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Recent Votes
- A: Analyse RNA-seq differential expression data
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Recent Replies
-
C: No differential gene expression after tuxedo protocol by
Radek • 830
If you see the mapping of the reads (with IGV for example) and bedtools is not working you could ...
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C: No differential gene expression after tuxedo protocol by
vinayjrao • 60
Dear Radek, I have 2 cell line and 2 animal models, both with a cancer and a normal data set. I w...
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C: Denisovan and Altai Neanderthal .bed files? by
spiral01 • 60
Thank you. As the Neanderthal and Denisovan sequences are mapped to hg19, does this mean I can us...
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C: No differential gene expression after tuxedo protocol by
vinayjrao • 60
I'm currently trying hisat2 protocol, but haven't finished it yet, so I couldn't use it. And rega...
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C: UTR size range for 5' UTR & 3' UTR variants by
anupriyaverma1408 • 100
Thanks Emily. That cleared my understanding about UTRs & scripting :)
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C: Rename headers of multiple fasta files inside loop by
bioinfo8 • 80
@[st.ph.n][1] I explained my query in more detail in above comment. I hope its more clear now. ...
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A: Appropriate peak length of ChIP-seq. (Histone modification having different patt by
geek_y ♦ 7.8k
Applying `--broad` does not effect calling sharp peaks in MACS2. As you said, its essentially cal...
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A: No differential gene expression after tuxedo protocol by
Buffo • 470
May be you want to read this: [Transcript-level expression analysis of RNA-seq experiments with H...
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C: Is it possible to annotate single genes by snpeff by
WouterDeCoster ♦ 20k
Like previous threads: EXPLAIN MORE. Give information about what you are doing (MSA of multiple s...
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A: Gene extraction from documents by
Emily_Ensembl ♦ 12k
This is what Europe PMC use: https://europepmc.org/Annotations
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C: No differential gene expression after tuxedo protocol by
Radek • 830
More up to date differential expression pipeline exist (DESeq2, limma-voom, kalisto+sleut, etc). ...
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C: No differential gene expression after tuxedo protocol by
Satyajeet • 790
I agree with @Devon. However, your samples seem to suffer from another issue here. can you check ...
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