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How to process degenerate base region (DBRs) in a ddRAD sequencing protocol (remove PCR duplicates)?
written 5 days ago by
beausoleilmo • 140
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written 14 months ago by
arash.askary • 10
• updated
14 months ago by
natasha.sernova • 2.5k
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Recent Replies
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C: Where can I find DNase-seq assays of cell line MCF-10A? by
Kevin Blighe • 1.3k
Have you tried searching the Sequence Read Aechive? - https://www.ncbi.nlm.nih.gov/sra
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C: Extracting chimeric reads from mapping by
Anushka • 20
yes that make sense. Thank you so much for your help
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Looks like I found the solution :) x <- IlluminaHumanMethylation27kSYMBOL # Get the...
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Looks great! Yes, labeling is a major issue, but there are different ways of tackling it: #Modi...
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Charles Plessy • 2.2k
Just for the record: FastUniq can not account for sequencing errors (which can be a strong limita...
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C: how to resolve this 1-bases cordinates confusion by
genomax ♦ 33k
What genome build is this from? [According to Ensembl FABP5][1] is a pseudo-gene in Dog (CanFam ...
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A: how to resolve this 1-bases cordinates confusion by
Alex Reynolds ♦ 20k
> BED and my BAM file is 1-based BED and BAM are usually 0-based, half-open `[start-1, end)`....
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A: how to resolve this 1-bases cordinates confusion by
jrj.healey • 2.5k
If I understand the problem then I'm not sure you can easily resolve this without more informatio...
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C: exon level quantification using htseq count by
komal.rathi • 2.9k
Oh I just realized I don't have the `exon_id` columns in the gff while I am passing `exon_id` to ...
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C: how to resolve this 1-bases cordinates confusion by
adeenahassan77 • 30
ohhhh sorry 4 that :o above example not from published genome. but i have also tried it for dog...
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C: how to resolve this 1-bases cordinates confusion by
jrj.healey • 2.5k
But you said you downloaded it from NCBI?
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Thanks! Will get to it. Right now still struggling to find a way to analyze the data. Though it's...
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