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4
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1
answer
11k
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1
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How to report and plot qPCR data - delta delta Ct
qpcr fold change written 3.4 years ago by sabela • 0 • updated 26 days ago by Kevin Blighe ♦ 52k
1
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0
answers
69
views
0
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Add error bars to barplot that uses melted dataframe in R
R ggplot2 ddct qpcr written 4 weeks ago by j.lunger18 • 10
2
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0
answers
134
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0
answers
Advice on 16S Alignment for genus-specific qPCR design?
16s phylogenetics qpcr written 8 weeks ago by Tbr • 0
3
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0
answers
206
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0
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Job: Postdoc in computational and experimental annotation of tRNA-related sequences
genomics job trna qpcr plant hybridization written 12 weeks ago by a.zielezinski ♦ 8.9k
0
votes
1
answer
147
views
1
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Calculating ct values in qPCR for sample of timepoints to give a relative gene expression
δδct relative expression qpcr fold change written 3 months ago by rosie.little93 • 0 • updated 3 months ago by Eslam Samir • 100
1
vote
1
answer
242
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1
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qPCR gene expression confusion
gene rna qpcr rna-seq written 5 months ago by muha0185 • 0 • updated 5 months ago by ATpoint ♦ 26k
0
votes
1
answer
261
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1
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Confounding factors when comparing effects of expression between RNA-seq samples and qPCR samples?
qrtpcr transcriptomics rna-seq qpcr expression written 7 months ago by michael.nagle • 100 • updated 7 months ago by Devon Ryan ♦ 93k
0
votes
0
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343
views
0
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News: eDNA Metabarcoding Workshop in Berlin
metabarcoding edna news qpcr written 8 months ago by carlopecoraro2 • 1.3k
1
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0
answers
481
views
0
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Job: Senior Scientific Officer
digitalpcr bioplex job qpcr pcr written 12 months ago by akhilvbioinfo • 140 • updated 12 months ago by WouterDeCoster ♦ 42k
6
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2
answers
1.7k
views
2
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How to perform PCA on gene expression?
marker gene gene expression pca qpcr clustering written 13 months ago by Thomas B. • 10 • updated 13 months ago by ahmad mousavi • 450
0
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0
answers
238
views
0
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How to deal with inosines in primer sequences?
rt-pcr qpcr pcr written 14 months ago by dom.byrne13 • 20
4
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2
answers
1.1k
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6 follow
2
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How to design qPCR primers to validate RNA-seq experiment?
rna-seq qpcr written 15 months ago by brett.vanderwerff • 20 • updated 14 months ago by Malcolm.Cook • 1.1k
4
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1
answer
505
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1
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Analyzing fold change when Cq value in treatment condition is 0 (not expressed)
qpcr written 15 months ago by jajarosli • 10 • updated 15 months ago by Devon Ryan ♦ 93k
1
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0
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611
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7 follow
0
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How to "simulate" a qPCR reaction in silico?
primer verification primers qpcr written 15 months ago by dom.byrne13 • 20
3
votes
2
answers
14k
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2
answers
How to calculate Log2 Fold Change for normalised qPCR data?
qpcr written 3.0 years ago by Genosa • 100 • updated 15 months ago by Kevin Blighe ♦ 52k
0
votes
0
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1.0k
views
0
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qPCR: Huge variation in fold change of genes between biological replicates
foldchange qpcr written 18 months ago by Ambika • 30 • updated 18 months ago by WouterDeCoster ♦ 42k
1
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0
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606
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5 follow
0
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How to statistically compare multiple cell lines
R qpcr written 20 months ago by elb • 170 • updated 20 months ago by Devon Ryan ♦ 93k
1
vote
1
answer
891
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1
answer
Primer design to do qPCR on repetitive elements
repeats repetitive elements qpcr written 23 months ago by Gema Sanz • 70 • updated 23 months ago by trausch • 1.4k
1
vote
1
answer
876
views
1
answer
Correct for confounding variable (variability in knockdown efficiency) in paired t-test for change in gene expression
t-test gene ancova qpcr statistics written 23 months ago by Scott • 80 • updated 23 months ago by Devon Ryan ♦ 93k
2
votes
1
answer
590
views
1
answer
Multiplex gene expression normalization
qpcr written 2.1 years ago by yuggnurug • 70 • updated 2.1 years ago by Kevin Blighe ♦ 52k
2
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1
answer
1.8k
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1
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Minimal TPM threshold for qPCR measure of gene expression
tximport tpm qpcr rna-seq kallisto written 2.1 years ago by tlorin • 250
2
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1
answer
1.1k
views
1
answer
primer desing for qPCR on de novo assembly output
rnaseq primer qpcr written 2.3 years ago by pablo61991 • 70 • updated 2.3 years ago by Kevin Blighe ♦ 52k
2
votes
1
answer
886
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1
answer
Design qpcr primers
primers sequence rna-seq qpcr written 2.3 years ago by sbchua.1990 • 30 • updated 2.3 years ago by James Ashmore • 2.7k
2
votes
0
answers
731
views
0
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Best approach to quantify specific bacteria strain
sequence alignment qpcr snp written 2.4 years ago by CY • 460
0
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0
answers
920
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0
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qPCR to validate gene FPKM is 0 in RNA-Seq
rna-seq qpcr written 2.6 years ago by l0o0 • 190
0
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0
answers
559
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0
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Different calibrator samples in 2 qPCRs- statistics issue
calibrator qpcr statistics written 2.7 years ago by V24 • 0 • updated 2.6 years ago by Biostar ♦♦ 20
0
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3
answers
1.1k
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3
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Statistics for qPCR with varying number of samples between groups
patient samples qpcr statistics written 3.1 years ago by V24 • 0
0
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1
answer
1.3k
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1
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Quantifying relative ratios of variants by qPCR
qpcr deltadeltact written 3.1 years ago by ThePresident • 140 • updated 3.1 years ago by hazzardwallace • 0
0
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0
answers
755
views
0
answers
Allele-specific quantification methods for short tandem repeats?
allele microsatellite qpcr pcr written 3.1 years ago by michealsmith • 740
0
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1
answer
1.2k
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1
answer
GSEA for qPCR data
go qpcr gsea written 3.7 years ago by peter pfand • 80 • updated 3.2 years ago by Biostar ♦♦ 20
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