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qPCR gene expression confusion
gene rna qpcr rna-seq written 7 weeks ago by muha0185 • 0 • updated 7 weeks ago by ATpoint ♦ 21k
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177
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Confounding factors when comparing effects of expression between RNA-seq samples and qPCR samples?
qrtpcr transcriptomics rna-seq qpcr expression written 3 months ago by michael.nagle • 100 • updated 3 months ago by Devon Ryan ♦ 91k
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254
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News: eDNA Metabarcoding Workshop in Berlin
metabarcoding edna news qpcr written 4 months ago by carlopecoraro2 • 1.2k
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Job: Senior Scientific Officer
digitalpcr bioplex job qpcr pcr written 8 months ago by akhilvbioinfo • 140 • updated 8 months ago by WouterDeCoster ♦ 40k
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How to perform PCA on gene expression?
marker gene gene expression pca qpcr clustering written 9 months ago by Thomas B. • 10 • updated 9 months ago by ahmad mousavi • 450
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190
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How to deal with inosines in primer sequences?
rt-pcr qpcr pcr written 10 months ago by dom.byrne13 • 20
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6 follow
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How to design qPCR primers to validate RNA-seq experiment?
rna-seq qpcr written 11 months ago by brett.vanderwerff • 20 • updated 11 months ago by Malcolm.Cook • 1.1k
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416
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Analyzing fold change when Cq value in treatment condition is 0 (not expressed)
qpcr written 11 months ago by jajarosli • 10 • updated 11 months ago by Devon Ryan ♦ 91k
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7 follow
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How to "simulate" a qPCR reaction in silico?
primer verification primers qpcr written 11 months ago by dom.byrne13 • 20
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How to calculate Log2 Fold Change for normalised qPCR data?
qpcr written 2.7 years ago by Genosa • 100 • updated 11 months ago by Kevin Blighe ♦ 46k
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How to report and plot qPCR data
qpcr fold change written 3.1 years ago by sabela • 0 • updated 12 months ago by Kevin Blighe ♦ 46k
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832
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qPCR: Huge variation in fold change of genes between biological replicates
foldchange qpcr written 14 months ago by Ambika • 30 • updated 14 months ago by WouterDeCoster ♦ 40k
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5 follow
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How to statistically compare multiple cell lines
R qpcr written 16 months ago by elb • 160 • updated 16 months ago by Devon Ryan ♦ 91k
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791
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Primer design to do qPCR on repetitive elements
repeats repetitive elements qpcr written 19 months ago by Gema Sanz • 70 • updated 19 months ago by trausch • 1.4k
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764
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1
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Correct for confounding variable (variability in knockdown efficiency) in paired t-test for change in gene expression
t-test gene ancova qpcr statistics written 19 months ago by Scott • 80 • updated 19 months ago by Devon Ryan ♦ 91k
2
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1
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516
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1
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Multiplex gene expression normalization
qpcr written 21 months ago by yuggnurug • 70 • updated 21 months ago by Kevin Blighe ♦ 46k
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1
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1
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Minimal TPM threshold for qPCR measure of gene expression
tximport tpm qpcr rna-seq kallisto written 21 months ago by tlorin • 250
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1
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998
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1
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primer desing for qPCR on de novo assembly output
rnaseq primer qpcr written 2.0 years ago by pablo61991 • 70 • updated 23 months ago by Kevin Blighe ♦ 46k
2
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1
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787
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1
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Design qpcr primers
primers sequence rna-seq qpcr written 2.0 years ago by sbchua.1990 • 30 • updated 2.0 years ago by James Ashmore • 2.6k
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647
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Best approach to quantify specific bacteria strain
sequence alignment qpcr snp written 2.1 years ago by CY • 370
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838
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qPCR to validate gene FPKM is 0 in RNA-Seq
rna-seq qpcr written 2.3 years ago by l0o0 • 190
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505
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Different calibrator samples in 2 qPCRs- statistics issue
calibrator qpcr statistics written 2.4 years ago by V24 • 0 • updated 2.3 years ago by Biostar ♦♦ 20
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3
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998
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3
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Statistics for qPCR with varying number of samples between groups
patient samples qpcr statistics written 2.8 years ago by V24 • 0
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1
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1
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Quantifying relative ratios of variants by qPCR
qpcr deltadeltact written 2.8 years ago by ThePresident • 130 • updated 2.8 years ago by hazzardwallace • 0
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Allele-specific quantification methods for short tandem repeats?
allele microsatellite qpcr pcr written 2.8 years ago by michealsmith • 740
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GSEA for qPCR data
go qpcr gsea written 3.4 years ago by peter pfand • 80 • updated 2.8 years ago by Biostar ♦♦ 20
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Is qPCR actually required for RNA-Seq validation ?
rna-seq qpcr validation written 2.9 years ago by Antonio R. Franco • 4.1k • updated 2.8 years ago by Devon Ryan ♦ 91k
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DIY RNA Spike-in controls for qRTPCR
rtpcr rna spike-in qpcr written 3.0 years ago by samantha_jeschonek • 50 • updated 2.9 years ago by Biostar ♦♦ 20
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Query regarding geNorm in NormqPCR package of R?
R genorm qpcr normqpcr written 2.9 years ago by kamran.shekh • 10 • updated 2.9 years ago by guto03 • 0
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Pretty display of values for samples arranged in a 384-well plate (in R).
R 384-well plate 96-well plate qpcr plot written 3.4 years ago by Charles Plessy • 2.7k • updated 3.1 years ago by S.Warchal • 30
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