Recent Votes
Recent Locations •
All
United States, just now
São Paulo, 1 minute ago
United Kingdom, 1 minute ago
Germany, 1 minute ago
United States, 2 minutes ago
Norway, 2 minutes ago
United States, 2 minutes ago
Recent Awards •
All
Popular Question to
chi.delta
▴
40
Appreciated to
Sasha
▴
260
Popular Question to
lait
▴
170
Popular Question to
blur
▴
270
Popular Question to
Tawny
▴
180
Scholar to
rpolicastro
12k
Recent Replies
Answer: counts matrix should be numeric, currently it has mode: character when using a c
by
ATpoint
72k
You have commas as decimal delimiters which interpreted as text. Just replace by dot and make numeric:
```r
d <- data.frame(A=c("1,2", "3,…
Comment: sgRNA design
by
GenoMax
130k
Library fragments can be in forward/reverse orientation (w.r.t reference sequence) so it is possible to see sequence from either strand in …
Comment: snps from empty well, Illumina Sequencing
by
GenoMax
130k
> I receive illumina sequencing files with 96 wells, our lab leaves one
> well empty. When I process the files with a SNP calling pipeline …
Answer: counts matrix should be numeric, currently it has mode: character when using a c
by
Darked89
4.4k
You can try:
counts_data <- read.csv2("normalized.csv", header = TRUE)
But as far as I know DESeq2 requires raw counts as inpu…
Comment: Is there bioinformatics tool to check cross hybridization between primers and pr
by
Tawny
▴
180
Was a tool ever found for this? I am curious.
Comment: Is the Kruskal-Wallis test appropriate for genomic distances?
by
ATpoint
72k
I never used it myself so far, but I would proably look into https://bioconductor.org/packages/devel/bioc/vignettes/nullranges/inst/doc/nul…
Comment: ChatGPT optimized for bioinformatics questions
by
GenoMax
130k
Since you have kept the bot openly accessible (at least for now) this is great. People can try it out and hopefully provide feedback. I tri…
Comment: How to use R installed by conda instead of it installed by apt on Ubuntu
by
jv
★
1.2k
if you are doing these steps then the `R` version should be `4.1.1`. Are you sure there weren't any errors during the install of `r-base=4.…
Comment: How to use R installed by conda instead of it installed by apt on Ubuntu
by
Ram
39k
Please follow this sequence of steps and show us the outputs:
```
# Start from a fresh terminal with no conda environment active:
which -a…
Comment: phage genome submission in ncbi genebank
by
tahsinkhan570
•
0
Thanks for the info. I called the --compliant and later removed the Ec_number of hypothetical protein as mentioned by NCBI table2asn guidel…
Comment: ChatGPT optimized for bioinformatics questions
by
Sasha
▴
260
Great use case - we'll take a look. Thanks for trying it out!
Comment: retaining only the clusters of interest
by
Ram
39k
You're close. The `subset` function serves a similar functionality as the base, but since the Seurat object is an S3 object, it subsets bas…
Answer: ChatGPT optimized for bioinformatics questions
by
Ram
39k
I asked the same questions to tinyBio and chatGPT and chatGPT gave me better answers. This could be because chatGPT has been out for a whil…
Comment: ChatGPT optimized for bioinformatics questions
by
Sasha
▴
260
Hi GenoMax - it's primed on Github repos, some personality tuning, and some prompt assumptions based on the context of the site being bioin…
Traffic: 3263 users visited in the last hour
Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by the
version 2.3.6
version 2.3.6