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<prev • 153 results • page 1 of 6 • next >
4
votes
1
answer
4.3k
views
1
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How To Set A Threshold On Mapping Quality
quality mapping written 9.2 years ago by Junfeng • 330 • updated 4 weeks ago by caro-ca • 0
4
votes
1
answer
132
views
1
answer
What kind of biological factors can reduce the quality of reads?
quality sequence low sequencing written 3 months ago by ericjanhoekstra • 0 • updated 3 months ago by genomax ♦ 80k
0
votes
0
answers
156
views
0
answers
Read quality only 14
sequencing quality quality score identical fastqc written 3 months ago by ericjanhoekstra • 0 • updated 3 months ago by genomax ♦ 80k
3
votes
1
answer
216
views
1
answer
How to calculate TIN score of the GSE dataset to perform some post quality checks on RNASeq dataset
quality rna-seq written 5 months ago by naseerkhan861 • 0 • updated 5 months ago by i.sudbery ♦ 7.3k
0
votes
0
answers
256
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0
answers
How can I use BBMap reformat.sh to correctly retrieve fastq quality scores from a bam file?
software error reformat.sh quality bam bbmap written 6 months ago by thequokka • 0
2
votes
1
answer
266
views
1
answer
In the reformat tool of BBmap what is the meaning of Read1_linear and Read1_log
reads quality bbmap written 6 months ago by Joseph Hughes • 2.8k • updated 6 months ago by h.mon ♦ 29k
0
votes
2
answers
455
views
2
answers
Trimmomatic is removing excessive reads after trimmimg
trimmomatic quality rna-seq written 7 months ago by sneha108ss • 10
0
votes
0
answers
368
views
0
answers
Fastqc works in terminal fastqc not found during jupyter call
software error assembly quality reads fastqc written 8 months ago by bz3 • 0
3
votes
0
answers
1.9k
views
0
answers
Which Msa Scoring Methods Did You Use?
quality multiple written 8.6 years ago by Aleksandr Levchuk ♦ 3.2k • updated 11 months ago by Biostar ♦♦ 20
2
votes
3
answers
8.3k
views
8 follow
3
answers
Tophat Rna-Seq Mapping Quality
tophat mapping rna-seq quality written 7.0 years ago by siyu • 130 • updated 11 months ago by Biostar ♦♦ 20
51
votes
3
answers
3.6k
views
7 follow
3
answers
What Are Some Sanity Checks That Should Be Performed On Ngs Data?
quality next-gen pipeline sequencing written 9.3 years ago by Jeremy Leipzig ♦ 19k • updated 13 months ago by Biostar ♦♦ 20
4
votes
0
answers
702
views
0
answers
Unexpected hump in GC content distribution from FasQC report in ChIP-seq
quality chip-seq fastqc written 13 months ago by msimmer92 • 250
8
votes
1
answer
1.8k
views
1
answer
Is there a way to do read filtering (MAPQ> certain value) on a BAM instead of SAM file?
quality bam sam filtering mapq written 16 months ago by msimmer92 • 250
0
votes
0
answers
377
views
0
answers
Problem in Sequence Length Distribution
trimmomatic quality sequence lenght fastqc written 15 months ago by vitorgomesbio • 0
12
votes
2
answers
7.2k
views
2
answers
What is a bad GATK Genotype Quality?
quality snp written 4.8 years ago by devenvyas • 620 • updated 16 months ago by Biostar ♦♦ 20
2
votes
1
answer
1.6k
views
1
answer
Assembly With Phrap With Some Missing Quality Files.
fasta assembly quality written 9.8 years ago by Joseph Hughes • 2.8k • updated 19 months ago by RamRS ♦ 26k
13
votes
3
answers
7.1k
views
3
answers
What To Do With An Error In The Fastq Illumina Quality Scores
quality next-gen fastx fastq sequencing written 10.0 years ago by Allpowerde • 1.2k • updated 19 months ago by RamRS ♦ 26k
1
vote
1
answer
537
views
1
answer
miRNA-seq quality drops at certain positions
quality mirna rna-seq fastqc written 19 months ago by kim.malek90 • 0 • updated 19 months ago by genomax ♦ 80k
2
votes
1
answer
541
views
1
answer
Metagenomics: rationale behind Q trimming
illumina metagenomics bbduk quality trimming written 20 months ago by f.a.galkin • 40 • updated 20 months ago by finswimmer ♦ 13k
81
votes
9
answers
25k
views
31 follow
9
answers
How To Assess The Quality Of An Assembly? (Is There No Magic Formula?)
assembly quality next-gen written 7.2 years ago by diltsjeri • 440 • updated 21 months ago by alslonik • 130
3
votes
2
answers
1.1k
views
2
answers
Difference between marking duplicates and filtering BAM on phred score
duplicates next-gen picard quality alignment written 22 months ago by Nalini • 10 • updated 22 months ago by d-cameron • 2.1k
4
votes
1
answer
510
views
1
answer
Microarray Probesets Quality Control - Why exclude non-autosomal probesets?
control quality autosomes microarray probesets written 22 months ago by Volka • 70 • updated 22 months ago by Devon Ryan ♦ 94k
2
votes
1
answer
891
views
1
answer
Per tail sequence quality fail
quality tile fastqc written 22 months ago by misterie • 90 • updated 22 months ago by Devon Ryan ♦ 94k
1
vote
1
answer
490
views
1
answer
Removal of non quality read in fastq file
fastq quality written 22 months ago by akbioinfo14 • 0 • updated 22 months ago by Bastien Hervé ♦ 4.5k
0
votes
1
answer
639
views
1
answer
Decreased base quality at start of RNA seq reads
base quality position rna-seq sequencing written 22 months ago by brismiller • 20 • updated 22 months ago by Wietje • 210
0
votes
0
answers
848
views
0
answers
Per base sequence quality and per base N content
quality fastqc written 22 months ago by misterie • 90
2
votes
1
answer
1.4k
views
1
answer
Is there any alternative to QUAST software to evaluate de novo assembly results?
assembly quality evaluation quast written 2.1 years ago by oxfordsoccer • 10 • updated 2.1 years ago by Sej Modha ♦ 4.6k
1
vote
1
answer
643
views
1
answer
Opinion on Quality basecall threshold to use
vcf filter quality qual written 2.3 years ago by ricfoz • 30
0
votes
0
answers
1.1k
views
0
answers
How to improve alignment quality of ChIP-seq for single-end
bowtie2 quality alignment chip-seq single-end written 2.3 years ago by jule • 0
0
votes
0
answers
888
views
0
answers
Quality controls with plink
controls quality plink written 2.3 years ago by vgenepi • 10
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