. I am not sure why MGI has split the whitelist into two sections. You may need to see if you can dig up some info abou…
Comment: How do I use the STARSolo aligner with MGI DNBelab C series HT scRNAseq librarie
by
atowns21
• 0
read 1 is 30bp... which I think you meant to type. If you scroll approximately halfway down the json file I linked to, it has the following…
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