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379 results • Page
3 of 8
Sort: Votes
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Views
Votes
Replies
19
votes
2
replies
3.2k
views
Tutorial:
Beginner's Handbook to Next Generation Sequencing
NGS
updated 4 months ago by
Ram
40k • written 6.5 years ago by
support
▴ 640
19
votes
3
replies
13k
views
Tutorial:
bedGraphToBigWig Tutorial and Report
bedGraphToBigWig
updated 14 months ago by
Ram
40k • written 7.6 years ago by
Shicheng Guo
★ 9.3k
19
votes
4
replies
8.6k
views
Tutorial:
Rna Seq Experimental Design For Measuring Differential Gene Expression
RNA-seq
updated 7 months ago by
Ram
40k • written 10.6 years ago by
Istvan Albert
98k
19
votes
13
replies
6.8k
views
9 follow
Tutorial:
Setting up Aspera Connect (ascp) on Linux and macOS
ascp
aspera
updated 23 hours ago by
dariober
14k • written 15 months ago by
ATpoint
77k
18
votes
2
replies
9.7k
views
Tutorial:
Video: RNA-Seq Alignment and Visualization using Galaxy and IGB
RNA-Seq
Galaxy
IGB
updated 18 months ago by
Ram
40k • written 8.9 years ago by
Nowlan Freese
▴ 860
18
votes
7
replies
5.7k
views
Tutorial:
scRNA-seq CITE-seq-count bioinformatics
CITE-seq-count
scRNA-seq
RNA-Seq
updated 10 weeks ago by
ATpoint
77k • written 3.5 years ago by
colindaven
5.5k
18
votes
40
replies
17k
views
16 follow
Tutorial:
Easy way to run easily orthoMCL (Copy & paste)
all-v-all
orthomcl
updated 6 months ago by
Ram
40k • written 7.3 years ago by
Esaie
▴ 170
18
votes
2
replies
5.7k
views
Tutorial:
Update kraken databases
database
kraken
k-mer
python
updated 4 months ago by
Ram
40k • written 6.4 years ago by
Sej Modha
5.2k
18
votes
22
replies
8.1k
views
10 follow
Tutorial:
Create de novo repeat library
de-novo
repeat
annotation
updated 7 days ago by
evoecogen
▴ 20 • written 3.8 years ago by
Juke34
8.2k
17
votes
13
replies
1.9k
views
Tutorial:
Screen shots of text (please don't)!
meta
updated 4 months ago by
Ram
40k • written 6.5 years ago by
Michael
53k
16
votes
11
replies
7.7k
views
Tutorial:
How to Use Biostars, Part II: Post types, Deleting, (Un)Subscribing, Linking and Bookmarking
meta
how-to
documentation
6 months ago by
Ram
40k
16
votes
7
replies
14k
views
Tutorial:
Integrating VDJ sequencing data with Seurat
VDJ
Seurat
sc-RNAseq
clonotyping
updated 6 months ago by
Ram
40k • written 4.3 years ago by
atakanekiz
▴ 310
16
votes
5
replies
8.7k
views
Tutorial:
Using The Biomart Perl Api For Simple Queries
api
perl
biomart
updated 7 months ago by
Ram
40k • written 11.0 years ago by
Obi Griffith
20k
16
votes
0
replies
12k
views
Tutorial:
Workshop - Analysis Of Next Generation Sequencing Data
RNA-seq
updated 7 months ago by
Ram
40k • written 11.5 years ago by
David Langenberger
10k
16
votes
9
replies
5.8k
views
Tutorial:
TCGA transcriptome data to R (DESeq2)
TCGA
GDC
DESeq2
updated 15 months ago by
dk0319
▴ 70 • written 22 months ago by
Barry Digby
★ 1.2k
16
votes
6
replies
6.1k
views
Tutorial:
How to make your snakefiles self-documenting
snakemake
updated 5 months ago by
Ram
40k • written 6.9 years ago by
Botond Sipos
★ 1.7k
16
votes
0
replies
6.5k
views
Tutorial:
Introduction To Ngs Bioinformatics By The Bioinformatics Team At The University Of Texas
course
training
updated 7 months ago by
Ram
40k • written 11.3 years ago by
Istvan Albert
98k
16
votes
11
replies
14k
views
7 follow
Tutorial:
retrieve full TCGA datasets from cBioportal with R
TCGA
RNA-seq
data-retrieval
cBioportal
R
updated 6 months ago by
Ram
40k • written 6.9 years ago by
TriS
★ 4.7k
15
votes
1
reply
6.7k
views
Tutorial:
A compilation of conversion tools for BED, SAM/BAM, psl, pslx, blast tabular and blast xml
psl
SAM
blast
BED
updated 4 months ago by
Ram
40k • written 5.9 years ago by
Joseph Hughes
★ 3.0k
15
votes
5
replies
15k
views
Tutorial:
How to create a mutation lolliplot with GenVisR
bioconductor
graphics
R
genvisr
updated 5 months ago by
Ram
40k • written 7.3 years ago by
Obi Griffith
20k
15
votes
1
reply
3.2k
views
Tutorial:
Dealing with nanopore fast5 files compressed with vbz
hdf5
nanopore
fast5
vbz
MinKNOW
updated 13 months ago by
Assa Yeroslaviz
★ 1.8k • written 21 months ago by
dariober
14k
15
votes
2
replies
6.7k
views
Tutorial:
Genotype And Snp Calling Review Papers
snp
updated 7 months ago by
Ram
40k • written 11.1 years ago by
Istvan Albert
98k
15
votes
4
replies
4.3k
views
Tutorial:
RNASeq tutorial for gene differential expression analysis
Transcriptomics
bioconductor
RNA-Seq
R
updated 6 months ago by
Ram
40k • written 3.2 years ago by
Thind amarinder
▴ 330
15
votes
5
replies
12k
views
6 follow
Tutorial:
Easy way to do GO enrichment in R
enrichr
RNA-Seq
gene
R
updated 6 months ago by
Ram
40k • written 5.8 years ago by
ahmad mousavi
▴ 760
15
votes
1
reply
3.0k
views
Tutorial:
Current Topics in Genome Analysis 2016 (collection of educational lectures)
genomics
training
lectures
workshop
updated 5 months ago by
Ram
40k • written 7.4 years ago by
Malachi Griffith
19k
15
votes
2
replies
3.4k
views
Tutorial:
Methylation Analysis Tutorial in R_part2
cancer
methylation
RNA-Seq
R
updated 6 months ago by
Ram
40k • written 3.0 years ago by
Hamid Ghaedi
3.0k
15
votes
2
replies
1.8k
views
Tutorial:
Germline variant calling pipeline using Snakemake
snakemake
GATK
germline_variant_calling
updated 11 months ago by
brunobsouzaa
▴ 810 • written 2.0 years ago by
nhaus
▴ 280
15
votes
3
replies
4.4k
views
Tutorial:
NCBI blast tutorial
ncbi
blast
updated 5 months ago by
Ram
40k • written 7.4 years ago by
Eric Normandeau
11k
15
votes
2
replies
2.8k
views
Tutorial:
Introduction to bedtools.
bedtools
updated 6 months ago by
Ram
40k • written 8.9 years ago by
Aaronquinlan
12k
14
votes
2
replies
2.3k
views
Tutorial:
Exploring OpenSNP - an RMarkdown report on crowdsourced genotypes and phenotypes
SNP
R
updated 5 months ago by
Ram
40k • written 7.5 years ago by
Jeremy Leipzig
21k
14
votes
0
replies
8.1k
views
Tutorial:
Video: ChIP-Seq Analysis and Visualization using Galaxy and IGB.
IGB
ChIP-Seq
Galaxy
MACS
RNA-Seq
updated 18 months ago by
Ram
40k • written 8.7 years ago by
Nowlan Freese
▴ 860
14
votes
2
replies
6.3k
views
Tutorial:
ChIP-seq analysis tutorial
ChIP-Seq
updated 6 months ago by
Ram
40k • written 8.1 years ago by
Ming Tommy Tang
★ 3.4k
14
votes
0
replies
9.2k
views
Tutorial:
How to generate PCA plot and heatmaps in R
heatmap
PCA
RNA-Seq
R
updated 5 months ago by
Ram
40k • written 7.0 years ago by
Ming Tommy Tang
★ 3.4k
14
votes
7
replies
5.9k
views
Tutorial:
Visualization of ChIP-Seq peak overlaps using HOMER mergePeaks and UpSetR
ChIP-Seq
peaks
R
HOMER
updated 6 months ago by
Ram
40k • written 7.4 years ago by
steve
★ 3.3k
13
votes
3
replies
11k
views
Tutorial:
Plotting the coverage and depth (Y-axis) statistics of a bam file along the genome (X-axis), using Samtools, awk and R.
genome
plot
coverage
samtools
updated 6 weeks ago by
William
▴ 20 • written 6.5 years ago by
kirannbishwa01
★ 1.6k
13
votes
1
reply
2.6k
views
Tutorial:
PCR Primer or Primer+Probe design
pcr
probe
rt-pcr
qpcr
primer
updated 2.3 years ago by
jared.andrews07
★ 15k • written 2.3 years ago by
Kevin Blighe
86k
13
votes
6
replies
5.6k
views
Tutorial:
Trim & align paired-end reads in a single pass
align
trim
fastq
pipe
paired-end
updated 5 months ago by
Ram
40k • written 7.3 years ago by
dariober
14k
13
votes
3
replies
4.9k
views
Tutorial:
Video: Visualization of whole genome DNA methylation data in IGB
Bismark
BS-Seq
RNA-Seq
IGB
updated 16 months ago by
Ram
40k • written 8.5 years ago by
Nowlan Freese
▴ 860
13
votes
31
replies
5.7k
views
6 follow
Tutorial:
gene set filter/selection for training ab initio annotation tools
geneset
genome
gene
abinitio
updated 6 months ago by
Ram
40k • written 4.1 years ago by
Juke34
8.2k
12
votes
1
reply
4.9k
views
Tutorial:
RepSeq data processing and analysis
immunology
tcr
repseq
sequencing
repertoire
updated 11 months ago by
Ram
40k • written 8.1 years ago by
mikhail.shugay
3.5k
12
votes
17
replies
2.5k
views
Tutorial:
How to find the mapping percentages for data deposited in the Zaire ebolavirus bioproject from the 2014 outbreak
ebola
bwa
updated 9 months ago by
Ram
40k • written 8.3 years ago by
Istvan Albert
98k
12
votes
20
replies
13k
views
10 follow
Tutorial:
Associating VDJ clonotyping data with scRNA-seq in Seurat
single-cell
RNA-Seq
seurat
TCR
updated 6 months ago by
Ram
40k • written 4.3 years ago by
jared.andrews07
★ 15k
12
votes
4
replies
2.0k
views
Tutorial:
Tutorials for Library Normalization in DESeq2 and EdgeR
EdgeR
DESeq2
updated 6 months ago by
Ram
40k • written 6.1 years ago by
KVC_bioinfo
▴ 590
12
votes
5
replies
2.1k
views
Tutorial:
FASTA file split
fasta
updated 6 months ago by
Ram
40k • written 3.3 years ago by
Juke34
8.2k
12
votes
4
replies
5.0k
views
Tutorial:
*FREE course* Bioinformatics for Biologists: An Introduction to Linux, Bash Scripting, and R
linux
R
updated 7 months ago by
Ram
40k • written 2.6 years ago by
garden_giessen
▴ 130
12
votes
7
replies
5.3k
views
Tutorial:
STARsolo config for 10x Chromium v1, v2, v3
Chromium
10x
STARsolo
STAR
updated 6 months ago by
Ram
40k • written 3.0 years ago by
Kevin Blighe
86k
12
votes
5
replies
7.9k
views
Tutorial:
How To Install BioPerl Without Root Privileges in Ubuntu/Linxu
bioperl
updated 5 months ago by
Ram
40k • written 7.3 years ago by
Shicheng Guo
★ 9.3k
12
votes
11
replies
7.0k
views
Tutorial:
How to upload your RNA-Seq data to NCBI Sequence Read Archive (SRA)
NCBI
Bioproject
SRA
BioSample
updated 4 months ago by
Ram
40k • written 6.6 years ago by
CandiceChuDVM
★ 2.4k
12
votes
0
replies
7.5k
views
Tutorial:
Plot average methylation levels across TSS region
bedtools
RRBS
R
methylation
updated 17 months ago by
Ram
40k • written 8.7 years ago by
TriS
★ 4.7k
12
votes
0
replies
2.4k
views
Tutorial:
Long versus wide data for plotting genomics data in R.
ggplot2
tidyverse
data.table
R
updated 6 months ago by
Ram
40k • written 3.1 years ago by
rpolicastro
12k
379 results • Page
3 of 8
Recent Votes
A: running RAxML and MrBayes over concatinated multiple sequence alignments
Comment: Weirdness in annotation (missing allele frequencies)
Comment: Weirdness in annotation (missing allele frequencies)
Comment: ncbi error report log for validate fastq issue
Comment: ncbi error report log for validate fastq issue
A: Bowtie2 MAPQ difference using local vs end-to-end alignment
C: Bowtie2 MAPQ difference using local vs end-to-end alignment
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Comment: Weirdness in annotation (missing allele frequencies)
by
Jeremy Leipzig
21k
0.60625 is 97/160 so if you have 80 individuals in your VCF that's an internal allele frequency, not gnomAD
Comment: Weirdness in annotation (missing allele frequencies)
by
Can Abdullah
• 0
Yes, I was confused as well. I think the same. Maybe Annovar uses some other resources besides gnomad, but it does not make sense. Also, I …
Comment: Weirdness in annotation (missing allele frequencies)
by
Can Abdullah
• 0
Thank you so much, I decided to move forward as you suggested. Initially, I thought using the most up-to-date databases and positions would…
Comment: Getting the overlap between two GTF files
by
GenoMax
134k
Something in `AGAT` should work: https://agat.readthedocs.io/en/latest/?badge=latest
Answer: Automate the Splitting of a VCF File by Sample (bcftools)
by
Pierre Lindenbaum
157k
> My problem has been with trying to loop the process. ok here is a **nextflow** based solution,(NOT tested) workflow { …
Comment: Assessing Rockhopper's output
by
langziv
▴ 50
I see. I tried using the GUI Rockhopper for RNA-seq analysis of a K. pneumoniae strain, but the program didn't find the strain's assembly…
Answer: Getting the overlap between two GTF files
by
Pierre Lindenbaum
157k
bedtools intersect \ -a <(awk '/^[^#]/ {printf("%s\t%d\t%s\t%s\n",$1,int($4)-1,$5,$0);}' file1.gtf | sort -t $'\t' -k1,1 -k2…
Comment: Assessing Rockhopper's output
by
shelkmike
▴ 980
I used Rockhopper only for bacterial genomes assembled into circular contigs or circular scaffolds.
Comment: Unable to install HorvathMammalMethylChip40manifest packages
by
ATpoint
77k
There is no package of that name in CRAN or Bioconductor. Where do you have it from, so which tutorial you follow?
Comment: Subclustering of intergated cells from scRNA-seq data
by
fifty_fifty
▴ 60
thank you. This is exactly what I was looking for
Answer: Search RCSB with a list of protein names?
by
Mensur Dlakic
★ 25k
This is not the most elegant solution, but it should work with previous suggestions. In this remote directory: http://ftp.wwpdb.org/p…
Answer: Automate the Splitting of a VCF File by Sample (bcftools)
by
Ram
40k
The manual says you can split samples out at once using the `+split` plugin. So, `bcftools +split -Oz -o <PATH> file.vcf.gz` should do the …
Comment: differences between trajectories in conditions with Monocle3 or other tools
by
Amitm
★ 2.2k
Phate (or Monocle) doesn't do UMAP. If you have done UMAP already (using seurat) then you could use the cell barcode to UMAP cluster label …
Comment: Error: cannot open file 2 for reading From Cufflinks Version 2.2.1 When Attempti
by
Y
• 0
I figured out how to make it work. The bams have to be comma separated not just by space: cd "${input_directory_with_associated_bam_fi…
Comment: Search RCSB with a list of protein names?
by
Joseph
• 0
Hey, this is so close to what I'm looking for - I clarified in my edit. This is a great way to batch download lots of PDBs in RCSB by their…
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