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Recent Replies
Answer: Create a new seurat object using one cluster identified in a previous seurat obj
by
Ribo
▴
20
To subset your data based on cell identity, use:
new_seurat_object <- original_seurat_object %>% subset(idents = 'cluster_name')
Comment: How to add a individuals IDs to admixture output?
by
Brittany
•
0
Hi there! I was wondering if any of you knew how to tell which of the populations were represented in the Q file. Like in your data set, do…
Comment: RNAseq for DE purpose
by
Ram
37k
You need your command to run once per pair of files, not combine all pairs and then run just once. Read the thread you're trying to emulate…
Comment: RNAseq for DE purpose
by
m.habib
•
0
I basically changed the file names to make sure this command is working. I picked one forward and reverse read pair and processed it as bel…
Comment: RNAseq for DE purpose
by
m.habib
•
0
Here is an example:
19144R-03-01_S75_L004_R2_001.fastq.gz
Comment: RNAseq for DE purpose
by
GenoMax
125k
What names do your fastq files have? That first part of my answer will need to be adjusted to account for the differences in your file name…
Comment: Analyzing bulk RNA-seq
by
seidel
10k
A bulk RNA Seq data set is not a single cell RNA data set, and there is no R package that can convert bulk data to single cell data. If you…
Comment: Why am I getting only one common value when comparing two excel files?
by
seidel
10k
According to your code, you asked for a set of common values, and that's what it returned. Are you trying to merge the two data frames with…
Answer: Why am I getting only one common value when comparing two excel files?
by
seidel
10k
You mentioned fixing the case of the values shared by each table, so that they will match. If you'd like to join the two tables together, y…
Comment: RNAseq for DE purpose
by
m.habib
•
0
I used the following:
java -jar trimmomatic-0.39.jar PE -phred33 -threads 4 $i\_R1_001.fastq.gz $i\_R2_001.fastq.gz $i\_R1_001¬_paire…
Comment: RNAseq for DE purpose
by
Ram
37k
What thread did you copy it from, what changes did you make and what was the logic behind each of those changes?
Answer: Alien-hunter outputs
by
schlogl
▴
120
I think I got a small explanation in a MSC dissertation here in Brazil. but if you guys have any other source I appreciate. Thanks
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