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<prev • 31 results • page 1 of 2 • next >
4
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168
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6 follow
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How to design qPCR primers to validate RNA-seq experiment?
rna-seq qpcr written 11 days ago by brett.vanderwerff • 10 • updated 2 days ago by Malcolm.Cook • 820
3
votes
1
answer
81
views
1
answer
Analyzing fold change when Cq value in treatment condition is 0 (not expressed)
qpcr written 5 days ago by jajarosli • 10 • updated 5 days ago by Devon Ryan ♦ 84k
1
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0
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108
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7 follow
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How to "simulate" a qPCR reaction in silico?
primer verification primers qpcr written 8 days ago by dom.byrne13 • 20
1
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2
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9.3k
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2
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How to calculate Log2 Fold Change for normalised qPCR data?
qpcr written 21 months ago by Genosa • 100 • updated 26 days ago by Kevin Blighe ♦ 28k
4
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1
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5.4k
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1
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How to report and plot qPCR data
qpcr fold change written 2.2 years ago by sabela • 0 • updated 5 weeks ago by Kevin Blighe ♦ 28k
0
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0
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190
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0
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qPCR: Huge variation in fold change of genes between biological replicates
foldchange qpcr written 3 months ago by Ambika • 20 • updated 3 months ago by WouterDeCoster ♦ 32k
1
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0
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231
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5 follow
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How to statistically compare multiple cell lines
R qpcr written 5 months ago by elb • 120 • updated 5 months ago by Devon Ryan ♦ 84k
1
vote
1
answer
414
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1
answer
Primer design to do qPCR on repetitive elements
repeats repetitive elements qpcr written 8 months ago by Gema Sanz • 50 • updated 8 months ago by trausch • 1.1k
1
vote
1
answer
394
views
1
answer
Correct for confounding variable (variability in knockdown efficiency) in paired t-test for change in gene expression
t-test gene ancova qpcr statistics written 8 months ago by Scott • 70 • updated 8 months ago by Devon Ryan ♦ 84k
2
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1
answer
341
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1
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Multiplex gene expression normalization
qpcr written 10 months ago by yuggnurug • 70 • updated 10 months ago by Kevin Blighe ♦ 28k
2
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1
answer
882
views
1
answer
Minimal TPM threshold for qPCR measure of gene expression
tximport tpm qpcr rna-seq kallisto written 10 months ago by tlorin • 240
2
votes
1
answer
576
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1
answer
primer desing for qPCR on de novo assembly output
rnaseq primer qpcr written 13 months ago by pablo61991 • 40 • updated 12 months ago by Kevin Blighe ♦ 28k
2
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1
answer
517
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1
answer
Design qpcr primers
primers sequence rna-seq qpcr written 13 months ago by sbchua.1990 • 20 • updated 13 months ago by James Ashmore • 2.5k
2
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0
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406
views
0
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Best approach to quantify specific bacteria strain
sequence alignment qpcr snp written 14 months ago by CY • 190
0
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0
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553
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0
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qPCR to validate gene FPKM is 0 in RNA-Seq
rna-seq qpcr written 17 months ago by l0o0 • 150
0
votes
0
answers
358
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0
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Different calibrator samples in 2 qPCRs- statistics issue
calibrator qpcr statistics written 18 months ago by V24 • 0 • updated 17 months ago by Biostar ♦♦ 20
0
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3
answers
794
views
3
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Statistics for qPCR with varying number of samples between groups
patient samples qpcr statistics written 23 months ago by V24 • 0
0
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1
answer
804
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1
answer
Quantifying relative ratios of variants by qPCR
qpcr deltadeltact written 22 months ago by ThePresident • 100 • updated 22 months ago by hazzardwallace • 0
0
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0
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558
views
0
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Allele-specific quantification methods for short tandem repeats?
allele microsatellite qpcr pcr written 23 months ago by michealsmith • 740
0
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1
answer
847
views
1
answer
GSEA for qPCR data
go qpcr gsea written 2.5 years ago by peter pfand • 70 • updated 23 months ago by Biostar ♦♦ 20
11
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1
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3.1k
views
1
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Is qPCR actually required for RNA-Seq validation ?
rna-seq qpcr validation written 23 months ago by Antonio R. Franco • 3.8k • updated 23 months ago by Devon Ryan ♦ 84k
3
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0
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1.5k
views
0
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DIY RNA Spike-in controls for qRTPCR
rtpcr rna spike-in qpcr written 2.1 years ago by samantha_jeschonek • 50 • updated 23 months ago by Biostar ♦♦ 20
0
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1
answer
898
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1
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Query regarding geNorm in NormqPCR package of R?
R genorm qpcr normqpcr written 2.0 years ago by kamran.shekh • 10 • updated 23 months ago by guto03 • 0
14
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2
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2.0k
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2
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Pretty display of values for samples arranged in a 384-well plate (in R).
R 384-well plate 96-well plate qpcr plot written 2.5 years ago by Charles Plessy • 2.5k • updated 2.2 years ago by S.Warchal • 30
0
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2
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900
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2
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Differential expression in qPCR Data after filtering
qpcr differential expression written 2.4 years ago by peter pfand • 70 • updated 2.4 years ago by Devon Ryan ♦ 84k
2
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0
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1.2k
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0
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Is there a good site or software to see if a primer pair spans an exon junction?
primers exons qpcr pcr written 3.1 years ago by gaelgarcia • 100
0
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1
answer
2.0k
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1
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Analysis of qPCR data
qpcr clustering written 3.2 years ago by simonhb1990 • 20 • updated 3.2 years ago by Devon Ryan ♦ 84k
0
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1
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2.3k
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1
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qpcR R package using output from ABI 7900HT (SDS 2.4)
R qpcr sds 7900ht written 4.1 years ago by piotr.grabowski • 50 • updated 3.5 years ago by heather.bouzek • 0
0
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1.2k
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0
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Format of Roche LightCycler output files *.ABT / *.CCC for colour compensation
qpcr crosstalk written 3.6 years ago by me • 0
0
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0
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1.5k
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0
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News: Total RNA Commercial Kit Comparison Study
ngs microarray news qpcr rna-seq written 3.8 years ago by andorjkiss • 10
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