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0
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74
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qPCR results and enrichment analysis with comparison
gene cytoscape qpcr written 23 days ago by pavan.mathur • 0 • updated 16 days ago by scooter • 200
3
votes
2
answers
119
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2
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Correlation vs differential expression testing
qpcr written 6 weeks ago by prabin.dm • 170 • updated 6 weeks ago by mike-zx • 190
4
votes
1
answer
12k
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1
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How to report and plot qPCR data - delta delta Ct
qpcr fold change written 3.7 years ago by sabela • 0 • updated 4 months ago by Kevin Blighe ♦ 56k
1
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0
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137
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0
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Add error bars to barplot that uses melted dataframe in R
R ggplot2 ddct qpcr written 4 months ago by j.lunger18 • 10
2
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0
answers
188
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0
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Advice on 16S Alignment for genus-specific qPCR design?
16s phylogenetics qpcr written 5 months ago by Tbr • 0
3
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0
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269
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0
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Job: Postdoc in computational and experimental annotation of tRNA-related sequences
genomics job trna qpcr plant hybridization written 6 months ago by a.zielezinski ♦ 9.1k
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1
answer
200
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1
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Calculating ct values in qPCR for sample of timepoints to give a relative gene expression
δδct relative expression qpcr fold change written 6 months ago by rosie.little93 • 0 • updated 6 months ago by Eslam Samir • 100
1
vote
1
answer
316
views
1
answer
qPCR gene expression confusion
gene rna qpcr rna-seq written 9 months ago by muha0185 • 0 • updated 9 months ago by ATpoint ♦ 32k
0
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1
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334
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1
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Confounding factors when comparing effects of expression between RNA-seq samples and qPCR samples?
qrtpcr transcriptomics rna-seq qpcr expression written 11 months ago by michael.nagle • 100 • updated 11 months ago by Devon Ryan ♦ 94k
0
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0
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425
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News: eDNA Metabarcoding Workshop in Berlin
metabarcoding edna news qpcr written 12 months ago by carlopecoraro2 • 1.5k
1
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0
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529
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0
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Job: Senior Scientific Officer
digitalpcr bioplex job qpcr pcr written 16 months ago by akhilvbioinfo • 150 • updated 15 months ago by WouterDeCoster ♦ 43k
6
votes
2
answers
2.4k
views
2
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How to perform PCA on gene expression?
marker gene gene expression pca qpcr clustering written 17 months ago by Thomas B. • 10 • updated 17 months ago by ahmad mousavi • 470
0
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0
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280
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0
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How to deal with inosines in primer sequences?
rt-pcr qpcr pcr written 18 months ago by dom.byrne13 • 20
4
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2
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1.3k
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6 follow
2
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How to design qPCR primers to validate RNA-seq experiment?
rna-seq qpcr written 18 months ago by brett.vanderwerff • 20 • updated 18 months ago by Malcolm.Cook • 1.1k
4
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1
answer
620
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1
answer
Analyzing fold change when Cq value in treatment condition is 0 (not expressed)
qpcr written 18 months ago by jajarosli • 10 • updated 18 months ago by Devon Ryan ♦ 94k
1
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0
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726
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7 follow
0
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How to "simulate" a qPCR reaction in silico?
primer verification primers qpcr written 18 months ago by dom.byrne13 • 20
3
votes
2
answers
15k
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2
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How to calculate Log2 Fold Change for normalised qPCR data?
qpcr written 3.3 years ago by Genosa • 100 • updated 19 months ago by Kevin Blighe ♦ 56k
0
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0
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1.2k
views
0
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qPCR: Huge variation in fold change of genes between biological replicates
foldchange qpcr written 21 months ago by Ambika • 30 • updated 21 months ago by WouterDeCoster ♦ 43k
1
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0
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683
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5 follow
0
answers
How to statistically compare multiple cell lines
R qpcr written 23 months ago by elb • 170 • updated 23 months ago by Devon Ryan ♦ 94k
1
vote
1
answer
997
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1
answer
Primer design to do qPCR on repetitive elements
repeats repetitive elements qpcr written 2.2 years ago by Gema Sanz • 70 • updated 2.2 years ago by trausch • 1.4k
1
vote
1
answer
971
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1
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Correct for confounding variable (variability in knockdown efficiency) in paired t-test for change in gene expression
t-test gene ancova qpcr statistics written 2.2 years ago by Scott • 80 • updated 2.2 years ago by Devon Ryan ♦ 94k
2
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1
answer
647
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1
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Multiplex gene expression normalization
qpcr written 2.4 years ago by yuggnurug • 70 • updated 2.4 years ago by Kevin Blighe ♦ 56k
2
votes
1
answer
1.9k
views
1
answer
Minimal TPM threshold for qPCR measure of gene expression
tximport tpm qpcr rna-seq kallisto written 2.4 years ago by tlorin • 260
2
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1
answer
1.2k
views
1
answer
primer desing for qPCR on de novo assembly output
rnaseq primer qpcr written 2.6 years ago by pablo61991 • 70 • updated 2.6 years ago by Kevin Blighe ♦ 56k
2
votes
1
answer
959
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1
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Design qpcr primers
primers sequence rna-seq qpcr written 2.6 years ago by sbchua.1990 • 30 • updated 2.6 years ago by James Ashmore • 2.8k
2
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0
answers
799
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0
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Best approach to quantify specific bacteria strain
sequence alignment qpcr snp written 2.7 years ago by CY • 470
0
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0
answers
988
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0
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qPCR to validate gene FPKM is 0 in RNA-Seq
rna-seq qpcr written 2.9 years ago by l0o0 • 200
0
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0
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599
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0
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Different calibrator samples in 2 qPCRs- statistics issue
calibrator qpcr statistics written 3.0 years ago by V24 • 0 • updated 3.0 years ago by Biostar ♦♦ 20
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3
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1.1k
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3
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Statistics for qPCR with varying number of samples between groups
patient samples qpcr statistics written 3.4 years ago by V24 • 0
0
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1
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1.4k
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1
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Quantifying relative ratios of variants by qPCR
qpcr deltadeltact written 3.4 years ago by ThePresident • 140 • updated 3.4 years ago by hazzardwallace • 0
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