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15
votes
4
answers
228
views
7 follow
4
answers
Is it possible to analyze differentially expressed genes without sample replicates using DESeq2?
deseq2 rna-seq written 4 weeks ago by Sarath Kumar • 0 • updated just now by bloggerklik • 0
0
votes
1
answer
17
views
1
answer
GFF file multiple features for 1 gene region, how to collapse into 1?
bedtools gff rna-seq written 1 hour ago by YOUSEUFS • 10 • updated 44 minutes ago by Carambakaracho • 550
3
votes
0
answers
82
views
6 follow
0
answers
allele specific expression (ASE) reference ratio plot query
allele-specific expression rna-seq snp written 11 hours ago by prasundutta87 • 250
0
votes
0
answers
35
views
0
answers
Genome and transcriptome analyses in yeast
snp rna-seq sequencing written 4 hours ago by JJ • 360
0
votes
0
answers
42
views
0
answers
RNA-Seq normalization using limma or DESeq2
normalization limma rna-seq dseq2 written 3 hours ago by user56 • 0
4
votes
1
answer
111
views
1
answer
Problem with extracting read counts from RNA seq file
software error featurecounts rna-seq rseqc written 1 day ago by popescuiofelia • 0 • updated 5 hours ago by h.mon ♦ 20k
2
votes
2
answers
346
views
2
answers
HISAT2 HLA genotyping errors
genome software error rna-seq written 7 months ago by jgibbs • 20 • updated 6 hours ago by okaMavuso • 0
1
vote
0
answers
67
views
0
answers
Job: Bioinformatics Data Scientist and Postdoctoral Fellow - Xing lab @ CHOP
splicing job rna-seq written 8 hours ago by Jeremy Leipzig ♦ 17k
0
votes
0
answers
53
views
0
answers
Single molecule RNA-seq, without poly(A) screening.
rna-seq written 11 hours ago by gauravvman • 0
0
votes
0
answers
37
views
0
answers
monocle how to identify branches
monocle tool pseudotime rna-seq written 17 hours ago by hsu • 0
14
votes
2
answers
6.8k
views
11 follow
2
answers
samtools: error while loading shared libraries: libbz2.so.1.0: cannot open shared object file: No such file or directory
samtools rna-seq written 14 months ago by wschrein • 20 • updated 18 hours ago by 1176423710 • 0
1
vote
0
answers
90
views
5 follow
0
answers
To fetch the reads which are being partially mapped to the genome
jvarkit hisat2 star rna-seq bowtie2 written 1 day ago by swadha • 20 • updated 23 hours ago by genomax ♦ 57k
0
votes
1
answer
73
views
1
answer
GC content analysis using EdgeR
rna-seq written 1 day ago by vhelizarraga • 0 • updated 1 day ago by h.mon ♦ 20k
2
votes
2
answers
131
views
2
answers
Time-course RNA-seq analysis in Deseq2
R rna-seq written 1 day ago by Lisa • 310 • updated 1 day ago by Vitis • 1.6k
0
votes
1
answer
90
views
1
answer
Infer direction of paired end data
rna-seq written 1 day ago by waqaskhokhar999 • 0 • updated 1 day ago by Istvan Albert ♦♦ 77k
2
votes
0
answers
118
views
5 follow
0
answers
gene expression for cancer identification
gene rna-seq written 1 day ago by maryam.akram001 • 10
0
votes
0
answers
61
views
0
answers
reliability for the usage of merged GTF from tophat-cufflinks as an novel annotation?
cufflinks mats rna-seq alternative splicing written 1 day ago by thindmarsmission • 0
0
votes
1
answer
113
views
1
answer
Is TCGA Breast cancer data strand specific?
geneexpression tcga rna-seq strand hisat2 written 1 day ago by Bioinfo • 270 • updated 1 day ago by genomax ♦ 57k
2
votes
0
answers
83
views
0
answers
What is the ideal FDR and fold change cutoff to consider in a RNA-seq experiment and which one should be the priority for selecting the DEGs?
fdr deseq2 rna-seq deg fold change written 1 day ago by aishu.jp • 10
0
votes
0
answers
84
views
0
answers
News: Fully funded PhD studentships in Quantitative Biology, Universite de Lausanne, Lausanne, Switzerland
genome R news rna-seq snp written 1 day ago by cara • 0 • updated 1 day ago by genomax ♦ 57k
2
votes
1
answer
2.3k
views
1
answer
TCGA/GDC FPKM vs FPKM-UQ
gdc rna-seq written 19 months ago by igor ♦ 6.8k • updated 1 day ago by gabriel.rosser • 130
0
votes
0
answers
276
views
0
answers
Should I remove samples after normalization of miRNA seq read counts ?
rna-seq edger mirnas written 8 months ago by Björn • 30 • updated 1 day ago by Biostar ♦♦ 20
2
votes
0
answers
153
views
0
answers
RNASeq - low correlation between samples
R correlation rna-seq written 3 days ago by pentium3-user • 20 • updated 1 day ago by zx8754 ♦ 5.3k
5
votes
3
answers
1.8k
views
3
answers
High Kmer Content in middle of the read
rna-seq written 2.9 years ago by Sam • 2.2k • updated 1 day ago by jgarces • 0
1
vote
0
answers
52
views
0
answers
How to remove batch effects when i have different organs?
rna-seq written 1 day ago by jyshiningsoul • 0
0
votes
0
answers
163
views
0
answers
Gene regulatory networks from expression data
network rna-seq written 5 weeks ago by pixie@bioinfo • 1.3k • updated 1 day ago by Biostar ♦♦ 20
2
votes
1
answer
89
views
1
answer
Gene expression distribution (non-DE and DE)
rna-seq differential expression written 2 days ago by CY • 200 • updated 1 day ago by Kevin Blighe ♦ 30k
0
votes
1
answer
87
views
1
answer
The difference between heatmap.2 and pheatmap
rna-seq heatmap written 2 days ago by wangdp123 • 120 • updated 1 day ago by Kevin Blighe ♦ 30k
3
votes
1
answer
98
views
1
answer
Unsupervised selection of inter-cluster highly variable genes
variance R job rna-seq written 2 days ago by elb • 140 • updated 1 day ago by Kevin Blighe ♦ 30k
0
votes
1
answer
112
views
1
answer
Plot of 2 replicates
R rna-seq written 2 days ago by dianalves17 • 0 • updated 2 days ago by Russ • 410
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